| 期刊全稱 | Artificially Controllable Nanodevices Constructed by DNA Origami Technology | | 期刊簡稱 | Photofunctionalizati | | 影響因子2023 | Yangyang Yang | | 視頻video | http://file.papertrans.cn/163/162734/162734.mp4 | | 發(fā)行地址 | Nominated by Kyoto University as an outstanding Ph.D. thesis.Introduces detailed protocols of larger-sized DNA origami assembling and especially photo functionalization.Presents new methods for the ob | | 學科分類 | Springer Theses | | 圖書封面 |  | | 影響因子 | .In this book, the author deals mainly with two topics: (1) single-molecule visualization of switching behaviors in the DNA nanoframe system utilizing different kinds of molecular switches through the use of high-speed atomic force microscope (AFM); (2) construction of photocontrollable DNA nanostructures in programmed patterns and direct visualization of the dynamic assembling process. Here, high-speed AFM was employed to observe the dynamic movements of single molecules. Compared to a traditional single-molecule analysis method, such as fluorescence spectroscopy or electron microscopy, high-speed AFM makes possible the real-time observation of molecule behaviors. DNA nanostructures were designed and assembled as scaffolds to incorporate interested biomolecules. The observations were carried out under robust conditions without complicated pretreatment. Moreover, the photoresponsive molecules were successfully assembled into around 100 nm-sized DNA nanostructures. The assembly/disassembly of nanostructures can be regulated reversibly by photoirradiation. This book explains how DNA origami has gradually become a useful tool for the investigation of biochemical interactions in define | | Pindex | Book 2015 |
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