Titlebook: Artificial Riboswitches; Methods and Protocol Atsushi Ogawa Book 2014 Springer Science+Business Media New York 2014 RNA aptamers.artificial

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Extrahuman Transcendence in Oscar Wilde’s ools have a simple stem–loop structure. We present here a computational approach for designing a starting library of RNA sequences with increased formation of complex structural motifs and enhanced affinity to a desired target molecule. Our approach consists of two steps: (1) generation of RNA seque

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Plato’s , as an Ethical Performancedesigned and engineered using existing RNA aptamers that target proteins. Aptamers are generated via an iterative selection–amplification process, known as systematic evolution of ligands by exponential enrichment (SELEX). This chapter describes a SELEX procedure for the identification of RNA aptame

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Fictional Worlds and the Moral Imagination their utility, methods are required to create custom riboswitches de novo or to modify existing riboswitches to suit specific experimental needs. This chapter describes such a method, which exploits fluorescence-activated cell sorting (FACS) to quickly and efficiently sort through large libraries o

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Fictional Worlds and the Moral Imaginationseful tools to reprogram cellular behavior for different applications. A new synthetic riboswitch can be created by linking an in vitro-selected aptamer with a randomized expression platform followed by in vivo selection and screening. Here, we describe an in vivo selection and screening technique t

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