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Titlebook: Arthritis Research; Volume 1: Methods an Andrew P. Cope Book 2007 Humana Press 2007 Arthritis.PCR.gene expression.gene transfer.genes.hybri

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發(fā)表于 2025-3-21 17:16:28 | 只看該作者 |倒序瀏覽 |閱讀模式
期刊全稱Arthritis Research
期刊簡稱Volume 1: Methods an
影響因子2023Andrew P. Cope
視頻videohttp://file.papertrans.cn/162/161918/161918.mp4
發(fā)行地址Broad range of topics covering many aspects of rheumatic disease.In-depth studies of cutting-edge technology.Robust tools for translational research.Contributions from leaders in their fields
學科分類Methods in Molecular Medicine
圖書封面Titlebook: Arthritis Research; Volume 1: Methods an Andrew P. Cope Book 2007 Humana Press 2007 Arthritis.PCR.gene expression.gene transfer.genes.hybri
影響因子.Arthritis Research: Methods and Protocols is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. These volumes details novel technologies, some of which are still evolving and whose impacts are yet to be determined. Leaders in the field contribute to cover such exciting and cutting edge topics as imaging and immunohistochemistry, analysis of cartilage and bone catabolism, immunobiology, and cell trafficking. ..In Volume 1, authors discuss synovial joint morphology, histopathology, and immunohistochemistry, cartilage matrix and bone biology, and cell trafficking, migration and invasion. Volume 2 is broken up into sections that cover immunobiology and T cells, animal models of arthritis, and applications of new technologies, such as Differential Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR), to define novel therapeutic targets. Both volumes combine to produce a concise set of protocols condensing decades of experience and expertise. ..Arthritis Research: Methods and Protocols will be a valuable tool for basic research
Pindex Book 2007
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發(fā)表于 2025-3-21 20:15:58 | 只看該作者
Hybridization of Synovial Tissuee, specific, and sensitive, because even small quantities of mRNA can be detected. Presented here contemporary protocols for ISH using a combined nonradioactive immunohistochemical detection system..In overview, the following steps have to be covered to perform ISH. (1) mRNA probes (sense and antise
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發(fā)表于 2025-3-22 00:54:14 | 只看該作者
Subtractive Hybridization is a screening method for differentially expressed genes. At first, poly-A.-RNA is isolated and reverse transcribed into cDNA. With the SMART technology, total RNA can be used. For subtractive hybridization, two adaptors are ligated to the tester cDNA. The tester cDNA is then mixed twice with cDNA
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發(fā)表于 2025-3-22 05:51:59 | 只看該作者
Laser Capture as a Tool for Analysis of Gene Expression in Inflamed Synoviumal biopsies. However, this strategy does not distinguish between specific gene expression profiles of cells originating from discrete tissue areas. Therefore, we established the combination of laser-mediated microdissection and RNA arbitrarily primed polymerase chain reaction (RAP-PCR) for different
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Image Analysis of Aggrecan Degradation in Articular Cartilage With Formalin-Fixed Samplesrtilage. The early histochemical/histological scale of Mankin (.) has been widely used but recently challenged as insufficient (.). Imaging techniques such as microscopic magnetic resonance imaging (MRI) (.), polarized light microscopy (.), atomic force microscopy (.), and infrared spectral analysis
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Measurement of Glycosaminoglycan Release from Cartilage Explantscy of different cytokines alone or in combination in simulating cartilage catabolism. Two approaches for GAG assay are described, with special attention being paid to the advantages and limitations of each method
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