Titlebook: Arabidopsis Protocols, 2nd Edition; Julio Salinas,Jose J. Sanchez-Serrano Book 2006Latest edition Humana Press 2006 DNA.Embryo.Genotyp.Mut

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Synchronization, Transformation, and Cryopreservation of Suspension-Cultured Cells is applicable both to . and the tobacco BY2 cell lines. Cell-cycle synchronization capacity of the parental lines is maintained after both transformation and recovery from cryopreservation. The techniques described here require no specialized equipment and are suitable for routine laboratory use, g

關(guān)心

Gene Identification and Cloning by Molecular Marker Mappingne fine-scale genetic linkage, ideally narrowing to a chromosomal region of about 40 kbp; and (4) sequencing of mutant and wild-type DNA is used to verify the identity of the mutated gene. Given a mutant phenotype that can be determined unambiguously in a single F2 plant, it is possible to complete

Manifest

PCR-Based Screening for Insertional Mutantsonce again, are becoming valuable for obtaining loss-of-function alleles in the genes “missed” by the sequencing projects. In this chapter, we provide a detailed description of the design and implementation of a PCR-based screen for insertional knockouts using T-DNA-mutagenized lines as an example.

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1064-3745 . Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the 978-1-61737-539-2978-1-59745-003-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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PALMY

勉勵(lì)

Cosmopolitan

monogamy

Empirische Forschung und Waldorfp?dagogikescribe standard cytogenetic methods including preparation of chromosomes and chromatin fibers, isolation of interphase nuclei, FISH and immunostaining, painting of . chromosomes, and comparative chromosome painting in other . species.