Titlebook: Apoptosis and Cancer; Methods and Protocol Gil Mor,Ayesha B. Alvero Book 2008 Humana Press 2008 Cancer research.Flow cytometry.Microarray.N

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https://doi.org/10.1007/978-3-663-20383-4borious techniques, caspase activity induction or inhibition measures are quantifiable and definitive. The highlighted techniques in this chapter are cost efficient and allow for the rapid exploration of thousands of combinations and conditions.

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Apoptotic Caspase Activation and Activity,robes allowed us to detect caspase activation in their working environment providing readout of the system with minimal interference. This chapter describes some of the methods used by our group to study the activation mechanisms of caspases and their activity.

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Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-Induced Apoptosis,e variety of apoptotic signals. Most common assays for examining caspase-3 activation include immunostaining, immunoblotting for active caspase-3, colorimetric assays using fluorochrome substrates, as well as employing the fluorescein-labeled CaspaTag pan-caspase in situ detection kit.

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Multiplex Caspase Activity and Cytotoxicity Assays,activity profiles within the same sample provides a level of normalization not possible with parallel assays. Furthermore, multiplexing caspase activity assays with viability and/or cytotoxicity assays can support conclusions regarding cytotoxic mechanism and provide normalization that may help correct for differences in cell number.

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