Titlebook: Antiviral Resistance in Plants; Methods and Protocol John M. Watson,Ming-Bo Wang Book 2012 Springer Science+Business Media, LLC 2012 Antivi

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Panos Panagiotopoulos,Frances Bowenng that leads to high-level virus resistance is triggered by virus-specific double-stranded (ds)RNA. The most efficient means to produce such dsRNA in transgenic plants is the introduction and expression of hairpin (hp) RNA constructs. Successful induction of the RNA silencing pathway is witnessed b

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Lecture Notes in Computer Science three methods for extracting small RNA from cells: a general method, one plant specific and a third particular to conifers. Further, protocols are given for the analysis and quantification of small RNAs using polyacrylamide gel-based approaches. A native streptavidin gel-shift assay, useful for mea

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Florian Evéquoz,Hugo Castanheirohas been associated with the synthesis of short interfering RNAs (siRNAs), which are essential regulators of genomic integrity and plant viral defense. The multiple gene copies, and functional diversities, of the plant RDRPs raise the question of whether their intrinsic properties differ. This chapt

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