Titlebook: Antifungal Drug Resistance; Methods and Protocol Damian J. Krysan,W. Scott Moye-Rowley Book 2023 The Editor(s) (if applicable) and The Auth

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Asseverate

Heterologous Expression of Candida Antifungal Target Genes in the Model Organism ecting resistance mechanisms. The influence of specific genes or gene alleles on a phenotype can initially be assessed within the model organism, .. . exhibits efficient rates of homologous recombination making it amendable for heterologous expression and represents a susceptible organism that can b

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maintenance

Transport across Membranes: Techniques for Measuring Efflux in Fungal Cellsux pumps or transporters. Drug efflux is a particularly common mechanism of resistance to azole antifungals, one of the most widely used classes of antifungal drugs. Here, we provide detailed protocols for two assays of small-molecule efflux activity: rhodamine 6G efflux and alanine-naphthylamide ac

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Normal-incidence waves in a stack of layers,gal isolate may be resistant to clinically available antifungals, and they are also amendable for research purposes, as the microtiter trays can be prepared in different formats with different antifungal and investigational agent concentration ranges and with different growth media if necessary. Thi

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Copy Number Variation and Allele Ratio Analysis in , Using Whole Genome Sequencing Data of CNVs and allele ratios utilizing free, open-source visualization tools. We provide code to use with an example dataset (matched antifungal drug-sensitive and drug-resistant . isolates) and notes on how to expand this protocol to other fungal genomes.