Titlebook: Antibody Usage in the Lab; Laura Caponi,Paola Migliorini Book 1999 Springer-Verlag Berlin Heidelberg 1999 Antibody.Antigen.ELISA.Immunoflu

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Antibody Specificity analyzed by Peptides synthesized on Cellulose Membranes,es have been developed for this purpose, including immunoblotting and ELISA (see Chapters 2 and 3), but one of the major limitations to the broader application of these techniques is the availability of synthetic peptides. In fact, while more and more laboratories now have their own automated peptid

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Book 1999he topics are discussed from a practical point of view, describing the theoretical basis of each technique and sample protocols plus a troubleshooting guide. Attention is focused on the various aspects of the protocols described thus providing the reader with the maximum possible information on each technique.

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https://doi.org/10.1007/978-3-8351-9240-9unoglobulins, usually protein A or protein G. Different preparations of unsolubilised protein A or G are commercially available. The matrix they are coupled to is usually sepharose, agarose or acrylic resins.

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,Elemente und Ger?te zur Energieübertragung,ght, being of different wavelengths, can be separated using optical filters. This is a sensitive technique since a positive signal is detected against a negative background. Furthermore, the ability to detect fluorescence simultaneously from two, three or four compounds fluorescing at different wavelengths allows multi-parametric analysis.

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Immunoblotting,lphate polyacrylamide electrophoresis, see Appendix 2), which is based on differences in the molecular weight of proteins, or isoelectrofocusing, which is based on differences in their isoelectric points. The separated antigens can then be transferred from the gel to a membrane for immunological characterisation.

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