Titlebook: Antibody Methods and Protocols; Gabriele Proetzel,Hilmar Ebersbach Book 2012 Springer Science+Business Media, LLC 2012 Antibodies.Antibody

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The Application of Transgenic Mice for Therapeutic Antibody Discovery,ion (FDA). Since then, a further seven such antibodies have been approved. In this chapter, we discuss how transgenic mice technologies can provide a powerful platform for creating human therapeutic antibodies.

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Production of Human or Humanized Antibodies in Mice,fined immunization protocols. Such an approach allows optimal in vivo affinity maturation of the humoral response. In addition, high-affinity antibodies arising in this context can readily be further characterized and produced as monoclonals after immortalizing and selecting specific antibody-produc

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Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format,v-Fc expression cassette into a mammalian expression vector followed by transient transfection of mammalian cells and purification by protein A affinity chromatography. The protocol is intended for applications in basic and preclinical research that require rapid access to milligram amounts of prote

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PEGylation of Antibody Fragments for Half-Life Extension,antages, including higher mobility and tissue penetration, ability to bind antigen monovalently and lack of fragment crystallizable (Fc) region-mediated functions such as antibody-dependent cell mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC). The main drawback for the use of

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