Titlebook: Antibody Engineering Protocols; Sudhir Paul Book 1995 Humana Press 1995

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Molecular Cloning of Antiground-State Proteolytic Antibody Fragments,biological functions of the constant regions. Rearrangement of variable-, diversity-, and joining-region genes and the hypermutability of the complementarity determining regions (CDRs) permit development of new antigen-binding specificities. With the discovery of natural catalytic activity in autoan

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1064-3745 Overview: 978-0-89603-275-0978-1-59259-538-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-3-658-10327-9ity immunoblotting (.) has proven to be a powerful and high-resolution method for estimating clonality of immune responses to a variety of antigens. This chapter describes the affinity immunoblotting method in detail.

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https://doi.org/10.1007/978-3-476-02810-5We have been collecting nucleotide and amino acid sequences of proteins of immunological interest, and aligning them in order to understand the structure and function relations of these proteins (.). To aid in organizing and analyzing this collection, a computer program, called SEQHUNT, was written.

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Konvexe Bewertungsringe und reelle Stellen,Antigen-binding sites in antibodies are formed by the variable regions of light (L) and heavy (H) chains. Delineation of antigen interactions with the individual subunits of antibodies is of interest for several reasons, including:

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Manfred Knebusch,Claus ScheidererMajor advances in the analysis of biomolecular structure have emerged from the development of highly specific monoclonal antibodies (MAb) by K?hler and Milstein in 1975 (.). MAb are produced from hybridoma cell lines that secrete a single species of antibody to a unique antigen. The advantages of MAb as reagents for antibody engineering are: