Titlebook: Animal Models of Allergic Disease; Methods and Protocol Kumi Nagamoto-Combs Book 2021 Springer Science+Business Media, LLC, part of Springe

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ECG769

Applications of Mouse Models to the Study of Food Allergy,odel and contemplated according to the intended purpose of the study. This chapter reviews our current knowledge on the application of mouse models to food allergy research and the variables that may influence the successful development of each type of model.

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Intravital Imaging of Vascular Permeability by Two-Photon Microscopy,tics of vascular permeability in vivo in real-time. This assay system provides a novel convenient way to study vascular biology that is beneficial in the assessment of various animal models of allergic disease.

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The Application of Flow Cytometry for Simultaneous and Multi-parametric Analysis of Heterogenous Cel phenotype and function. Here we describe a standardized protocol for surface and intracellular immune-phenotyping of murine lungs, beginning with the building of an optimal antibody panel and ending with data analysis and representation, including sample gating strategies for innate and adaptive immune responses.

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Assessment of Lung Eosinophils In Situ Using Immunohistological Staining,n paraffin-embedded lungs, as well as in cytospin preparations from the lung. These antibody staining techniques enable either colorimetric or fluorescence imaging of eosinophils or their granule proteins with the potential for additional antibodies to be added for detection of multiple molecules.

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Procedures to Evaluate Inflammatory and Pathological Changes During Allergic Airway Inflammation,ctural changes along with the measurement of important pro-inflammatory mediators such as Th1/Th2 cytokines and eotaxins. This chapter describes procedures to histologically evaluate inflammatory and pathological changes observed during allergic airway inflammation using lung tissue from mice.

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