Titlebook: Animal Coronaviruses; Leyi Wang Book 2016 Springer Science+Business Media New York 2016 Coronaviridae.Reverse genetics.Molecular diagnosti

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A Multiplex Polymerase Chain Reaction for Differential Detection of Turkey Coronavirus from Chicken tained for TCoV. In contrast, one PCR product of 356 bp corresponding to a fragment of N gene is obtained for IBV strains and one PCR product of 568 bp corresponding to a fragment of S gene is obtained for BCoV.

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1949-2448 ve, .Animal Coronaviruses. serves as an ideal reference for researchers examining a wide variety of coronavirus species in the .Coronaviridae..978-1-4939-8044-4978-1-4939-3414-0Series ISSN 1949-2448 Series E-ISSN 1949-2456

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Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissuesas alkaline phosphatase, also gives rise to significant advantages in defining the correlation of viral antigens with histopathologic lesions. PEDV antigens in frozen tissues are visualized as green staining in the cytoplasm of infected cells by fluorescent dyes conjugated with antibodies when activ

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An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypesostic laboratories. In this chapter, we describe a one-step RT-PCR which can be used for detecting most of IBV serotypes in the IBV-infected allantoic fluid and has been used routinely in our laboratories for detection of IBVs.

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Leyi WangIncludes cutting-edge methods and protocols for the study of coronaviruses in domestic animals and poultry.Provides step-by-step detail essential for reproducible results.Contains key notes and implem

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