Titlebook: Animal Cell Technology: Basic & Applied Aspects; Proceedings of the F H. Murakami,S. Shirahata,H. Tachibana Conference proceedings 1992 Spr

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Characterization of Cell Growth and Improvement of Attachment Kinetics on Macroporous Microcarriersused to “optically section” microcarriers and visualize the distribution of cells within them. In most microcarriers, cells were found to populate majority of the region close to the surface and some regions in the interior. However, a significant portion of the interior was not occupied. HepG2 cell

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Selective Formation and Immobilization by Re-Attachment of Floating Spheroids of Rat Hepatocytesf the cells have many clinical and industrial applications, including an artificial liver. Adult rat hepatocytes inoculated on positively-charged surfaces were reported to form spheroidal aggregates (spheroids), and they expressed higher hepatic functions in the longer term as compared to monolayer

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Engineering of Ultrafiltration Hollow Fiber Bioreactors for Mammalian Cell Protein Productiontion for months at high productivities. However, the heterogeneous nature of these bioreactors requires special considerations for proper design and operation. Downstream polarization of both cell growth and high molecular weight proteins occurs as a result of Starling flow in the immobilized cell r

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Production of hEPO by Recombinant C127 Cells using Hollow Fiber Culture Systemls in collagen gel. The EPO productivity in this improved system was six times as high as that without collagen gel. The properties of the extended cells after 5 months culture weren’t different from those of the master bank’s cells. It was shown that this immobilizing technology is suitable for the

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Oscillatory Behavior in Continuous Aggregate Cultures of Mammalian Cellsf settling is affected by the size of the aggregates. Using confocal microscopy and thin sectioning techniques, we have demonstrated that viability and other properties are distributed unevenly within the aggregates and are affected by the size of the aggregates. Thus, the variation in growth charac

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