Titlebook: Animal Cell Electroporation and Electrofusion Protocols; Jac A. Nickoloff Book 1995 Springer Science+Business Media New York 1995

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1064-3745 broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud- ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene st

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Gordian Schudt,Olga Dolnik,Stephan Beckerhat can be applied to eggs, sperm, platelets, mammalian cells, plant protoplasts, plant pollen, liposomes, bacteria, fungi, and yeast—generally to any vesicle surrounded by a membrane. The term “cells” will be used representatively for any of the vesicles to be manipulated unless specific requirements dictate otherwise.

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The Biology and Ecology of , Speciesr growth hormone. In most cases, transient-expression experiments have been used to study cellular processes, such as rat growth hormone gene expression (.), prolactin promotor activity (.–.), and the transcription regulatory properties of hormones, such as thyroid hormones and retinoic acid (.–.).

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https://doi.org/10.1007/978-3-319-18156-1respond to a particular enzymatic function, such as homologous recombination (.,.) or mismatch repair (.), can be introduced to determine whether a cell type of interest is positive or negative for that function.

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Electroporation of Rat Pituitary Cellsr growth hormone. In most cases, transient-expression experiments have been used to study cellular processes, such as rat growth hormone gene expression (.), prolactin promotor activity (.–.), and the transcription regulatory properties of hormones, such as thyroid hormones and retinoic acid (.–.).

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The Naming and Classification of , Species can readily be used for stable transfection experiments. However, before initiating an experiment, the life-span of the recipient cell strains should be characterized, and electroporation should not be performed on cells with insufficient proliferative potential remaining.

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The Biology and Ecology of , Speciesapparatus and are technically difficult, whereas others, such as protoplast fusion, retroviral vectors, and liposome fusion, require time-consuming biochemical manipulations. Electroporation, which circumvents most of these problems, has emerged as an effective tool for the transfection of eukaryotic cells in suspensions (for a review, . ref. .).