Titlebook: Animal Cell Culture Techniques; Martin Clynes Book 1998 Springer-Verlag Berlin Heidelberg 1998 Colon.Monoclonal Antibodies.cell.cell cultu

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Assessing Release of Secretory Products from Individual Cellsg modifications of the original hemolytic plaque assay. This assay was developed for evaluating Ig production by lymphocytes (Jerne et al. 1974), and the modifications, referred to as reverse hemolytic plaque assays (RHPAs), are based on the complement-induced lysis of red blood cells (RBCs) bearing

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SMART

Investigations into Cell Differentiation Using Cells in Culturet stem cell, having the potential to form a complex organism through proliferation, differentiation and organization of the differentiated tissue. Cellular differentiation may be defined as the process by which “a cell acquires or displays a new stable phenotype without changing its genotype” (Ham a

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The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammare arrested at different stages of myeloid development. These lines have been widely studied as models of myeloid differentiation and as a substitute for their more mature, normal myeloid counterparts which sometimes are cumbersome to use. The most broadly studied human myeloid cell line, the human

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Generation of Monoclonal Antibodies and Characterization of Novel Antibodies by Western Blotting and range of antigens have been produced. Monclonal antibodies have many advantages over polyclonal antibodies, e.g., their specificity of binding, their homogeneity (antibody produced by all the decendants of one hybridoma cell is identical) and their ability to be produced in unlimited quantities (as

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Methods for Studying Phosphoinositide Metabolism in Cultured CellsG), which activates protein kinase C (PKC) (Fig. 10.1). These second messengers are responsible for mediating the intracellular consequences of first messengers (e.g., hormones, growth factors, neurotransmitters, agonists; i.e., extracellular molecules) binding to the plasma membrane. These two mess

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The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and InflammML with maturation, FAB-M2 (Dalton et al. 1988). APL represents a model for the therapeutic approach of differentiation therapy, as APL cells are able to respond to all-. retinoic acid (RA) treatment by terminal differentiation (Huang et al. 1988). The chromosomal translocation t(15;17) in the blast