Titlebook: Animal Cell Culture; John M. Walker,Jeffrey W. Pollard,John M. Walker Book 1990 Springer Science+Business Media New York 1990

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https://doi.org/10.1007/978-1-4613-8722-0ans, current theories are that B lymphocytes differentiate in the fetal liver and in the bone marrow of adults. Human T and B cells are most easily obtained either from peripheral blood or from biopsy of lymphoid tissues (lymph nodes, spleen, Peyer’s patches from gut, tonsils, and adenoids).

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Eating Disorders in Boys and Meneover, the stem cells from which muscle develops in tissue culture seem to be very resilient; they will survive anoxic conditions within muscle at temperatures between 4 and 37°C, for up to a few days, and when obtained in suspension, are easy to freeze (with 10% DMSO as a cryoprotectant) and store in liquid nitrogen.

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Separation and Maintenance of Primary T and B Lymphocytes,ans, current theories are that B lymphocytes differentiate in the fetal liver and in the bone marrow of adults. Human T and B cells are most easily obtained either from peripheral blood or from biopsy of lymphoid tissues (lymph nodes, spleen, Peyer’s patches from gut, tonsils, and adenoids).

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Establishment of Lymphoblastoid Cell Lines,s the purpose of this chapter to provide all the information necessary to run an efficient and safe Epstein-Barr Virus (EBV) transformation system. More detailed information regarding the use of this technique for the preparation of human monoclonal antibodies is given elsewhere (.).

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