Titlebook: Animal Cell Culture; Mohamed Al-Rubeai Book 2015 Springer International Publishing Switzerland 2015 Animal Cell Culture.Biologics.Biopharm

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Monitoring of Cell Culture, which extensive monitoring is a prerequisite for. In this chapter, the current state of monitoring technologies and applications is reviewed. Particular emphasis is placed on biological parameters, i.e. cell density and viability as well as substrates, metabolites, and product concentration but als

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Glycosylation in Cell Culture,allowed linking specific glycan structures to functionality of glycoproteins, which have led to efforts to modify glycosylation through genetic engineering, sequence-interfering RNA (siRNA), glycosylation inhibitors or chemoenzymatic modification.

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Mammalian Cell Line Selection Strategies for High-Producers,nd determine clonality of a cell line expressing a quality biotherapeutic protein at the highest specific productivity possible. However, challenges remain in dealing with the sheer volume of cells that need to go through the screening process for the determination of a stable highly productive clon

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Metabolic Flux Analysis: A Powerful Tool in Animal Cell Culture,erall trends, supplemented with relevant examples in several animal cell systems. A general picture on the subject will be depicted aiming at the design and development of new techniques for tackling prevailing challenges that need to be overcome in biopharmaceuticals production, biomedical research

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,Proteomics in Cell Culture: From Genomics to Combined ‘Omics for Cell Line Engineering and Bioproceerves to identify and quantify protein levels. Optimizations of sample preparations and database development, including a detailed CHO proteome now available, have improved the quantity and accuracy of identified proteins. The applications are widespread and expanding, thus suggesting numerous appli