Titlebook: Analytics of Protein-DNA Interactions; Harald Seitz Book 2007 Springer-Verlag Berlin Heidelberg 2007 Biochemistry.Biotechnology.DNA.Microa

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Chahrazed Ksouri,Abdelfettah Belghithce specificities of TFs, other DNA binding proteins, or synthetic compounds. DNA bindingsite data from PBMs combined with gene annotation data, comparative sequence analysis, and gene expressionprofiling, can be used to predict what genes are regulated by a?given TF, what the functions are ofa?given

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Monitoring Methylation Changes in Cancer,de polymorphism. Eventhough different approaches have been established for analysing DNA methylation, so far detection methodsthat are capable of surveying the methylation status of multiple gene promoters have been restricted toa?limited number of cytosines. The use of oligonucleotide microarrays p

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transplantation

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,Protein Binding Microarrays for the Characterization of DNA–Protein Interactions,ce specificities of TFs, other DNA binding proteins, or synthetic compounds. DNA bindingsite data from PBMs combined with gene annotation data, comparative sequence analysis, and gene expressionprofiling, can be used to predict what genes are regulated by a?given TF, what the functions are ofa?given

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Identification and Characterization of DNA-Binding Proteins by Mass Spectrometry,epertoire of mass spectrometric methods that arenow available for the identification and detailed characterization of DNA-binding proteins. These techniques,how they work, what their requirements and limitations are, and selected examples that document their performanceare described and discussed in

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https://doi.org/10.1007/978-3-030-99004-6 feature, enabling relative affinities to be calculated. Keyfactors for reproducible and accurate quantification of protein binding are: microarray surface chemistry;length of oligonucleotides; position of the binding site sequence; quality of the protein and antibodies;and hybridisation conditions.

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