Titlebook: All-Optical Methods to Study Neuronal Function; Eirini Papagiakoumou Book‘‘‘‘‘‘‘‘ 2023 The Editor(s) (if applicable) and The Author(s) 202

糾纏

基因組

細胞

An All-Optical Physiology Pipeline Toward Highly Specific and Artifact-Free Circuit Mapping,or co-expression, and tailored illumination schemes. It furthermore demands concepts for system integration and a dedicated analysis pipeline for calcium transients in an event-related manner. Here, firstly, we put forward a framework for the specific requirements for technical system integration pa

遍及

努力趕上

Miniature Multiphoton Microscopes for Recording Neural Activity in Freely Moving Animals,earch. In combination with fluorescent reporters, these miniature microscopes allow researchers to record the neural activity that underlies behavior, cognition, and perception in freely moving animals. Single-photon miniature microscopes are convenient for widefield recording but lack the increased

myocardium

Optogenetics and Light-Sheet Microscopy,ge field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in combination with light-sheet microscopy and discuss some current bottlenecks and horizons of light sheet in all-opt

一再困擾

Inveterate

直覺沒有

Optical and Analytical Methods to Visualize and Manipulate Cortical Ensembles and Behavior,haracterization of causal relations between neuronal activity and behavioral states. In this chapter, we review the implementation of simultaneous two-photon imaging and holographic optogenetics in conjunction with population analytical tools to identify and reactivate neuronal ensembles to control

KIN

Illuminating Neural Computation Using Precision Optogenetics-Controlled Synthetic Perception, measures. In this chapter, we present an overview of current methods for connecting neural codes to perception using precision optogenetics and psychophysical measurements of synthetically induced percepts. We also highlight new methodologies for validating precise control of optical and behavioral