Titlebook: Agrobacterium Protocols; Volume 2 Kan Wang Book 2015Latest edition Springer Science+Business Media New York 2015 Agrobacterium.Agrobacteriu

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Dao Companions to Chinese Philosophy with . strain EHA105 harboring pBI121 plant binary vector, which contains . as plant selectable marker and . as reporter. The putative transformed plants are selected on kanamycin, and stable integration of transgene(s) is confirmed by histochemical GUS assay, polymerase chain reaction, and Southern hybridization.

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Cassava (, Crantz) binary expression cassette is used to transform this tissue, which is regenerated via cotyledons and shoot organogenesis to produce rooted in vitro plantlets. This chapter details each step of the procedure using the model cultivar 60444 and provides supplementary notes to successfully produce transgenic cassava.

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