Titlebook: Agrobacterium Protocols; Volume 1 Kan Wang Book 2015Latest edition Springer Science+Business Media New York 2015 Agrobacterium-mediated tra

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Interest in plant model systems for genetic, methods for .-mediated transformation of seed-derived callus. Vectors used for gene constructs contained the hygromycin phosphotransferase gene as a selectable marker. The transition of transgenic lines to soil was straightforward; plants started to flower in 1–3 weeks, with seeds ready to harvest approximately 5 weeks later.

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Transformation Using Cotyledonary Explants is difficult to regenerate from callus cultures. By using cotyledons as explants for . infection and direct shoot formation, this protocol allows for rapid production of transgenic plants from Jemalong A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse i

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Interest in plant model systems for genetic, n time, small stature, and simple growth requirements. A model species would be especially advantageous for the studies of C. photosynthetic grasses, which currently present practical challenges. These include long seed-to-seed generation times, and because of their large size at maturity they requi

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