Titlebook: Agrobacterium Protocols; Volume I Kan Wang Book 2006Latest edition Humana Press 2006 Agrobacterium tumefaciens.Arabidopsis thaliana.DNA.Flo

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書目名稱Agrobacterium Protocols影響因子(影響力)




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書目名稱Agrobacterium Protocols網(wǎng)絡(luò)公開度




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書目名稱Agrobacterium Protocols被引頻次學(xué)科排名




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書目名稱Agrobacterium Protocols讀者反饋




書目名稱Agrobacterium Protocols讀者反饋學(xué)科排名

嫌惡

Ringworm

Three Methods for the Introduction of Foreign DNA into ,ormable plant cells through the use of T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into . using the triparental ma

杠桿支點(diǎn)

認(rèn)識(shí)

Nucleic Acid Extraction from , Strainsells often requires the manipulation and analysis of nucleic acids present in recombinant . strains. Here we present dependable methods for the isolation of genomic (total) DNA, mega-plasmid DNA, shuttle or binary plasmid DNA, and RNA. In addition, we provide a simple method for the electronic trans

教唆

Virulence Gene Inductionion as a two-component regulatory system to sense particular phenolic compounds synthesized by wounded plant tissues. Induction by these phenolic compounds, in the presence of certain neutral or acid sugars, results in activation of other . genes, leading to the processing of T-DNA from the Ti-plasm

謙卑

Floral Dip Transformation Methodsequently set seed, and transgenic plants are then selected among the progeny seedlings. Because no plant tissue culture is required, somaclonal variation is avoided, and the procedure can be performed easily by nonspecialists. Success rates are high: it is common that 1% of the progeny seedlings ar

情節(jié)劇

滑稽

Transformation Using Cotyledon Explantse genotype Jemalong A17 is in progress. By using cotyledons as explants for . infection and direct shoot formation, this protocol allows for rapid production of transgenic plants from A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse in only 3-4 mo after

善于騙人

Transformation Using Root Explantstions, including nodulation and mycorrhizal colonization. The development of different transformation methods is an important aspect for functional genomic studies in the species. This protocol describes an efficient system for generating transgenic plants from . roots based on .-mediated transforma