Titlebook: Aging; Methods and Protocol Sean P. Curran Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Drosophila.Yeast.li

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Methods for Assessing Fertility in , from a Single Population,, producing a defined number of sperm during larval development before switching exclusively to oogenesis. Here we show a method of assaying both oocyte and sperm quality from a single population of animals.

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,Staining and Quantification of β-Amyloid Pathology in Transgenic Mouse Models of Alzheimer’s Diseasaccomplished through both biochemical and histochemical approaches. In this chapter, we describe two techniques for the histological detection of Aβ, immunostaining with Aβ antibodies and staining with the amyloid dye thioflavin S, and its quantification using digital imaging.

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Metabolic Assessment of Lipid Abundance and Distribution,e in lipid homeostasis due to its short lifespan and remarkably well-conserved metabolic pathways. Due to their ease of use and similarities, there have been numerous developments in methodologies to study intracellular lipid abundance and tissue distribution in ..

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,Quantitative Profiling of Lipid Species in , with Gas Chromatography–Mass Spectrometry, well as a method that quantitates fatty acids following separation into neutral lipid pools (triacylglycerols and cholesteryl esters) versus more polar lipids (phospholipids) by solid-phase extraction (SPE).

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Book 2020eir respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...?Authoritative and cutting-edge,? .Aging: Methods and Protocols .aims to ensure successful results in the further study of this vital field..