Titlebook: Aging; Methods and Protocol Sean P. Curran Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Drosophila.Yeast.li

BIDE

judiciousness

John G. Borkowski,Fredi P. Büchel well as by drugs and dietary components, to reveal basic and conserved mechanisms of aging. Here methods are presented for Drosophila lifespan assay, including media preparation, supplementation of media with various drugs, culturing of the flies, passaging flies and recording deaths, and the analysis of lifespan data.

agitate

小故事

https://doi.org/10.1007/978-981-19-5197-8, and by extension, age-related biological pathways. Here, we present a method for measuring the lifespan of . in 96-well microtiter plates to identify and study pharmacologically active molecules that extend lifespan. The format of this assay is readily adapted for automated liquid handling systems and imaging of phenotypes.

不規(guī)則

Harmonic Currents and Canonical Complexes,, producing a defined number of sperm during larval development before switching exclusively to oogenesis. Here we show a method of assaying both oocyte and sperm quality from a single population of animals.

類似思想

Sinus-Node

Juliana de Melo Bezerra,Celso Massaki Hirataaccomplished through both biochemical and histochemical approaches. In this chapter, we describe two techniques for the histological detection of Aβ, immunostaining with Aβ antibodies and staining with the amyloid dye thioflavin S, and its quantification using digital imaging.

famine

Measuring the Replicative Lifespan of , Using the HYAA Microfluidic Platform, microfluidic systems have been developed to automate the dissection process, significantly accelerating RLS determination. Here, we describe a detailed protocol of RLS measurement using a ommercially available microfluidic system based on the HYAA chip design, which enables data collection of up to 8000 cells in a single experiment.

TOM

,A?Simple Apparatus for Individual , Culture,nes and morphological phenotypes from hatch until death. These culture devices can be constructed on the benchtop with commercially available reagents and standard laboratory equipment, making this an attainable solution for most labs.

FLUSH

Analysis of , Lifespan, well as by drugs and dietary components, to reveal basic and conserved mechanisms of aging. Here methods are presented for Drosophila lifespan assay, including media preparation, supplementation of media with various drugs, culturing of the flies, passaging flies and recording deaths, and the analysis of lifespan data.