Titlebook: Advances in New Technology for Targeted Modification of Plant Genomes; Feng Zhang,Holger Puchta,James G. Thomson Book 2015 Springer Scienc

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Developing CRISPR Technology in Major Crop Plants,cently, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system-based RNA-guided endonucleases have been added to the SSN toolbox. TGMs generated by this system rely on a synthetic single guide RNA (sgRNA) to direct the Cas9 protein to cleave a predetermined

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