Titlebook: Advanced Photon Counting; Applications, Method Peter Kapusta,Michael Wahl,Rainer Erdmann Book 2015 Springer International Publishing Switze

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https://doi.org/10.1007/978-3-030-63982-2he microsecond timescale. Showing examples including the study of a biological macromolecule, we demonstrate the usefulness of these two methods in real applications. In addition, we present another application of time-tagged TCSPC, which analyzes photon interval time for characterizing timing insta

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https://doi.org/10.1007/978-3-319-24987-2ifetime imaging microscopy (FLIM) detects the time duration of fluorescence emission, taking advantage of the multidimensional nature of photon emission. FLIM-based intracellular sensing approaches, especially in the time domain in single-photon timing (SPT) mode, overcome many of the limitations of

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Vladimir A. Alekseenko,Alexey V. Alekseenkowith bursts of closely spaced laser excitation pulses for excitation (multi-pulse excitation) that allows for many-fold increase in the intensity of a long-lived probe over the background signal. This technology can be easily implemented for biomedical diagnostics and imaging to significantly enhanc

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https://doi.org/10.1007/978-3-031-40470-2ty by combining STED laser operating in CW with pulsed excitation and time-gated photon detection. Here, we describe the physical principles of gCW-STED, formulate the theoretical framework which characterizes its main benefits and limitations, as well as show experimental data.

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Robert E. M. Hedges,Gert J. Van Klinkene-resolved measurements to monitor breast tumor degeneration by neoadjuvant chemotherapy is discussed. Finally, fluorescence mammography with the contrast agent indocyanine green is considered as a tool to improve differentiation between malignant and benign breast lesions.

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Advanced FCS: An Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual-Focus FCS,