Titlebook: Adenovirus Methods and Protocols; Volume 2: Ad Protein William S. M. Wold,Ann E. Tollefson Book 2007Latest edition Humana Press 2007 PCR.RN

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Bat Evolution, Ecology, and Conservationnces in the packaging domain contribute to viral DNA encapsidation, and several viral proteins bind to these repeats when analyzed using in vitro DNAprotein-binding assays. This chapter describes a chromatin immunoprecipitation approach to study the binding of viral proteins to packaging sequences i

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Georgina Ross,Marc W. Holderiedection of the in vivo interaction of specific proteins with specific genomic regions. In this technique, living cells are treated with formaldehyde to crosslink neighboring proteinprotein and proteinDNA molecules. The crosslink with formaldehyde is reversible and covers a short distance (2 ?); the c

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Bat Evolution, Ecology, and ConservationWe were particularly interested in identifying cellular proteins that interact with fiber knob, which is the moiety within the Ad capsid responsible for high-affinity attachment of virus to cellular receptors. We used recombinant fiber knob domains from members of group C and B Ads to purify virus i

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Bat Evolution, Ecology, and Conservation, a controlled amount (ranging from several to many thousands of copies) of a viral or cellular gene, a protein product of a gene, a polypeptide fragment encoding a specific protein domain, or an RNA molecule can be delivered into a target cell and the functional consequences analyzed. Microinjectio

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A. Raich,X. Wu,H.-F. Lin,Ali Cinarhumans or NK1.1 antigen in certain strains of mice. NK cells display spontaneous lytic activity but do not exhibit immunological memory. NK cells are important mediators of antiviral and antitumor immunity. Standard NK cytolysis assays measure the ability of NK cells to kill certain target cells (tu

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Hermann Mitterhofer,Pia Andreattanactive monomer, which is activated during virus maturation by an 11-residue cleavage fragment of capsid protein pVI. This chapter describes the synthesis, purification, activation, and assay of recombinant human Ad type adenain.