Titlebook: Adeno-Associated Virus Vectors; Design and Delivery Michael J. Castle Book 2019 Springer Science+Business Media, LLC, part of Springer Natu

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Springer Science+Business Media, LLC, part of Springer Nature 2019

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Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/144869.jpg

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Volker Arolt,Christian Reimer,Horst Dillingthe contact residues on the AAV capsid and covalently coupling targeting ligands to the capsid surface that exhibit high affinity for a cell surface protein of choice. This way, selective gene delivery to target-receptor positive cell types has been achieved. Two methods for coupling targeting ligan

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Volker Arolt,Christian Reimer,Horst Dillingr ex vivo gene transfer. Its applications range from a tool for experimental purposes to preclinical and clinical gene therapy. The ability to accurately and reproducibly quantify vector concentration is critical for any of these applications. While several quantification assays are available, here

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Basiswissen Psychiatrie und Psychotherapierization of process- and product- related impurities found in rAAV stocks in order to assess the potential risks for patients. During production, rAAV capsids are known to internalize illegitimate DNA fragments in addition to their recombinant genome. These contaminants can come from plasmid or help

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Volker Arolt,Christian Reimer,Horst Dillingn (ISH) uses synthesized complementary RNA or DNA nucleotide probes to localize and detect sequences of interest in fixed cells, tissue sections, or whole tissue mounts. Variations in techniques include adding labels to probes, such as fluorophores, which can allow for the simultaneous visualization

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Basiswissen Psychiatrie und Psychotherapient. With the rapid advancement of the CRISPR/Cas9 genome editing technology, in vivo application of CRISPR/Cas9 holds immense potential for treatment of these diseases. Adeno-associated virus (AAV) vectors are an ideal gene transfer tool for delivery of CRISPR components to the retina. Here, we desc