Titlebook: Activity-Based Proteomics; Methods and Protocol Herman S. Overkleeft,Bogdan I. Florea Book 2017 Springer Science+Business Media New York 20

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https://doi.org/10.1007/978-3-531-91576-0rometry is the primary method for target identification, and in the last decade, cleavable linkers have become a popular strategy to facilitate protein enrichment and identification. In this chapter, we provide an overview of cleavable linkers used in chemical proteomics approaches, discuss their di

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https://doi.org/10.1007/978-1-4939-6439-0activity-based proteomics; annotate enzyme families; activity-based protein; bioorthogonal chemistry; an

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978-1-4939-8200-4Springer Science+Business Media New York 2017

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Activity-Based Proteomics978-1-4939-6439-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Nils Labahn,Fabian Uterm?hlen,Harry Weberocol, we describe the workflow for an activity-based protein profiling (ABPP) experiment with an alkyne-tagged natural product derivative. Our protocol includes experimental procedures for in vivo labeling, sample preparation and 2-step (click chemistry) visualization and sample preparation for mass spectrometry-based target identification.

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Book 2017vity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily repro

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