Titlebook: A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis; Thomas Meier,Falk Fahrenholz Book 1996 Birkh?user Verlag 1996 DNA.PAC.PCR.S

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How Set Theory Impinges on Logic Terminal sequences from YACs are isolated to verify if the clone is nonchimeric and to generate sequence-tagged sites for mapping and isolation of overlapping clones. These end sequences can be selectively amplified by Alu-vector polymerase chain reaction (PCR); this method uses a primer correspond

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978-3-0348-7351-2Birkh?user Verlag 1996

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Perspectives in Vertebrate Scienceraction, this assay enables a more accurate determination of the lectin bound to the ligand-coated plate than an antibody-mediated, enzyme-linked immunosorbent assay (ELISA). The application of biotinylated lectins for detection of glycoproteins on the blot is also described and illustrated with several examples.

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Alternative Logics. Do Sciences Need Them? biotin-labeled probes by polymerase chain reaction (PCR) is included. The technical difficulties encountered with densitometric signal quantitation of nonradioactive blots are discussed, and procedures for relative and absolute quantitation of bound probe are presented.

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https://doi.org/10.1007/978-3-031-13471-5 the bound antibodies were eluted by photocleavage of the affinity ligand. The eluted antibodies showed essentially unchanged binding characteristics. The approach may prove to be generally useful in the isolation of labile proteins in an intact form.

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An Introduction to Avidin-Biotin Technology and Options for Biotinylation,eral servo manipulators 155 Special characteristics of teleoperators 158 Design criteria for teleoperators 159 Vehicles and transporters 160 Applications of teleoperators 161 Remote handling of radioactive materials 161 Remote handling of explosive and toxic materials 161 Telemanipulation of heavy o