Titlebook: Working with Stem Cells; Henning Ulrich,Priscilla Davidson Negraes Book 2016 Springer International Publishing Switzerland 2016 Nanostruct

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Motor Neuron Differentiation from Pluripotent Stem Cells: Development of the Technique, Synopsis ofOver the last decade, the motor neuron disease field has made incredible strides by establishing efficient and reliable protocols for generating motor neurons . from pluripotent stem cells. In this chapter, we will consider the biology of motor neuron development, describe protocols for generating m

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Pancreatic Differentiation from Human Pluripotent Stem Cells,re of hPSCs to different signaling factors, thereby moving cells through several developmental intermediates towards the pancreatic fate. Upon implantation into mice, hPSC-derived pancreatic progenitor cells spontaneously differentiate into endocrine and exocrine cells. Here, we describe a detailed

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Efficient Generation of Skeletal Myogenic Progenitors from Human Pluripotent Stem Cells,published protocol for deriving skeletal myogenic progenitors from these cells. This method takes advantage of the ability of the GSK3β inhibitor to induce paraxial mesoderm commitment in differentiating cells. In addition, upon flow cytometry purification, human PAX7-induced skeletal myogenic proge

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Genome Editing in Stem Cells, captivates the attention of scientists and is crucial to elucidate genetic function. Now, scientists can emulate monogenic diseases to study pathogenesis on the molecular and cellular levels. In addition, medical personnel could revert a deleterious mutation to its wild-type form as a potential gen

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Simone Aparecida Siqueira Fonseca,Roberta Montero Costas,Lygia V. Pereira