Titlebook: Whole Genome Amplification; Methods and Protocol Thomas Kroneis Book 2015 Springer Science+Business Media New York 2015 Circulating Tumor C

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The Single-Cell Lab or How to Perform Single-Cell Molecular Analysis,le cells. PCR-based amplification techniques are widely used in this field. However, setting up an experiment and analyzing the results can sometimes be challenging. The aim of this chapter is to provide a general overview on single-cell PCR analysis focusing on the potential pitfalls and on the pos

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Sample Preparation Methods Following CellSearch Approach Compatible of Single-Cell Whole-Genome Amp using single cells is to isolate these cells from the surrounding cells, especially when the targeted cells are rare. A number of techniques have been developed for this goal, each having specific limitations and possibilities. In this chapter, five of these techniques are discussed in the light of

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Deterministic Whole-Genome Amplification of Single Cells,e Genomic Hybridization (SCOMP)” (Klein et al., Proc Natl Acad Sci U S A 96(8):4494–4499, 1999). The method has recently become available commercially under the name “.1. WGA Kit.” It is a PCR-based technique for whole genome amplification (WGA) allowing comprehensive and quite uniform amplification

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,Heat-Induced Fragmentation and Adapter-Assisted Whole Genome Amplification Using GenomePlex? Singlechnique described here is based on heat-induced random fragmentation yielding DNA strands mainly ranging from 0.1 to 1 kb in length. The fragmented DNA is then subjected to library generation by annealing of adaptor sequences to both ends of the DNA fragments. Using primers hybridizing to the adapte

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Using Multiplex PCR for Assessing the Quality of Whole Genome Amplified DNA,d from heat-induced random fragmented DNA. A set of four primer pairs is used to amplify DNA sequences of WGA products in and downstream of GAPDH gene in yielding 100, 200, 300, and 400 bp fragments. PCR products are analyzed by agarose gel electrophoresis and the respective WGA quality is classifie

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,Laser Microdissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by ,1?,ue sections. In this chapter, we describe a workflow for microdissecting small regions of interest from cancer tissue, i.e. formalin-fixed paraffin-embedded (FFPE) and cryo-conserved specimens, and subsequent whole genome amplification by a deterministic WGA approach (.1? WGA).

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