Titlebook: Wheat Rust Diseases; Methods and Protocol Sambasivam Periyannan Book 2017 Springer Science+Business Media LLC 2017 PgtSNP chip.KASPer.cloni

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Race Typing of , on Wheatred fluid, Novec? 7100, is presented. Differential sets consisting of near-isogenic Avocet lines, selected lines from the “World” and “European” sets, and additional varieties showing race-specificity facilitate a robust assessment of race, irrespectively of geographical and evolutionary origin of i

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Extraction of High Molecular Weight DNA from Fungal Rust Spores for Long Read Sequencingsexual infection cycle on wheat, rusts produce massive amounts of dikaryotic urediniospores. These spores are dikaryotic (two nuclei) with each nucleus containing one haploid genome. This dikaryotic state is likely to contribute to their evolutionary success, making them some of the major wheat path

用肘

Microsatellite Genotyping of the Wheat Yellow Rust Pathogen ,markers, particularly microsatellites, have been reported to be important tools for deciphering pathogen population structure, invasion sources, and migration history. The utility of these DNA-based markers and sequencing has been increased by the direct DNA extraction from infected leaves with subs

FLASK

Computational Methods for Predicting Effectors in Rust Pathogensexpression profiles during early and late infection stages. However, the set of secreted proteins expressed during infection is too large for experimental investigations and contains not only effectors but also proteins that play a role in niche colonization or in fighting off competing microbes. Th

PACK

Protein–Protein Interaction Assays with Effector–GFP Fusions in ,ctor biology is to determine the host cell compartments and the protein complexes in which effectors accumulate. Here, we describe a five-step pipeline that we routinely use in our lab to achieve this goal, which consists of (1) Golden Gate assembly of pathogen effector–green fluorescent protein (GF

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Apoplastic Sugar Extraction and Quantification from Wheat Leaves Infected with Biotrophic Fungiom the cytoplasm of host cells and/or the apoplastic spaces. Uptake of nutrients from the cytoplasm is via intracellular hyphae or more complex structures such as haustoria. Apoplastic nutrients are taken up by intercellular hyphae. Overall the infection creates a sink causing remobilization of nutr