Titlebook: New Aspects in Analyzing the Synaptic Organization of the Brain; Joachim H.R. Lübke,Astrid Rollenhagen Book 2024 The Editor(s) (if applica

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Large-Volume Electron Microscopycantly during the past two decades. These methods include focused ion beam (FIB)-scanning electron microscopy (SEM), serial block face (SBF)-SEM, automated tape-collecting ultramicrotome (ATUM)-SEM, and ATUM-transmission electron microscopy (TEM). Here, an outline of these imaging technologies and t

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Multiplexed ,STORM Imaging of Neuronal Tissue and Cellshe highly demanding processes of synaptic transmission. Most classical single-molecule localization microscopy (SMLM) methods allow the visualization and identification of molecular nanostructures at approximately 20–30?nm lateral and 60?nm axial resolution but are often limited to simultaneous acqu

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Detailed Phenotyping of the Physiology, Structure, and Connectivity of Individual Neocortical Neuronmalian brain. First, a brief overview is provided of widely used approaches to study the intrinsic properties of neurons, including their morphology, physiology, and transcriptomic profile. Next, we describe in detail multi-modal methods that we and others have developed and employ to quantify both

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3D Quantitative Analysis of Synaptic Ultrastructure in Identified Long-Range Projection Axonse of these synapses is important to model interactions between the thalamus and cortex..Specifically, we chose the ventral posteromedial (VPM) and posterior (Po) thalamic nucleus axons that both converge in different layer patterns in the primary and secondary somatosensory cortex. Here, we set out

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Counting Synaptic Vesicles: As Simple as That?ionally defined pools of synaptic vesicles (SVs) in particular that of the so-called readily releasable pool of SVs (RRP). However, due to the random distribution of SVs within a given synaptic bouton, it is impossible to describe a structural correlate to the functionally defined pools. Thus, the s

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