標(biāo)題: Titlebook: Vertebrate Embryogenesis; Embryological, Cellu Francisco J. Pelegri Book 2019Latest edition Springer Science+Business Media, LLC, part of S [打印本頁(yè)] 作者: digestive-tract 時(shí)間: 2025-3-21 17:22
書(shū)目名稱(chēng)Vertebrate Embryogenesis影響因子(影響力)
作者: 小鹿 時(shí)間: 2025-3-22 00:16
Methods in Molecular Biologyhttp://image.papertrans.cn/v/image/982302.jpg作者: 男生戴手銬 時(shí)間: 2025-3-22 00:35
https://doi.org/10.1007/978-1-4939-9009-2Xenopus; Zebrafish; Balbiani body; Mouse embryos; CRISPR-Cas9; Model systems; Non-traditional species作者: 是比賽 時(shí)間: 2025-3-22 07:27
Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections,RNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.作者: 手工藝品 時(shí)間: 2025-3-22 09:39 作者: sed-rate 時(shí)間: 2025-3-22 14:00 作者: Oration 時(shí)間: 2025-3-22 17:11
Generation of , Haploid, Triploid, and Hybrid Embryos,genetic relationships. Here we describe methods to generate haploid, triploid, and hybrid species starting from eggs and sperm of . and . that enable investigation of how genome size and content affect physiology at the organismal, cellular, and subcellular levels.作者: Substitution 時(shí)間: 2025-3-22 23:24 作者: 慢跑鞋 時(shí)間: 2025-3-23 02:05
Vertebrate Embryogenesis978-1-4939-9009-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 抵押貸款 時(shí)間: 2025-3-23 06:57 作者: 清醒 時(shí)間: 2025-3-23 13:39
Edwina McGlinn,Miriam A. Holzman,Jennifer H. Mansfield作者: 圍巾 時(shí)間: 2025-3-23 13:58 作者: paragon 時(shí)間: 2025-3-23 18:22 作者: Gourmet 時(shí)間: 2025-3-23 23:46
Michael D. Sheets- hochschulen als studienbegleitende Grundlage gedacht. Wir hoffen aber, da? auch Fach- leute aus dem Bereich des Bauingenieurwesens und der Architektur hier wesentliche Informationen und Anregungen finden, die sie in ihrer beruflichen Arbeit unterstützen. Die Zielsetzung eines m?glichst weitgreifen作者: 熱心 時(shí)間: 2025-3-24 03:26
Laura S. Gammill,Bridget Jacques-Fricke,Julaine Roffers-Agarwalemark from the .: ‘Sentiment de ., dès mon enfance. Malgré la famille, — et au milieu des camarades, surtout, — sentiment de destinée éternellement solitaire’s we can well imagine how Baudelaire responded to De Quincey’s conclusion to .: ‘Oh, burden of solitude, that cleaves t to man through every s作者: 合唱團(tuán) 時(shí)間: 2025-3-24 06:31
Matthew K. Vickaryous,Emily A. B. Gilberterte positive Raumladung auf. Im P-Gebiet verbleiben unkompensierte negative Akzeptoren und zus?tzlich eingedrungene Elektronen, so da? dort eine negative unkompensierte Raumladung entsteht (Bild ..1 c). Diese Raumladung baut ein elektrisches Feld auf, das einen zum Diffusionsstrom der Majorit?tstr?作者: 整體 時(shí)間: 2025-3-24 11:59
Amanda Butler,Dawn Owens,Mary Lou King,Tristan Agueroengeh?use für eine Xenonlampe, die mit zwei Blattfedern an einer Asbestschieferplatte befestigt ist. Mit der isolierten Schraube im Deckel l??t sich die Lampe in der H?he verstellen, seitlich mit den links oben sitzenden Schrauben nach dem Prinzip Abb. 6.56, und mit allen drei Schrauben (links) in R作者: 沉默 時(shí)間: 2025-3-24 15:43 作者: FOLLY 時(shí)間: 2025-3-24 19:12 作者: frugal 時(shí)間: 2025-3-25 03:14
Young Hyun Park,Young Min Kim,Jae Yong Han differenzierte Antworten. Geht es an dieser Nahtstelle zwischen Tragwerk und Fundament doch besonders um den Ma?- oder Toleranzausgleich zwischen den in dieser Beziehung recht verschiedenen Gewerken Stahlbau und Massivbau. Letztlich also um nichts geringeres als um die Vereinfachung und Beschleunig作者: Irrigate 時(shí)間: 2025-3-25 05:09
1064-3745 ebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition. serves as an invaluable aid to scientists, educators, and the advanced general audience a978-1-4939-9009-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 慢慢啃 時(shí)間: 2025-3-25 10:50
Culture and Host Transfer of , Oocytes for Maternal mRNA Depletion and Genome Editing Experiments,obtaining, culturing, transferring, and fertilizing . oocytes are described. This method has typically been used to alter maternal gene function by antisense oligonucleotide-mediated mRNA knockdown, but is also useful for mRNA or protein overexpression, including the expression of genome-editing rea作者: 冬眠 時(shí)間: 2025-3-25 13:54 作者: Control-Group 時(shí)間: 2025-3-25 19:31
Book 2019Latest editionoducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and fully updated, .Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition. serves as an invaluable aid to scientists, educators, and the advanced general audience a作者: 血統(tǒng) 時(shí)間: 2025-3-25 23:37
Culture and Host Transfer of , Oocytes for Maternal mRNA Depletion and Genome Editing Experiments,immediately after fertilization, it is often desirable to perturb gene function before this occurs. Oocytes can be experimentally manipulated in vitro, prior to maturation, and subsequently fertilized or otherwise activated to develop, and then observed for any embryological defects. Available metho作者: 從屬 時(shí)間: 2025-3-26 04:08 作者: Neutral-Spine 時(shí)間: 2025-3-26 07:55
Assaying NanoLuc Luciferase Activity from mRNA-Injected , Embryos, analysis of post-transcriptional regulatory events has relied upon the use of chimeric reporter mRNAs that encode firefly luciferase fused to potential regulatory sequences. A new and more sensitive luciferase developed recently called NanoLuc has the potential to improve reporter studies and provi作者: 斥責(zé) 時(shí)間: 2025-3-26 11:14 作者: glowing 時(shí)間: 2025-3-26 13:21
Membrane Microdomains as Platform to Study Membrane-Associated Events During Oogenesis, Meiotic Matamete interaction and possibly fusion in African clawed frog .. Here we describe our protocol for identifying and analyzing molecular and cellular machinery that contributes to a variety of biological processes in the course of oogenesis, oocyte maturation, egg fertilization, and early embryogenesis作者: archaeology 時(shí)間: 2025-3-26 18:22 作者: progestin 時(shí)間: 2025-3-26 21:55
Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos,ocols for analysis of protein-DNA and protein-protein interactions in tissues and cells isolated from the developing chick. These assays are aimed at the identification of interactions between transcription factors and regulatory elements in the genome, and, in combination with functional assays, ca作者: Concerto 時(shí)間: 2025-3-27 03:16 作者: 亞麻制品 時(shí)間: 2025-3-27 07:12
RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq),are therefore crucial for identifying novel regulators and the?understanding of their function. However, in situ methods do not resolve global gene expression, and sequencing-based methods usually do not provide spatial information. Here, we describe tomo-seq, a method that combines classical histol作者: temperate 時(shí)間: 2025-3-27 13:12
Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos,to reversibly bind together an “anchor” protein and a “bait” protein. Proteins can therefore be directed to specific subcellular compartments, altering biological processes such as cell polarity and signaling. I detail methods for achieving transient expression of fusion proteins encoding the phytoc作者: DEMUR 時(shí)間: 2025-3-27 15:21
Ex Utero Culture and Imaging of Mouse Embryos,vailability and improvement of a wide variety of genetically encoded fluorescent proteins have provided indispensable tools to visualize cells and subcellular features in living organisms. It is now possible to generate genetically modified mouse lines expressing several spectrally distinct fluoresc作者: HEAVY 時(shí)間: 2025-3-27 18:55 作者: 犬儒主義者 時(shí)間: 2025-3-27 22:46 作者: 兇猛 時(shí)間: 2025-3-28 04:40
Manipulation of Developmental Function in Turtles with Notes on Alligators,n comparative and evolutionary developmental biology makes protocols for the study of reptile embryos invaluable resources. The relatively large size, seasonal breeding, and long gestation times of turtles epitomize the challenges faced by the developmental biologist. We describe protocols for the p作者: painkillers 時(shí)間: 2025-3-28 07:53 作者: 鴕鳥(niǎo) 時(shí)間: 2025-3-28 14:07
Visualizing the Balbiani Body in Zebrafish Oocytes,s isolation, histology, staining, and microscopic examination of early zebrafish oocytes. These techniques can be applied to visualize gene products that are localized to the Balbiani body, and when applied to mutants can be used to decipher molecular and genetic pathways acting in Balbiani body dev作者: 粗俗人 時(shí)間: 2025-3-28 15:24 作者: 事情 時(shí)間: 2025-3-28 19:05 作者: 怎樣才咆哮 時(shí)間: 2025-3-29 00:09 作者: 施加 時(shí)間: 2025-3-29 04:32 作者: avenge 時(shí)間: 2025-3-29 10:37 作者: CHART 時(shí)間: 2025-3-29 15:07 作者: 性滿(mǎn)足 時(shí)間: 2025-3-29 15:58 作者: 蕁麻 時(shí)間: 2025-3-29 20:48
Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos,the identification of interactions between transcription factors and regulatory elements in the genome, and, in combination with functional assays, can be used for the delineation of gene regulatory circuits.作者: Toxoid-Vaccines 時(shí)間: 2025-3-30 00:45 作者: Jargon 時(shí)間: 2025-3-30 05:37
Germ Cell Transplantation in Avian Species,s, this has mostly relied on primordial germ?cell (PGC) transplantation for efficient germline transmission. In this chapter, we describe how to isolate PGCs from avian embryos and produce germline chimeras through transplantation of donor PGCs to recipient embryos.作者: 疲憊的老馬 時(shí)間: 2025-3-30 11:43
1064-3745 advice from the expertsThis detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are includ作者: MAG 時(shí)間: 2025-3-30 14:18
Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos,ers, chromosomes, and nuclei in whole-mount embryos, even when they are hundreds of micrometers removed from the embryo’s surface. Though the described methods were optimized for microtubule assemblies, they have also proven useful for the visualization of other proteins.作者: Ambiguous 時(shí)間: 2025-3-30 18:17 作者: 搬運(yùn)工 時(shí)間: 2025-3-30 23:58
Manipulation of Developmental Function in Turtles with Notes on Alligators,en adapted and optimized from methods used for frog, chick, and mouse embryos, it is likely that they could be used for other reptilian species. Notes are included for alligator embryos where appropriate.作者: crescendo 時(shí)間: 2025-3-31 03:29
Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis,ugh a syringe, and then used a fine injection needle to wick up Balbiani bodies by capillary action with minimal buffer solution. Using this protocol we collected sufficient material for proteomic analysis of the zebrafish Balbiani body.作者: 薄膜 時(shí)間: 2025-3-31 07:39 作者: armistice 時(shí)間: 2025-3-31 12:19
Experimental Manipulation of Ploidy in Zebrafish Embryos and Its Application in Genetic Screens,haploid or tetraploid states. We also describe methodologies to verify the ploidy of embryos and the utility of ploidy manipulation in expediting the isolation of mutations using both forward and reverse genetic strategies in zebrafish.作者: GNAT 時(shí)間: 2025-3-31 15:52 作者: 下船 時(shí)間: 2025-3-31 17:44
Thao Nguyen,Timothy J. Mitchison,Martin Wührhsvollen Randbedingungen auf Baustellen und die im Bauwesen typische Unikatbauweise unterliegen Beton-3D-Druckverfahren besonderen Herausforderungen. Die innovativen Technologien stehen darüber hinaus im wirtschaftlichen Wettbewerb zu konventionellen Bauverfahren. Um die Wirtschaftlichkeit und Proze作者: Brocas-Area 時(shí)間: 2025-4-1 01:38 作者: ALIAS 時(shí)間: 2025-4-1 04:45
