標題: Titlebook: Ubiquitin-Proteasome Protocols; Cam Patterson,Douglas M. Cyr Book 2005 Humana Press 2005 Endoplasmatisches Reticulum.Termination.biochemis [打印本頁] 作者: Bunion 時間: 2025-3-21 19:27
書目名稱Ubiquitin-Proteasome Protocols影響因子(影響力)
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書目名稱Ubiquitin-Proteasome Protocols網(wǎng)絡公開度學科排名
書目名稱Ubiquitin-Proteasome Protocols被引頻次
書目名稱Ubiquitin-Proteasome Protocols被引頻次學科排名
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書目名稱Ubiquitin-Proteasome Protocols年度引用學科排名
書目名稱Ubiquitin-Proteasome Protocols讀者反饋
書目名稱Ubiquitin-Proteasome Protocols讀者反饋學科排名
作者: DIS 時間: 2025-3-21 20:32 作者: 迷住 時間: 2025-3-22 01:28
Chang-Wei Liu,Elizabeth Strickland,George N. DeMartino,Philip J. ThomasMost authors who have investigated the mechanism of the production of neurological deficit by disease of the cervical spine have analysed various compressive factors arising from spinal structures, which may be treated by removal of these compressive factors by surgery.作者: avulsion 時間: 2025-3-22 07:19
Edward G. Mimnaugh,Leonard M. NeckersNosocomial infections are the most frequent complications observed in surgical patients. In recent years reductions of post-operative infections have been mostly due to a correct use of prophylactic measures such as pre-operative selective bowel decontamination, adequate antibiotic prophylaxis, and better anaesthetic and intensive care management.作者: Rebate 時間: 2025-3-22 11:21
Methods in Molecular Biologyhttp://image.papertrans.cn/u/image/940216.jpg作者: 打算 時間: 2025-3-22 14:52 作者: WAIL 時間: 2025-3-22 19:22
Cam Patterson,Douglas M. CyrIncludes supplementary material: 作者: TOXIN 時間: 2025-3-22 23:59 作者: 噱頭 時間: 2025-3-23 05:07 作者: hedonic 時間: 2025-3-23 05:55
Purification of Proteasomes, Proteasome Subcomplexes, and Proteasome-Associated Proteins From Buddind, and the base. A variety of activity assays and native gel procedures are available to evaluate purified proteasomes functionally. When coupled with the genetic methods available in yeast, these biochemical procedures allow for detailed functional analysis of this unique protein complex.作者: 桶去微染 時間: 2025-3-23 12:25 作者: Eeg332 時間: 2025-3-23 16:26 作者: LEER 時間: 2025-3-23 19:10 作者: OUTRE 時間: 2025-3-23 22:47
Purification of E1 and E1-Like Enzymesman red blood cells in yields (2–4 nmol/U of blood) that make this an attractive alternative to expression of the proteolytically labile recombinant protein. In addition, two stoichiometric activity assays are described that rely on formation of the E1 .I-ubiquitin thiol ester and ubiquitin [2,8-.H]adenylate intermediates.作者: 等級的上升 時間: 2025-3-24 04:13 作者: 使增至最大 時間: 2025-3-24 06:37 作者: 凝乳 時間: 2025-3-24 13:31 作者: 合同 時間: 2025-3-24 15:38
Shahri Raasi,Cecile M. Pickarto effective prevention. This has always been one of our worst kept secrets. Equally conflicts of interest [.] assume particular relevance in an occupation whose foundation values demand a robust ethical identity. This is the topic of this essay.作者: Forage飼料 時間: 2025-3-24 20:24 作者: Fluctuate 時間: 2025-3-24 23:24
Small-Molecule Inhibitors of Proteasome Activityl-molecule compounds blocking its activities. Besides being candidates for drugs against cancer, autoimmune diseases, inflammation, or stroke, specific proteasome inhibitors are indispensable tools for biochemical and cell biology investigations of the proteasome and proteasome-ubiquitin system. Num作者: 原來 時間: 2025-3-25 07:06
Purification of E1 and E1-Like Enzymeske proteins required for their subsequent conjugation to cellular targets. The general physical and mechanistic features of the E1 family appear well conserved. Formation of an obligatory E1-ubiquitin thiol ester intermediate forms the basis of a one-step covalent purification of the enzyme on ubiqu作者: outer-ear 時間: 2025-3-25 09:25
Assays for RING Family Ubiquitin Ligasescontrol, tumor suppression, and many signaling pathways. Ubiquitination of proteins occurs through an enzymatic cascade of three discrete steps, which results in covalent attachment of ubiquitin to the substrate. The first two steps in this cascade involve the activating and conjugating enzymes, E1 作者: Coronary-Spasm 時間: 2025-3-25 15:37 作者: 起來了 時間: 2025-3-25 18:08 作者: 磨碎 時間: 2025-3-25 22:10
Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proluding ATPase activity, polyubiquitin-chain binding activity, deubiquitination activity, chaperone-like activity, and substrate remodeling activity. The concerted action of these activities leads to efficient degradation of protein substrates by the 26S proteasome. In this chapter we describe protoc作者: 美色花錢 時間: 2025-3-26 03:41 作者: inspiration 時間: 2025-3-26 05:25 作者: neolith 時間: 2025-3-26 10:25 作者: Paraplegia 時間: 2025-3-26 16:28 作者: 聯(lián)邦 時間: 2025-3-26 19:22 作者: 廚房里面 時間: 2025-3-26 23:43
Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradationtant and misfolded membrane proteins. When supplemented with canine pancreas microsomal membranes, the RRL system faithfully carries out ER targeting, translocation, glycosylation, and membrane integration events and therefore provides a ready source of 35S-labeled protein with defined transmembrane作者: LARK 時間: 2025-3-27 01:55
Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterizationcharacterized. This chapter describes biochemical methods that can be used to characterize the function and substrate specificity of DUBs. Methods described will include: fluorescence assay using ubiquitin-amidomethylcoumarin (AMC); a high-performance liquid chromatography assay using ubiquitin ethy作者: 誓言 時間: 2025-3-27 09:21 作者: 你不公正 時間: 2025-3-27 13:02 作者: collagen 時間: 2025-3-27 15:00
N-Terminal Ubiquitinations—an E3, that is followed by generation of the polyubiquitin degradation signal. For most substrates, it is believed, though it has been demonstrated experimentally only for a few, that the first ubiquitin moiety is conjugated, via its C-terminal Gly. residue, to an ε-NH. group of an internal Lys re作者: 材料等 時間: 2025-3-27 21:42 作者: SLAG 時間: 2025-3-28 01:54
Book 2005 basic biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochemical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-proteasome-dependent functions. State-of-the-art and user-friendly, Ubiquitin-Proteasome Pro作者: adjacent 時間: 2025-3-28 05:33
disease, as well as the experience of the surgeon, should also be taken in consideration. It is clear that the simpler and the less traumatic the procedure, the better it is tolerated by the patient, who, particularly in cases of infectious or neoplastic processes, is often quite sick.作者: 性冷淡 時間: 2025-3-28 08:11
Manabu Furukawa,Paul S. Andrews,Yue Xiongctomy to a higher level. On removal of another lamina, a clearly circumscribed tumour was found and removed. For the first time in the history of medicine a patient operated on for a spinal cord tumour recovered from the operation completely and gradually resumed his previous occupation.作者: insecticide 時間: 2025-3-28 12:43 作者: 凹處 時間: 2025-3-28 16:03 作者: 悲觀 時間: 2025-3-28 21:15
Eric Carlson,Nathan Bays,Larry David,William R. Skachreal-life problems in all areas including business and finance, science, engineering, industry, cyberspace, bioinformatics, automation, robotics, medicine and biomedicine, and human-machine interactions..作者: 財主 時間: 2025-3-29 00:15
Shu-Bing Qian,Jack R. Bennink,Jonathan W. Yewdell images is directly related to the mechanical properties of the sample and the coupling fluid, which has to be present in SAM to allow the propagation of acoustic waves between the focusing transducer device (“acoustic lens”) and the sample.作者: 潔凈 時間: 2025-3-29 03:53 作者: famine 時間: 2025-3-29 11:17 作者: Prologue 時間: 2025-3-29 12:12 作者: scrutiny 時間: 2025-3-29 15:38
Quantitating Defective Ribosome Productsy degraded nascent proteins. Quantitating DRiPs entails employing a standard pulse-chase protocol using radiolabeled amino acids. Protein degradation kinetics can be determined by either acid precipitation or SDS-PAGE. The introduction of proteasome inhibitors enables quantitation of proteasome-mediated protein degradation in vivo.作者: Incorporate 時間: 2025-3-29 19:49 作者: Interstellar 時間: 2025-3-30 02:02 作者: 流動才波動 時間: 2025-3-30 04:37
Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradationlase to proteasomal degradation. In this chapter we describe ornithine decarboxylase/antizyme-based in vivo and in vitro systems for the measurement of ATP-dependent, ubiquitin-independent proteasomal degradation, as well as the application of ornithine decarboxylase as a reporter for the targeting of proteins to the 26S proteasome.作者: 尖牙 時間: 2025-3-30 08:28
Small-Molecule Inhibitors of Proteasome Activityesting the mechanism of proteolysis catalyzed by the enzyme. In this chapter we discuss the most common applications of available proteasome inhibitors. In addition to the best-known competitive inhibitors, we also describe the benefits from the use of allosteric inhibitors, which induce distinct bu作者: CHASE 時間: 2025-3-30 14:11
Assays for RING Family Ubiquitin Ligases protein subunits, or proteins with intrinsic RING domains, to catalyze the formation of polyubiquitin chains. In this chapter we describe step by step protocols to assay for the activity of the RING finger-type of E3 ligase both in vitro and in vivo.作者: 針葉樹 時間: 2025-3-30 17:23
Reconstitution of Endoplasmic Reticulum-Associated Degradation Using Yeast Membranes and Cytosolhas been elucidated in part through an in vitro ERAD assay using reagents prepared from a model eukaryote, the yeast .. This chapter describes this in vitro ERAD assay in detail, and special considerations when performing the assay are noted.作者: OTTER 時間: 2025-3-30 20:47
Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradationh membrane-associated and cytosolic components are amenable to biochemical and pharmacological manipulation. Here we describe techniques for preparing translation- and degradation-competent RRL, affinity depletion, identification of cytosolic factors involved in degrading the cystic fibrosis transme作者: 波動 時間: 2025-3-31 03:21
Assays for Proteasome Assembly and Maturationme present in cells at steady state are analyzed by gel filtration and immunoblotting after sodium dodecyl sulfate (SDS)- and native polyacrylamide gel electrophoresis (PAGE). The kinetics of proteasome assembly is followed by pulse chase detection of β subunit maturation or of Ump1 degradation.作者: 夸張 時間: 2025-3-31 05:10
Maria Gaczynska,Pawel A. Osmulski alternatives available at the time was better than nothing for this unfortunate group. It allowed 20% to return to life in the community. However, as the authors emphasize this became history with the discovery in the 1950’s of the major tranquilizers and effective pharmacological agents for the tr作者: 窗簾等 時間: 2025-3-31 10:43
Chaim Kahana,Yuval Reissn made. The contributions are written by specialists in the given fields and constitute the first part of each volume. In the second part of each volume, we publish detailed descriptions of standard operative procedures, furnished by experienced clinicians; in these articles the authors describe the作者: neologism 時間: 2025-3-31 15:27
Carol E. Parker,Maria R. Warren,David R. Loiselle,Nedyalka N. Dicheva,Cameron O. Scarlett,Christoph avior using a real-world data-set. Next, reinforcement learning is applied on top of the pre-trained agents in a simulated environment, to allow them to adapt to changes in the platform. Our framework provides an ideal playground for ride-hailing platforms to experiment with platform-specific parame作者: 整理 時間: 2025-3-31 20:08 作者: Adulate 時間: 2025-4-1 00:29
Nathaniel S. Russell,Keith D. Wilkinsonn 13 classification datasets and compared to four mainstream evolutionary algorithms. The experimental results showed that CEA was able to achieve better classification performance using similar or fewer features than the other algorithms.作者: Reverie 時間: 2025-4-1 04:15 作者: Infelicity 時間: 2025-4-1 06:47
R. Jürgen Dohmen,Markus K. London,Christoph Glanemann,Paula C. Ramosnitial operation, by an interview and anorectal manometry...: The mean number of defecations/24h were significantly higher in LAR patients 4.1 ± 0.7 versus 2.0 ± 1.5 in CAA patients, 2.2 ± 1 in patients with PCAA and 2.3 ± 1.8 in PLAR patients (p<0.05 when LAR group is compared to the other groups).作者: 忘恩負義的人 時間: 2025-4-1 12:42 作者: 按時間順序 時間: 2025-4-1 14:49
y of the lesion, its extension, its relationship with the neural structures, its probable nature, its texture and vascular supply, the degree of resection one wishes to accomplish, and the need to stabilize the spine. Furthermore, the age and clinical status of the patient, and the prognosis of the
