標(biāo)題: Titlebook: Synthetic mRNA; Production, Introduc Robert E. Rhoads Book 2016 Springer Science+Business Media, LLC, part of Springer Nature 2016 gene the [打印本頁] 作者: Coagulant 時間: 2025-3-21 17:33
書目名稱Synthetic mRNA影響因子(影響力)
書目名稱Synthetic mRNA影響因子(影響力)學(xué)科排名
書目名稱Synthetic mRNA網(wǎng)絡(luò)公開度
書目名稱Synthetic mRNA網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Synthetic mRNA被引頻次
書目名稱Synthetic mRNA被引頻次學(xué)科排名
書目名稱Synthetic mRNA年度引用
書目名稱Synthetic mRNA年度引用學(xué)科排名
書目名稱Synthetic mRNA讀者反饋
書目名稱Synthetic mRNA讀者反饋學(xué)科排名
作者: GRE 時間: 2025-3-21 22:27
f 2008. These crises have been metaphorically interpreted as “black swans” by Nassim Taleb in a book that made a worldwide impression, . (.). A “black swan” is an event that is very unlikely and not normally to be expected (i.e., an “outlier”) but which, if it does occur, will have extreme and possi作者: 怕失去錢 時間: 2025-3-22 00:28 作者: overhaul 時間: 2025-3-22 07:08
ncluding the reversal of the oil companies’ attitude in the early 1990s and the current fossil fuel industry’s standpoint. It goes on to show that the global transition to carbon neutrality is possible, desirable and cost-effective in the long term but that the mobilization of society, companies and作者: Cardiac-Output 時間: 2025-3-22 10:11
Joanna Kowalska,Franck Martin,Jacek Jemielityncluding the reversal of the oil companies’ attitude in the early 1990s and the current fossil fuel industry’s standpoint. It goes on to show that the global transition to carbon neutrality is possible, desirable and cost-effective in the long term but that the mobilization of society, companies and作者: 協(xié)議 時間: 2025-3-22 13:06
Josephin M. Holstein,Daniela Stummer,Andrea Rentmeistererge of what many are calling the "second information revolution," based on ubiquitous access to both computing and information. Handheld communication devices will become portable and even wearable remote control devices for both the social and physical worlds. At the same time, access to informati作者: fastness 時間: 2025-3-22 17:19 作者: Genistein 時間: 2025-3-22 23:27 作者: 山羊 時間: 2025-3-23 05:02 作者: 太空 時間: 2025-3-23 05:57
Daphné Benteyn Ph.D.,Carlo Heirman,Kris Thielemans,Aude Bonehillerge of what many are calling the "second information revolution," based on ubiquitous access to both computing and information. Handheld communication devices will become portable and even wearable remote control devices for both the social and physical worlds. At the same time, access to informati作者: ALLAY 時間: 2025-3-23 11:51
erge of what many are calling the "second information revolution," based on ubiquitous access to both computing and information. Handheld communication devices will become portable and even wearable remote control devices for both the social and physical worlds. At the same time, access to informati作者: 使堅(jiān)硬 時間: 2025-3-23 15:50 作者: 滴注 時間: 2025-3-23 18:08 作者: antidote 時間: 2025-3-24 01:39
David Selmeczi,Thomas Steen Hansen,?zcan Met,Inge Marie Svane,Niels B. Larsenerge of what many are calling the "second information revolution," based on ubiquitous access to both computing and information. Handheld communication devices will become portable and even wearable remote control devices for both the social and physical worlds. At the same time, access to informati作者: Postmenopause 時間: 2025-3-24 05:23
Sebastian Kreiter,Mustafa Diken,Abderraouf Selmi,Jutta Petschenka,?zlem Türeci,Ugur Sahinerge of what many are calling the "second information revolution," based on ubiquitous access to both computing and information. Handheld communication devices will become portable and even wearable remote control devices for both the social and physical worlds. At the same time, access to informati作者: 玉米 時間: 2025-3-24 07:12 作者: 小教堂 時間: 2025-3-24 14:07
Solenne Bire,Nicole Ishac,Florence Rouleux-Bonninerge of what many are calling the "second information revolution," based on ubiquitous access to both computing and information. Handheld communication devices will become portable and even wearable remote control devices for both the social and physical worlds. At the same time, access to informati作者: 通知 時間: 2025-3-24 16:37 作者: Bmd955 時間: 2025-3-24 23:02
erge of what many are calling the "second information revolution," based on ubiquitous access to both computing and information. Handheld communication devices will become portable and even wearable remote control devices for both the social and physical worlds. At the same time, access to informati作者: 種植,培養(yǎng) 時間: 2025-3-25 00:29
Gayathri R. Devi Ph.D.,Sritama Natherge of what many are calling the "second information revolution," based on ubiquitous access to both computing and information. Handheld communication devices will become portable and even wearable remote control devices for both the social and physical worlds. At the same time, access to informati作者: 骨 時間: 2025-3-25 06:31
Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequenceslity to activate the innate immune response against exogenous (virus-like) RNA, and can be efficiently translated into protein. Synthetic methods have also been developed to produce mRNA with unique investigational properties such as photo-cross-linking, fluorescence emission, and attachment of liga作者: 使乳化 時間: 2025-3-25 09:05
Synthetic Capped mRNAs for Cap-Specific Photo-Cross-Linking Experimentscation and studies on cap-binding partners can be significantly advanced using tailored chemical tools such as synthetic cap analogues or RNAs carrying modified cap structures. Here we provide protocols for the production of mRNAs specifically labeled within the 5′ cap with a nucleoside capable of b作者: inclusive 時間: 2025-3-25 15:34
Enzymatic Modification of 5′-Capped RNA and Subsequent Labeling by Click Chemistryes of biomolecules in vitro and even in cellular environments. Herein, we describe a chemoenzymatic method to site specifically label 5′-capped model mRNAs independent of their sequence. A trimethylguanosine synthase was engineered to introduce alkyne, azido, or 4-vinylbenzyl moieties to the 5′-cap.作者: Substitution 時間: 2025-3-25 17:03
Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppGrthermore, fluorescent tags prove invaluable for tracking RNA molecules in cells. Here, we describe an efficient synthesis of a fluorescent cap analog, anthranioyl-GTP, its purification, and in vitro cap labeling of transcribed mRNA catalyzed by the recombinant vaccinia capping enzyme to produce ant作者: MONY 時間: 2025-3-25 23:12 作者: Madrigal 時間: 2025-3-26 03:09 作者: 使成波狀 時間: 2025-3-26 05:26
Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cellscer patients. Among several available techniques for DC modification, mRNA electroporation is an interesting technique due to the favorable characteristics of mRNA. Antigen expression level and duration can be increased by multiple optimizations of an antigen-encoding mRNA template. Here, we describ作者: nonsensical 時間: 2025-3-26 10:27 作者: 不可思議 時間: 2025-3-26 12:54 作者: SKIFF 時間: 2025-3-26 20:00
Large-Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systemsncer immunotherapy. Therapeutic application requires methods to efficiently electroporate and transfect millions of immune cells in a fast process with high cell survival. Continuous flow of suspended dendritic cells through a channel incorporating spatially separated microporous meshes with a synch作者: 代替 時間: 2025-3-26 23:30 作者: 發(fā)生 時間: 2025-3-27 03:53
Transfecting Human Monocytes with RNAarea of intense investigation in both basic and clinical research. Herein we describe an efficient method to deliver mRNA (messenger RNA) or siRNA (small interfering RNA) into human monocytes by electroporation. This method can be applied in the laboratory to monocytes isolated via magnetic bead-bas作者: BRIBE 時間: 2025-3-27 07:07 作者: 燕麥 時間: 2025-3-27 11:18
Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair ortance. One such protein is photolyase which rapidly removes UV-induced DNA damages, but this enzyme is absent in humans. Here, we apply a novel mRNA-based platform to achieve functional nonhuman photolyase production in cultured human keratinocytes. Transfection of nucleoside-modified mRNA encodin作者: 被告 時間: 2025-3-27 16:16
Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Canceined peptides of tumor-associated antigens, (b) total protein isolated from the tumor cell, (c) autologous total RNA isolated from the tumor cell, (d) synthetic tumor-antigen-encoding mRNA, or (e) genes that encode for specific tumor-associated antigens. Introduction of tumor-associated antigen(s) a作者: 厚顏 時間: 2025-3-27 20:07
Synthetic Capped mRNAs for Cap-Specific Photo-Cross-Linking Experimentseing photo-activated, either 6-thioguanosine or 7-methyl-6-thioguanosine, which can be used in photo-cross-linking experiments to identify or characterize cap-binding biomolecules. We also describe a protocol for the cross-linking experiments with capped RNAs to map histone H4 cap-binding pocket.作者: STENT 時間: 2025-3-27 22:34
Large-Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systemsronized electrical pulsing sequence can yield dendritic cell transfection rates of >75?% with survival rates of >90?%. This chapter describes the instrumentation and methods needed for the efficient transfection by electroporation of millions of dendritic cells in one continuous flow process.作者: CRATE 時間: 2025-3-28 02:52 作者: Demonstrate 時間: 2025-3-28 10:06 作者: 手榴彈 時間: 2025-3-28 11:40 作者: 國家明智 時間: 2025-3-28 15:36
Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cellsstics of mRNA. Antigen expression level and duration can be increased by multiple optimizations of an antigen-encoding mRNA template. Here, we describe different molecular modifications to a WT1-encoding mRNA construct in order to increase antigen expression and the subsequent introduction of mRNA into DCs.作者: sclera 時間: 2025-3-28 20:25
GMP-Grade mRNA Electroporation of Dendritic Cells for Clinical Useand viability. In order to comply with the directives and guidelines defined by the regulatory authorities, we report here a standardized and reproducible method for the manufacturing of clinical-grade mRNA-transfected DC.作者: 破譯密碼 時間: 2025-3-29 01:24 作者: 獎牌 時間: 2025-3-29 06:20
Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequencesse in regenerative medicine therapies, and engineering the genome by making specific alterations in DNA. This introductory chapter provides background information relevant to the following 20 chapters of this volume that present protocols for these applications of synthetic mRNA.作者: Detonate 時間: 2025-3-29 09:37 作者: Infant 時間: 2025-3-29 13:59
Transfecting Human Monocytes with RNAon System. We further demonstrate that electroporation of monocytes with RNA represents a robust and highly relevant approach to modify monocytes for cell-based therapies. Last, the procedure described can readily be adapted to monocytes from different species, hence facilitating research in animal models.作者: ILEUM 時間: 2025-3-29 19:01
Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cance preparation. Here, we described a method of: (a) preparing and delivering synthetic FOXP3 mRNA into human DCs, (b) generating mature DCs, (c) generating FOXP3-specific cytotoxic T lymphocytes, and (d) evaluating cytotoxicity of FOXP3-specific cytotoxic T lymphocytes against inflammatory breast cancer cells.作者: Etching 時間: 2025-3-29 23:09
wo independent variables—culture and cultural heterogeneity—correctly identified more than eight out of ten states in terms of whether they did not or did ratify the ERA. The empirical indicator of culture was the state rate of labor force participation among married, husband-present women who had a作者: 才能 時間: 2025-3-30 03:39
t merely a descriptive language for financial risks (representational view of language) but a language that produces a “speech act” (pragmatic view of language) in the sense of financial models understood as speech acts: the financial . speaks and transforms the world through financial instruments a作者: FIG 時間: 2025-3-30 06:04
Robert E. Rhoadsgs, memories, traditions and identity contents. This symbolic conflict is, for us, an illustrative example of the importance of the role of names and their historicity in social psychology, in order to better understand the identity and memory processes they underlie and thus contribute to a more sy作者: Agnosia 時間: 2025-3-30 11:37
nse shows that it further increases the level of uncertainty about the future. In conclusion, the most likely projection is that mankind will be confronted in the current and following centuries with the dual threat of climate change and severe geoengineering impacts.作者: Charitable 時間: 2025-3-30 15:52 作者: 供過于求 時間: 2025-3-30 18:52
Josephin M. Holstein,Daniela Stummer,Andrea Rentmeistereading international experts who discuss issues surrounding the impact of instant access on cities, daily lives, transportation, privacy, social and economic networks, community and education. .978-90-481-7365-5978-1-4020-5427-3Series ISSN 0924-5499 Series E-ISSN 2215-0072 作者: parallelism 時間: 2025-3-31 00:20
