作者: 外來 時(shí)間: 2025-3-21 20:22
Madalena Chaves,Hidde de Jongetti-Fronteczak , & Johnson, 2001). Many teachers come to a workshop or course on guidance (which they may term “discipline” when describing it to others) expecting to learn a magic formula that will instantly transform their class of very active, noisy, inattentive little ones into a picture perfec作者: Tinea-Capitis 時(shí)間: 2025-3-22 03:16 作者: 圓桶 時(shí)間: 2025-3-22 05:15 作者: inferno 時(shí)間: 2025-3-22 10:48 作者: colony 時(shí)間: 2025-3-22 14:15 作者: innate 時(shí)間: 2025-3-22 17:24
Valentin Zulkowerems of northern North America and Russia. For the most part, the authors discuss relatively long-term patterns of vegetation and soil recovery and rehabilitation. It is refreshing to review such a collection of chapters because the standard for many years has been to limit applied investigations tre作者: 虛度 時(shí)間: 2025-3-22 23:14
Valentin Zulkowerpment, and wildlife. Our approach is to emphasize the ecosystem interactions that tie people to wildlife, and wildlife to the land, thus viewing industrial development in terms of its influence in altering ecosystem relationships. We acknowledge that ecosystems are dynamic, and that changes taking p作者: Ingredient 時(shí)間: 2025-3-23 05:20
Deepti Vipin,Zoya Ignatova,Thomas E. Gorochowskir ability to communicate to policymakers clear messages regarding complex biological and social interactions will become essential to sustainable development. Major environmental threats include, but are not limited to, fragmentation and habitat loss, over-exploitation, climate change, pollution and作者: gospel 時(shí)間: 2025-3-23 07:25 作者: AXIS 時(shí)間: 2025-3-23 11:39
Mahmoud Khazim,Elisa Pedone,Lorena Postiglione,Diego di Bernardo,Lucia Marucciirection of influence is difficult to interpret and causal conclusions cannot be drawn. The few existing longitudinal studies on this topic have mainly focused on the normal direction of the causal relationship, that is, on the impact of working time arrangements on work-family conflict over time. T作者: 獨(dú)白 時(shí)間: 2025-3-23 16:31
Eva Balsa-Canto,Lucia Bandiera,Filippo Menolascinaalth and well-being. Working time organization is becoming a key factor on account of new technologies, market globalization, economic competition, and extension of social services to general population, involving more and more people in continuous assistance and control of the work processes over t作者: Carbon-Monoxide 時(shí)間: 2025-3-23 22:02
Lucia Bandiera,David Gomez-Cabeza,Eva Balsa-Canto,Filippo Menolascinas shift work an environmental demand that makes “balancing” work and family more difficult, or is it a viable tool for alleviating inherent conflicts between work and family life? A casual perusal of the scholarly and popular literature will result in evidence supporting both positions, suggesting t作者: municipality 時(shí)間: 2025-3-24 02:15
Evangelos-Marios Nikolados,Andrea Y. Wei?e,Diego A. Oyarzún role of individual differences in circadian rhythm characteristics on this relationship. The aim of the study was to examine whether morningness and circadian type traits (flexibility of sleeping habits and inability to overcome drowsiness), which have been found to be related to tolerance to shift作者: acclimate 時(shí)間: 2025-3-24 02:38 作者: 圓柱 時(shí)間: 2025-3-24 09:27 作者: Obsequious 時(shí)間: 2025-3-24 12:21
Giuliano Bonfá,Federica Cella,Velia Sicilianont of many forms of illness in early life, the disabilities that bear some relationship to ageing have been thrown into increasing prominence. It is logical to give consideration to mental disorder in this context in that, if first admission rates to mental hospitals are taken as an index, the expec作者: 革新 時(shí)間: 2025-3-24 16:09
nt of many forms of illness in early life, the disabilities that bear some relationship to ageing have been thrown into increasing prominence. It is logical to give consideration to mental disorder in this context in that, if first admission rates to mental hospitals are taken as an index, the expec作者: 無情 時(shí)間: 2025-3-24 19:10 作者: NAVEN 時(shí)間: 2025-3-25 01:35 作者: 種屬關(guān)系 時(shí)間: 2025-3-25 03:24
,Prediction of Cellular Burden with Host–Circuit Models,sign parameters on both burden and circuit functionality. Our approach relies on a new generation of computational models for microbial growth that can flexibly accommodate resource bottlenecks encountered in gene circuit design. Adoption of this modeling paradigm can facilitate fast and robust design cycles in synthetic biology.作者: Sad570 時(shí)間: 2025-3-25 09:37
Book 2021l techniques to model and simulate biological systems, redesign biological systems, setting up of an automated biolaboratory, step-by-step guide on how to perform computer aided design, RNA sequencing, microfluidics -using bacterial cell free extracts, live mammalian cells, computational and experim作者: Migratory 時(shí)間: 2025-3-25 13:21
Qualitative Modeling, Analysis and Control of Synthetic Regulatory Circuits,s. We also briefly present the problem of controlling synthetic circuits, an emerging topic that could profit from the capacity of qualitative modeling approaches to rapidly scan a space of design alternatives.作者: HUSH 時(shí)間: 2025-3-25 19:41 作者: 換話題 時(shí)間: 2025-3-25 21:20 作者: 國家明智 時(shí)間: 2025-3-26 01:46 作者: CODA 時(shí)間: 2025-3-26 05:27
Optimal Experimental Design for Systems and Synthetic Biology Using AMIGO2,OED can be used to define the set of experiments that would (a) identify the best model or (b) improve the identifiability of unknown parameters. In this chapter, we present a detailed practical procedure to compute optimal experiments using the AMIGO2 toolbox.作者: 被告 時(shí)間: 2025-3-26 11:26 作者: Lament 時(shí)間: 2025-3-26 14:51
Stochastic Differential Equations for Practical Simulation of Gene Circuits,ules of the species involved is not extremely low. Here, we describe methods and a computational framework to simulate a population of cells containing gene circuits of interest. These methods account for both intrinsic and extrinsic noise sources, and allow us to have both individual cell-related s作者: 航海太平洋 時(shí)間: 2025-3-26 17:00
Using Models to (Re-)Design Synthetic Circuits, into novel gene networks. Here, we present a guide for biologists to build and utilize models of gene networks (synthetic or natural) to analyze dynamical properties of these networks while considering the low numbers of molecules inside cells that results in stochastic gene expression. We start by作者: 沖擊力 時(shí)間: 2025-3-26 21:40 作者: Expostulate 時(shí)間: 2025-3-27 04:44 作者: 放肆的我 時(shí)間: 2025-3-27 06:50
Computer-Aided Design and Pre-validation of Large Batches of DNA Assemblies,n the part sequence can cause assembly failures, incurring troubleshooting costs and project delays. As a result, the careful design and checking of the assembly plan is often a bottleneck of large assembly projects, and may require computational support. This chapter demonstrates the use of two fre作者: MAZE 時(shí)間: 2025-3-27 11:25
Computer-Aided Planning for the Verification of Large Batches of DNA Constructs,er, both require careful preparation to select restriction enzymes or DNA primers adapted to the expected constructs sequences. In projects involving manufacturing of large batches of synthetic constructs, the task can be tedious and error-prone. This chapter demonstrates the use of two free and ope作者: Cleave 時(shí)間: 2025-3-27 15:55 作者: Guaff豪情痛飲 時(shí)間: 2025-3-27 19:46
Steady-State Cell-Free Gene Expression with Microfluidic Chemostats,cell. Recent advances in microfluidic devices allowed cell-free reactions to run under nonequilibrium, steady-state conditions enabling the implementation of dynamic gene regulatory circuits in vitro. In this chapter, we present a detailed protocol to fabricate a microfluidic chemostat device which 作者: 茁壯成長 時(shí)間: 2025-3-27 23:20 作者: Lymphocyte 時(shí)間: 2025-3-28 04:25 作者: Urologist 時(shí)間: 2025-3-28 08:48
,Prediction of Cellular Burden with Host–Circuit Models,lting . is a widely recognized bottleneck in the design of robust circuits. In this tutorial we discuss the use of computational models that integrate gene circuits and the physiology of host cells. Through various use cases, we illustrate the power of host–circuit models to predict the impact of de作者: capsaicin 時(shí)間: 2025-3-28 11:41 作者: convulsion 時(shí)間: 2025-3-28 18:37 作者: DALLY 時(shí)間: 2025-3-28 20:36
Engineering Protein-Based Parts for Genetic Devices in Mammalian Cells,nously or exogenously driven regulatory pathways. While early genetic devices were engineered to be insulated from intracellular crosstalk, more recently the need of achieving dynamic control of cellular behavior has led to the development of smart interfaces that connect signal information (sensor)作者: 引導(dǎo) 時(shí)間: 2025-3-29 01:45
Computer-Aided Design and Pre-validation of Large Batches of DNA Assemblies,he assembly plan is often a bottleneck of large assembly projects, and may require computational support. This chapter demonstrates the use of two free and open-source web applications accelerating this task by automating genetic part design and simulating type-2S cloning to detect potential assembly issues.作者: exceptional 時(shí)間: 2025-3-29 04:02
Computer-Aided Planning for the Verification of Large Batches of DNA Constructs,manufacturing of large batches of synthetic constructs, the task can be tedious and error-prone. This chapter demonstrates the use of two free and open-source web applications providing fast and automated selection of enzymes and sequencing primers for DNA construct verification.作者: 吃掉 時(shí)間: 2025-3-29 10:07
Steady-State Cell-Free Gene Expression with Microfluidic Chemostats,tion of dynamic gene regulatory circuits in vitro. In this chapter, we present a detailed protocol to fabricate a microfluidic chemostat device which enables such an operation, detailing essential steps in photolithography, soft lithography, and hardware setup.作者: 手榴彈 時(shí)間: 2025-3-29 13:18
A Practical Step-by-Step Guide for Quantifying Retroactivity in Gene Networks,erizing retroactivity to restore the modular description of genetic modules. Additionally, we also discuss how retroactivity can be leveraged to quantify and maximize robustness to perturbations due to interconnection of genetic modules.作者: 悶熱 時(shí)間: 2025-3-29 16:20 作者: 確定 時(shí)間: 2025-3-29 19:59 作者: 出血 時(shí)間: 2025-3-30 02:40 作者: Melanocytes 時(shí)間: 2025-3-30 07:44
Madalena Chaves,Hidde de Jong is often mirrored in the eyes of these visitors. New teachers struggle particularly with this perception and will often leave the profession early if they cannot “get their class under control” (Wisneski, 2000). For this reason, teachers often need to perceive themselves as good “disciplinarians” l作者: Badger 時(shí)間: 2025-3-30 09:34 作者: Euphonious 時(shí)間: 2025-3-30 12:34 作者: 注意力集中 時(shí)間: 2025-3-30 20:27 作者: ANA 時(shí)間: 2025-3-30 21:55
Valentin Zulkowerhern ecosystems and dependent upon them contribute to the internal influences. We stress the importance of the complex web of relationships that characterizes northern ecosystems and the need for greater understanding of this complexity if we are to assess the consequences of external influences on 作者: 好色 時(shí)間: 2025-3-31 04:44
Deepti Vipin,Zoya Ignatova,Thomas E. Gorochowski old and new upcoming threats. The most crucial research questions are related to improving our understanding of the combined actions of pressures on ecosystem processes and species co-actions at regional scales and how they impact the human environment in return. The most crucial methodological def作者: FUME 時(shí)間: 2025-3-31 05:50 作者: GULP 時(shí)間: 2025-3-31 11:24
Mahmoud Khazim,Elisa Pedone,Lorena Postiglione,Diego di Bernardo,Lucia Maruccid on data from the ongoing Maastricht Cohort Study, the impact of various characteristics of working time arrangements (e.g., work schedules, working hours, overtime work, and hours control) both in the etiology and consequences of conflict between work and family life over time was investigated in 作者: Exuberance 時(shí)間: 2025-3-31 14:03 作者: Alveolar-Bone 時(shí)間: 2025-3-31 17:52 作者: insipid 時(shí)間: 2025-4-1 01:37 作者: 斗志 時(shí)間: 2025-4-1 05:10
