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標(biāo)題: Titlebook: Synthetic DNA; Methods and Protocol Randall A. Hughes Book 2017 Springer Science+Business Media, LLC, part of Springer Nature 2017 syntheti [打印本頁]

作者: Impacted    時(shí)間: 2025-3-21 19:45
書目名稱Synthetic DNA影響因子(影響力)




書目名稱Synthetic DNA影響因子(影響力)學(xué)科排名




書目名稱Synthetic DNA網(wǎng)絡(luò)公開度




書目名稱Synthetic DNA網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Synthetic DNA被引頻次




書目名稱Synthetic DNA被引頻次學(xué)科排名




書目名稱Synthetic DNA年度引用




書目名稱Synthetic DNA年度引用學(xué)科排名




書目名稱Synthetic DNA讀者反饋




書目名稱Synthetic DNA讀者反饋學(xué)科排名





作者: 草率女    時(shí)間: 2025-3-21 23:03

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Damien M. O’Hallorany and psychodrama tools into practice.Summarizes Moreno’s woThis open access book outlines the intersections between social work and the methods of sociometry and psychodrama. Different sections offer essential practice wisdom for both trauma-focused and trauma-informed experiential work for individ
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Jinsil Kim,Dong-Uk Kim,Kwang-Lae Hoecial contact and dysfunctional relationships during development are some of the most important contributors to psychiatric disorders over the lifetime, while some developmental disorders manifest as aberrant social behavior early in life. That the cause of mental illness is rooted in the brain was l
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Vishnu Krishnamurthy,Kai Zhang Ph.D.rom occasionally escaping from the present experience as you enter into a mental navigation mode. Sometimes spontaneously, others deliberately, your mind may move to a different time—you may see yourself running an experiment inspired by the chapter you just finished reading or you may imagine yours
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ideology, others were attracted to groups themselves and for that sake adopted the group’s ideas and were willing to engage in violence to defend them. Violence committed by radicalized individuals poses a growing threat. Ways to counter, or, more ideally, prevent, this development are needed. In th
作者: 干涉    時(shí)間: 2025-3-24 02:36
Book 2017ern biotechnology.? Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequences, the synthesis, assembly and cloning of synthetic DNA, and post-synthesis error reduction strategies.? Written in the highly successful .Methods in Molec
作者: 聲明    時(shí)間: 2025-3-24 08:32

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A Guide to Using , for Overlapping Assembly PCR Applicationsments using techniques such as loop-mediated isothermal amplification (LAMP). STITCHER is a web tool providing a central resource for researchers conducting all types of overlapping assembly PCR experiments with an intuitive interface for automated primer design that’s fast, easy to use, and freely available online.
作者: 共同給與    時(shí)間: 2025-3-25 00:19
A Guide to Using , for Overlapping Assembly PCR Applicationsand translational fusions, inserting mutations, making deletions, and PCR cloning. Overlapping PCR is also used for genotyping and in detection experiments using techniques such as loop-mediated isothermal amplification (LAMP). STITCHER is a web tool providing a central resource for researchers cond
作者: jet-lag    時(shí)間: 2025-3-25 05:13
Synthetic Gene Design Using Codon Optimization On-Line (COOL)y developed Codon Optimization On-Line (COOL) which is a web-based tool to provide multi-objective codon optimization functionality for synthetic gene design. COOL provides a simple and flexible interface for customizing codon optimization based on several design parameters such as individual codon
作者: Tailor    時(shí)間: 2025-3-25 10:29

作者: 平息    時(shí)間: 2025-3-25 13:49
Simple Cloning by Prolonged Overlap Extension-PCR with Application to the Preparation of Large-Size me, ligase, exonuclease, or recombinase in .. This technology can be applied to common . hosts (e.g., DH5α, JM109, TOP10, BL21(DE3)). The protocol includes three steps: (1) generate DNA insert and linear vector backbone by regular high-fidelity PCR, where these two DNA fragments contain 3′ and 5′ ov
作者: 集聚成團(tuán)    時(shí)間: 2025-3-25 19:51

作者: HOWL    時(shí)間: 2025-3-25 23:23
BASIC: A Simple and Accurate Modular DNA Assembly Method assembly and provides highly accurate DNA assembly with 99 % correct assemblies for four parts and 90 % correct assemblies for seven parts [1]. The BASIC standard defines a single entry vector for all parts flanked by the same prefix and suffix sequences and its idempotent nature means that the ass
作者: 愚蠢人    時(shí)間: 2025-3-26 04:03
Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotidesnostics, nanotechnology, and gene therapy. They are usually chemically synthesized. Here we describe an enzymatic method which enables us to synthesize pure oligonucleotides which can be up to several hundred long bases.
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The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesisom multiple off-products. This amplification step can cause stochastic sampling effects that propagate errors during the synthesis and lower the variability when applied towards the construction of randomized libraries. We present the method for polymerase step reaction (PSR), a simple DNA polymeras
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作者: 嫌惡    時(shí)間: 2025-3-27 06:36
Simultaneous Removal of Multiple DNA Segments by Polymerase Chain Reactionsce of certain restriction enzyme recognition sites. Recent development of restriction-free cloning approaches has greatly enhanced the flexibility and speed of molecular cloning. Most restriction-free cloning methods focus on DNA assembly. Much less work has been dedicated towards DNA removal. Here
作者: 無所不知    時(shí)間: 2025-3-27 09:36
Rapid Construction of Recombinant Plasmids by QuickStep-Cloninglasmid. It was designed specifically to address the major drawbacks of previously reported cloning methods. The fully optimized protocol allows for a seamless integration of a long DNA fragment into any position within a plasmid of choice, in a time-efficient and cost-effective manner, without the n
作者: 打算    時(shí)間: 2025-3-27 16:50
Book 2017eadily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...?.Authoritative and cutting-edge, .Synthetic DNA: Methods and Protocols. aims to help researchers further their research on manipulate DNA sequences..
作者: 圖表證明    時(shí)間: 2025-3-27 18:56

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作者: 積習(xí)已深    時(shí)間: 2025-3-28 04:52
Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineeringthe sheared fragments and recover and clone the library. The Shuffle Optimizer program and these protocols will be useful to anyone desiring to perform any of the nucleotide homology-dependent shuffling methods.
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Damien M. O’Halloranhin the fields of social work, psychodrama, the creative art therapies, group therapy, community organizing, and social activism.? ?.978-981-33-6344-1978-981-33-6342-7Series ISSN 2662-5490 Series E-ISSN 2662-5504
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作者: 失眠癥    時(shí)間: 2025-3-29 05:55

作者: 微不足道    時(shí)間: 2025-3-29 10:29
hin the fields of social work, psychodrama, the creative art therapies, group therapy, community organizing, and social activism.? ?.978-981-33-6344-1978-981-33-6342-7Series ISSN 2662-5490 Series E-ISSN 2662-5504
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Gene Deletion by Synthesis in Yeastare ligated into a single deletion cassette by ligase chain reaction, which is then amplified through two rounds of nested PCR to obtain sufficient quantities for yeast transformation. After removal of the artificial linkers, the deletion cassettes are transformed into wild-type diploid fission yeas
作者: 軍火    時(shí)間: 2025-3-29 23:53
1064-3745 utting-edge, .Synthetic DNA: Methods and Protocols. aims to help researchers further their research on manipulate DNA sequences..978-1-4939-8170-0978-1-4939-6343-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
作者: 詞匯記憶方法    時(shí)間: 2025-3-30 05:16
Marko Storch,Arturo Casini,Ben Mackrow,Tom Ellis,Geoff S. Baldwin
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作者: 小步走路    時(shí)間: 2025-3-30 13:18
and thus, the wider social meanings associated with that part of the intergenerational life course. The chapter presents a theoretical analysis of Chinese gerontology based on a critical reading of the work of Michel Foucault as applied to China and aging. It highlights the challenges and implicatio
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作者: Detonate    時(shí)間: 2025-3-31 02:59
Stephan Kirchmaier,Katharina Lust,Jochen WittbrodtLD response basic principles and the mechanisms involved in fMRI and fNIRS measurements related to brain function. We will then discuss the foundation of the social brain, based on the first studies, with one subject per data acquisition, to allow for understanding the new possibilities that hypersc




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