作者: 值得贊賞 時間: 2025-3-21 21:49 作者: 忘川河 時間: 2025-3-22 03:02
Eric Gottwald,Cordula Nies,Patrick Wuchter,Rainer Saffrich,Roman Truckenmüller,Stefan Giselbrecht作者: 過分自信 時間: 2025-3-22 06:06 作者: STEER 時間: 2025-3-22 08:46
Angela Jacobi,Philipp Rosendahl,Martin Kr?ter,Marta Urbanska,Maik Herbig,Jochen Guck作者: 聲音刺耳 時間: 2025-3-22 13:12
In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents,ilization agents. After extracting the contour of each cell, one can analyze the dynamics of cell “shapes” step-by-step, yielding various levels of information ranging from the principal mode of deformation, the persistence of deformation patterns, and the energy consumption by HSPC in the absence a作者: ineluctable 時間: 2025-3-22 18:41 作者: granite 時間: 2025-3-22 22:27
A Microcavity Array-Based 3D Model System of the Hematopoietic Stem Cell Niche,s with a diameter of 300?μm and a depth of up to 300?μm and arranged in a 634-cavity array. The polymer films are provided with 3?μm pores and thus allow perfusion of the culture medium. The microcavity arrays can be inserted into a microbioreactor where a closed circulation loop can be tightly cont作者: 果仁 時間: 2025-3-23 01:51
Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant,el system mimicking the extracellular matrix in vivo. The cells are encapsulated into the hydrogel network during polymerization, and a CXCL12 gradient is introduced in the enclosed chambers to trigger migration. Cell migration in the 3D network of the hydrogel is monitored by time-lapse microscopy.作者: 阻塞 時間: 2025-3-23 08:13 作者: Cpr951 時間: 2025-3-23 13:44
Assessing Cellular Hypoxic Status In Situ Within the Bone Marrow Microenvironment,uorescently labeled antibodies that recognize pimonidazole epitopes then enables the indirect assessment of the intracellular hypoxic status and its relationship to cell positioning within the complex tissue topography of the bone marrow.作者: 揉雜 時間: 2025-3-23 16:40
Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Flu we provide a detailed protocol for the characterization of hematopoietic stem and progenitor cells (HSPCs) in heterogeneous mobilized peripheral blood using RT-FDC and present a specific morpho-rheological fingerprint of HSPCs that allows to distinguish them from all other blood cell types.作者: 施加 時間: 2025-3-23 20:05 作者: 過度 時間: 2025-3-24 01:02 作者: 體貼 時間: 2025-3-24 05:01 作者: 動物 時間: 2025-3-24 08:46 作者: 個人長篇演說 時間: 2025-3-24 12:14
https://doi.org/10.1007/978-1-4939-9574-5Stem cell harvesting; Hematopoietic stem cells; Transplantation; Cell adhesion; Migration assays作者: Nutrient 時間: 2025-3-24 15:36
Gerd Klein,Patrick WuchterIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: Heretical 時間: 2025-3-24 19:47
978-1-4939-9576-9Springer Science+Business Media, LLC, part of Springer Nature 2019作者: remission 時間: 2025-3-25 01:59 作者: lacrimal-gland 時間: 2025-3-25 05:16 作者: Irrepressible 時間: 2025-3-25 07:47 作者: Muscularis 時間: 2025-3-25 14:26
Colony Formation: An Assay of Hematopoietic Progenitor Cells, the colonies provide preliminary information about the ability of progenitors to differentiate and proliferate. To allow colonies to grow to a size which facilitates accurate counting and identification, about 14?days of culture is sufficient. In certain situations also shorter periods may be used.作者: 顛簸地移動 時間: 2025-3-25 17:37 作者: 古代 時間: 2025-3-25 23:01
1064-3745 expertsThis book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers cell adhesion and migration assays作者: 少量 時間: 2025-3-26 01:00
Evolution of Peripheral Blood Stem Cell Transplantation, the bone marrow are as follows: harvesting blood stem cells is less painful for the donor, utilizes much less resources such as operating theater time and general anesthesia, and, above all, is associated with .. The latter has ultimately improved patient safety as a consequence of significantly sh作者: xanthelasma 時間: 2025-3-26 06:44
In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents,ifferent from the microscopy-based high-content screening relying on multiple staining, machine learning, and molecular-level perturbation, the proposed method sheds light on the “dynamics” of HSPC in the presence of extrinsic factors, including SDF1α and mobilization agents. A well-defined model of作者: malapropism 時間: 2025-3-26 09:19
Colony Formation: An Assay of Hematopoietic Progenitor Cells,ility to form colonies in a semisolid medium. The resulting colonies are consisting of more differentiated cells, and the number and the morphology of the colonies provide preliminary information about the ability of progenitors to differentiate and proliferate. To allow colonies to grow to a size w作者: 即席演說 時間: 2025-3-26 12:40 作者: 無辜 時間: 2025-3-26 20:46
Transmigration Assays for the Determination of Molecular Interactions Between Hematopoietic Stem Cehematopoiesis in the transplanted host, to proliferate, to differentiate, and to successfully repopulate the hematopoietic system of a transplanted host. Stimulating agents shown to induce the transmigration of HSPC were often later identified to play significant roles in mobilization of HSPC or the作者: DEAWL 時間: 2025-3-27 00:47 作者: 放大 時間: 2025-3-27 01:54
A Microcavity Array-Based 3D Model System of the Hematopoietic Stem Cell Niche,still far from being fully understood. One reason is that hematopoiesis is a multi-step maturation process leading to HSPC heterogeneity. Subpopulations of HSPCs can be identified by clonogenic assays or in serial transplantation experiments in mice following sublethal irradiation, but it is very co作者: Prosaic 時間: 2025-3-27 07:42 作者: 共棲 時間: 2025-3-27 10:36 作者: 高貴領(lǐng)導(dǎo) 時間: 2025-3-27 15:49
Assessing Cellular Hypoxic Status In Situ Within the Bone Marrow Microenvironment,hypothesized to be very limited. The hypoxic nature of HSC niches is proposed to play a fundamental role in the preservation of fundamental stem cell properties through the induction of a distinct glycolytic metabolic profile in HSCs. Thus, the capacity to determine oxygen levels or cellular oxygena作者: 蝕刻術(shù) 時間: 2025-3-27 20:04 作者: bile648 時間: 2025-3-27 22:00
Assessment of Proteolytic Activities in the Bone Marrow Microenvironment, marrow that has a strong influence on adhesive and chemotactic interactions of HSC with their niches. The increase of proteases during mobilization goes along with a decrease of endogenous protease inhibitors. Prominent members of the proteases involved in HSC mobilization belong to the families of作者: Mechanics 時間: 2025-3-28 04:10 作者: 細(xì)絲 時間: 2025-3-28 07:14 作者: 纖細(xì) 時間: 2025-3-28 13:12
Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantationd incidence of blood-related diseases. Age-related phenotypes have been widely studied by bone marrow transplantation experiments, where reconstitution of the transplanted cells is a direct measure of HSC activity. In this protocol we describe a competitive bone marrow transplantation assay to funct作者: 合法 時間: 2025-3-28 16:03
Zebrafish Xenografts for the In Vivo Analysis of Healthy and Malignant Human Hematopoietic Cells,ermore increasingly used for disease modeling and investigation of cancer biology. Zebrafish are particularly used for mutagenesis and small molecule screens, as well as for live imaging assays that provide unique opportunities to monitor cell behavior, both on a single cell and whole organism level作者: AFFIX 時間: 2025-3-28 20:08
Statistical and Mathematical Modeling of Spatiotemporal Dynamics of Stem Cells,e organization of hematopoietic stem and progenitor cells in the bone marrow environment. The current prominence of high-resolution and live-cell imaging data provides an unprecedented opportunity to study the spatiotemporal dynamics of these cells within their stem cell niche and learn more about a作者: 男學(xué)院 時間: 2025-3-29 00:38
Evolution of Peripheral Blood Stem Cell Transplantation,mental knowledge that culminated in the first successful attempts in blood stem cell transplantation. These efforts have spawned modern research into stem cell biology and the immune modulatory effects of allogeneic transplantations.作者: Kidnap 時間: 2025-3-29 04:08
Mobilization and Collection of Peripheral Blood Stem Cells in Adults: Focus on Timing and Benchmarkchestration of mobilization therapy, granulocyte colony-stimulating factor (G-CSF) application, and peripheral blood (PB) CD34. cell assessment. Moreover, strategies for prediction and performance assessment of the PBSC collection yield are discussed.作者: 我吃花盤旋 時間: 2025-3-29 09:40
Transmigration Assays for the Determination of Molecular Interactions Between Hematopoietic Stem Ce multi-well reusable transmigration assay using a small volume and low numbers of HSPC, allowing the simple and reproducible determination of HSPC transmigration capacity which enable researchers to obtain rapid answers at limited costs with high reliability.作者: 飛來飛去真休 時間: 2025-3-29 12:39
Zebrafish Xenografts for the In Vivo Analysis of Healthy and Malignant Human Hematopoietic Cells,esent a protocol for transplantation of healthy and malignant human hematopoietic cells into larval zebrafish; transplantation into adult zebrafish and possible advantages and limitations of the zebrafish compared to murine xenograft models are discussed.作者: AXIOM 時間: 2025-3-29 16:32 作者: cognizant 時間: 2025-3-29 22:02
Malignant Transformation by Ultraviolet and Ionizing Radiationscell lines, notably the C3H10T1/2 and BALB/3T3 cell systems. Although these cell lines are already immortal and aneuploid, they can be transformed to fully malignant, tumorigenic cells . by exposure to various physical and chemical carcinogens.作者: 危機 時間: 2025-3-29 23:59 作者: 該得 時間: 2025-3-30 06:32
Zhouyuan Zhangen that the mean climate of this region is generally shaped by the Indian monsoon and the unique high-elevation geographical features such as the Himalayas, the Western Ghats, the Tibetan Plateau and the adjoining Indian Ocean, Arabian Sea, and Bay of Bengal. This book also presents policy relevant 作者: 賞錢 時間: 2025-3-30 11:03
rperiode des AV-Knotens [74]. Im Gegensatz zu konventionellen Betabiockern jedoch verl?ngert es sowohl die rechts- und linksatriale wie auch die ventrikul?re Refrakt?rphase. Diese einzigartige Verbindung von Klasse-II-und Klasse-III-Wirkung ist von besonderem Vorteil bei der Behandlung von supravent作者: gruelling 時間: 2025-3-30 12:55
Femur Fracturesxcellent, and the complications are minimal. Patients whose resuscitation fails to correct critical parameters such as metabolic acidosis, hypothermia, and coagulopathy are not safe candidates for intramedullary definitive stabilization of the femur and should be considered candidates for damage con作者: palliative-care 時間: 2025-3-30 20:32 作者: 清醒 時間: 2025-3-31 00:36
Global Justice? Contesting NGOization: Knowledge Politics and Containment in Antiglobalization Netwoerrogating “alternatives to globalization” advocacy positions advanced by nongovernmental actors ostensibly committed to social transformation, I argue that critical attention to texts and actual practices reveals that many NGOs are tied to what I refer to as an ideology of pragmatism which normaliz作者: 冒失 時間: 2025-3-31 01:39 作者: 滔滔不絕地講 時間: 2025-3-31 06:34
2634-6494 e generation of new ideas through an erotic but non-physical connection between two men, and it sees Owen as both a product and a continuation of this Wildean tradition.978-1-137-55064-4Series ISSN 2634-6494 Series E-ISSN 2634-6508
