標(biāo)題: Titlebook: Stable Isotope Probing; Methods and Protocol Marc G. Dumont,Marcela Hernández García Book 2019 Springer Science+Business Media, LLC, part o [打印本頁] 作者: 可憐 時(shí)間: 2025-3-21 17:13
書目名稱Stable Isotope Probing影響因子(影響力)
作者: 沉著 時(shí)間: 2025-3-21 21:03 作者: 大門在匯總 時(shí)間: 2025-3-22 03:42
Quantification of Methanogenic Pathways Using Stable Carbon Isotopic Signatures,c composition of these compounds by isotope ratio mass spectrometry are described in this chapter. The results are very helpful in evaluating the activity of the methanogens involved in each methanogenic pathway as well as those of other biological pathways with different fractionation factors.作者: 的闡明 時(shí)間: 2025-3-22 07:15 作者: Watemelon 時(shí)間: 2025-3-22 11:45
Microbial Taxon-Specific Isotope Incorporation with DNA Quantitative Stable Isotope Probing,a substrate, as it also allows for measuring that assimilation for each taxon within a given microbial community. Here, we describe an analysis process necessary to determine atom fraction excess of a heavy stable isotope added to an environmental sample for a given taxon’s DNA.作者: moribund 時(shí)間: 2025-3-22 14:25
,Profiling of Active Microorganisms by Stable Isotope Probing—Metagenomics, on the key functional genes in specific metabolic pathways and their relative abundances. In this chapter, we provide a general background on the application of the SIP-metagenomics approach in microbial ecology and a workflow for the analysis of metagenomic datasets using the most up-to-date bioinformatics tools.作者: escalate 時(shí)間: 2025-3-22 20:59 作者: semiskilled 時(shí)間: 2025-3-22 23:50 作者: 善辯 時(shí)間: 2025-3-23 05:21 作者: 無畏 時(shí)間: 2025-3-23 06:41
Stable Isotope Probing of Microbial Phospholipid Fatty Acids in Environmental Samples,, followed by lipid separation based on solid-phase extraction and an alkaline methylation. Finally, we provide a protocol for the carbon stable isotope measurements of the extracted fatty acid methyl esters (FAMEs) by gas chromatograph–isotope ratio mass spectrometer (GC-IRMS) and calculation of concentration and .C. values.作者: 音樂等 時(shí)間: 2025-3-23 13:39 作者: 虛弱的神經(jīng) 時(shí)間: 2025-3-23 17:23 作者: CRP743 時(shí)間: 2025-3-23 18:19 作者: 折磨 時(shí)間: 2025-3-23 23:54
Targeted Metatranscriptomics of Soil Microbial Communities with Stable Isotope Probing,ion of RNA-SIP with metatranscriptomic analysis enhances the detection and identification of mRNA from target microorganisms. In this chapter we provide a protocol for RNA-SIP, mRNA enrichment, and mRNA preparation for high-throughput sequencing using an example of targeting methanotrophs in rice field soil.作者: avarice 時(shí)間: 2025-3-24 03:24 作者: narcissism 時(shí)間: 2025-3-24 09:44 作者: novelty 時(shí)間: 2025-3-24 10:56
DNA-Based Stable Isotope Probing,ten remain elusive. DNA-based stable isotope probing (DNA-SIP) provides a powerful means to establish a direct link between biogeochemical processes and the taxonomic identities of active microorganisms involved in the processes. Combined with high-throughput sequencing, it significantly aids in dec作者: Flirtatious 時(shí)間: 2025-3-24 18:04
RNA Stable Isotope Probing (RNA-SIP),vironmental samples. Employing stable isotopes of common elements such as carbon and nitrogen, RNA-SIP exploits an increase in the buoyant density of RNA caused by the active metabolism and incorporation of heavier mass isotopes into the RNA after cellular utilization of labeled substrates pulsed in作者: mucous-membrane 時(shí)間: 2025-3-24 19:48 作者: aggravate 時(shí)間: 2025-3-25 02:53 作者: Meditate 時(shí)間: 2025-3-25 05:30
Chip-SIP: Stable Isotope Probing Analyzed with rRNA-Targeted Microarrays and NanoSIMS,e isotopes (.C, .N, and .O) simultaneously. This method uses a high-density microarray to separate taxon-specific 16S (or 18S) rRNA genes and a high sensitivity magnetic sector secondary ion mass spectrometer (SIMS) to determine the relative isotope incorporation of the rRNA at each probe location. 作者: perimenopause 時(shí)間: 2025-3-25 09:12 作者: 換話題 時(shí)間: 2025-3-25 13:34
Stable Isotope-Labeled Single-Cell Raman Spectroscopy Revealing Function and Activity of Environmen remain largely elusive because the vast majority of them are yet uncultured. Recent achievements in single cell stable isotope-labeled Raman spectroscopy enable direct investigation of function and activity of individual microbes in complex environmental communities. Here, this protocol describes a作者: 最小 時(shí)間: 2025-3-25 19:53
Data Analysis for DNA Stable Isotope Probing Experiments Using Multiple Window High-Resolution SIP,ibe the analysis of sequencing data using the multiple window high-resolution DNA-SIP method (MW-HR-SIP). MW-HR-SIP has improved accuracy over other methods and is easily implemented on the statistical platform R. We also discuss key experimental parameters to consider when designing DNA-SIP experim作者: 不可思議 時(shí)間: 2025-3-25 20:40 作者: esculent 時(shí)間: 2025-3-26 02:18
Microbial Taxon-Specific Isotope Incorporation with DNA Quantitative Stable Isotope Probing, labeled with a heavy isotope..The laboratory protocol for qSIP is nearly identical to that for conventional stable isotope probing, with two key additions: (1) in qSIP, qPCR measurements are conducted on each density fraction recovered after isopycnic separation, and (2) in qSIP, multiple density f作者: ANTH 時(shí)間: 2025-3-26 05:11 作者: 破布 時(shí)間: 2025-3-26 11:55 作者: 屈尊 時(shí)間: 2025-3-26 12:42
Stable Isotope Probing Techniques and Methodological Considerations Using 15N,le isotope probing was so far limited because of technical challenges related to the relative rarity of nitrogen in nucleic acids and proteins compared to carbon, and because of its absence in lipids. However, the recent adoption of high-throughput sequencing and statistical modelling methods to SIP作者: 以煙熏消毒 時(shí)間: 2025-3-26 18:42 作者: Androgen 時(shí)間: 2025-3-26 21:23
Method Development for DNA and Proteome SIP Analysis of Activated Sludge for Anaerobic Dichloromethan be used as the sole source of energy and organic carbon by anaerobic microorganisms and be transformed to benign end products such as acetate, formate, and bicarbonate. However, knowledge around the phylogenetic diversity of anaerobic microorganisms capable of DCM metabolism is limited. The genes作者: 我不死扛 時(shí)間: 2025-3-27 03:37 作者: prosthesis 時(shí)間: 2025-3-27 06:49
Stable Isotope Probing of Microbiota Structure and Function in the Plant Rhizosphere, in root exudate assimilation and those involved in soil organic matter degradation, and (b) the study of the impact of plants via root exudates on the in situ expression of microbial functions. By incubating plants under .CO., fresh carbon exuded by the plant will be labeled and hence the microbial作者: progestin 時(shí)間: 2025-3-27 11:50
978-1-4939-9723-7Springer Science+Business Media, LLC, part of Springer Nature 2019作者: TAP 時(shí)間: 2025-3-27 15:28 作者: 大火 時(shí)間: 2025-3-27 21:19
Methods in Molecular Biologyhttp://image.papertrans.cn/s/image/875456.jpg作者: unstable-angina 時(shí)間: 2025-3-27 23:15 作者: dendrites 時(shí)間: 2025-3-28 04:23
Stable Isotope Probing of Microorganisms in Environmental Samples with H218O,strate for all microorganisms and replication is required for DNA to become labeled with .O. By measuring how much the DNA of each taxon becomes enriched with .O when an environmental sample is incubated with H.O, it is feasible to quantify that population’s DNA replication rate, which is a proxy for growth.作者: IRK 時(shí)間: 2025-3-28 07:54 作者: 鳴叫 時(shí)間: 2025-3-28 14:05
Marc G. Dumont,Marcela Hernández GarcíaIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: PARA 時(shí)間: 2025-3-28 16:51
mpiled and presented by Jean Seznec, this anthology helps th.Chance ordained that Denis Diderot (1713-1784) was not only a philosopher, playwright and writer, but also a .salonnier.. In other words, an art critic. In 1759, his friend Grimm entrusted him with a project that forced him to acquire "tho作者: 機(jī)械 時(shí)間: 2025-3-28 19:29 作者: VAN 時(shí)間: 2025-3-29 00:18
Zhongjun Jia,Weiwei Cao,Marcela Hernández Garcíaic. In 1759, his friend Grimm entrusted him with a project that forced him to acquire "thoughtful notions concerning painting and sculpture" and to refine "art terms, so familiar in his words yet so vague in his mind"...Diderot wrote artistic reviews of exhibitions – Salons – that were organized bi-作者: 起草 時(shí)間: 2025-3-29 03:50 作者: 劇毒 時(shí)間: 2025-3-29 07:29
Andrea Watzinger,Rebecca Hood-Nowotnympiled and presented by Jean Seznec, this anthology helps th.Chance ordained that Denis Diderot (1713-1784) was not only a philosopher, playwright and writer, but also a .salonnier.. In other words, an art critic. In 1759, his friend Grimm entrusted him with a project that forced him to acquire "tho作者: 燦爛 時(shí)間: 2025-3-29 13:50
Martin Taubertic. In 1759, his friend Grimm entrusted him with a project that forced him to acquire "thoughtful notions concerning painting and sculpture" and to refine "art terms, so familiar in his words yet so vague in his mind"...Diderot wrote artistic reviews of exhibitions – Salons – that were organized bi-作者: harpsichord 時(shí)間: 2025-3-29 19:23
Xavier Mayali,Peter K. Weber,Erin Nuccio,Jory Lietard,Mark Somoza,Steven J. Blazewicz,Jennifer Pett-ic. In 1759, his friend Grimm entrusted him with a project that forced him to acquire "thoughtful notions concerning painting and sculpture" and to refine "art terms, so familiar in his words yet so vague in his mind"...Diderot wrote artistic reviews of exhibitions – Salons – that were organized bi-作者: 宣誓書 時(shí)間: 2025-3-29 22:02
Quan Yuanic. In 1759, his friend Grimm entrusted him with a project that forced him to acquire "thoughtful notions concerning painting and sculpture" and to refine "art terms, so familiar in his words yet so vague in his mind"...Diderot wrote artistic reviews of exhibitions – Salons – that were organized bi-作者: 雪崩 時(shí)間: 2025-3-30 01:58 作者: 滋養(yǎng) 時(shí)間: 2025-3-30 05:22 作者: 陳舊 時(shí)間: 2025-3-30 08:31 作者: osculate 時(shí)間: 2025-3-30 15:53 作者: 天真 時(shí)間: 2025-3-30 20:32 作者: Sarcoma 時(shí)間: 2025-3-30 21:34 作者: Peak-Bone-Mass 時(shí)間: 2025-3-31 03:35
Roey Angel, appears for the first time in a monograph form.Examples frThis book gives a detailed and self-contained introduction into the theory of spectral functions, with an emphasis on their applications to quantum field theory. All methods are illustrated with applications to specific physical problems fr作者: 舉止粗野的人 時(shí)間: 2025-3-31 08:36 作者: 熱情的我 時(shí)間: 2025-3-31 09:59
Miao Hu,Matthew Lee,Ling Zhong,Michael J. Manefieldta-function, the zeta-function of selfadjoint elliptic second order differential operators (both positive and semidefinite), relations between the zeta-function residues and the heat kernel coefficients. Among less standard issues, a brief description of the spectral density of operators, relation b作者: 勉強(qiáng) 時(shí)間: 2025-3-31 13:50
Severin Weis,Sylvia Schnell,Markus Egertn forecasting, but not yet in today’s laminar, coarse-resolution ocean components of models of the climate system. However, advances in high performance computing are likely to change this in a near future, as the next decade should see the use of eddying models to become more and more frequent in t作者: 有危險(xiǎn) 時(shí)間: 2025-3-31 17:45 作者: subacute 時(shí)間: 2025-3-31 21:48 作者: profligate 時(shí)間: 2025-4-1 04:45
DNA-Based Stable Isotope Probing, essential prerequisite for a successful DNA-SIP experiment is the identification, with confidence, of isotopically enriched .C-DNA, of which the amount is generally too low to allow for the direct measurement of .C atomic percent of nucleic acid. The .C labeling can be readily identified in the fra
