標(biāo)題: Titlebook: Spermatogonial Stem Cells; Methods and Protocol Jon M. Oatley,Brian P. Hermann Book 2023 The Editor(s) (if applicable) and The Author(s), u [打印本頁] 作者: JOLT 時間: 2025-3-21 19:34
書目名稱Spermatogonial Stem Cells影響因子(影響力)
作者: Esophagus 時間: 2025-3-21 22:43 作者: 運(yùn)動性 時間: 2025-3-22 02:29
978-1-0716-3141-6The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines作者: Enrage 時間: 2025-3-22 05:07 作者: 雜役 時間: 2025-3-22 08:48
Jon M. Oatley,Brian P. HermannIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contain key notes and implementation advice from the experts作者: Fretful 時間: 2025-3-22 15:26
Viral Transduction of Mammalian Spermatogonial Stem Cells,mising emerging tools for in vivo SSC transduction that are less invasive, compared to lentivirus, since AAV DNA is not integrated into the host genome and the host genome remains intact. In this chapter, we describe protocols using lentiviral and adeno-associated viral vectors to transduce SSCs in vitro and vivo, respectively.作者: admission 時間: 2025-3-22 20:22 作者: Genistein 時間: 2025-3-23 00:08 作者: Manifest 時間: 2025-3-23 03:55
,Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell: Part 2—Computational Analysis o genomic regions. In addition, we describe Chromatin State Discovery and Characterization (ChromHMM) methodology to integrate data from these individual analyses, plus that from RNA-seq analysis of gene expression, to obtain the most comprehensive overall assessment of epigenetic programming associa作者: optional 時間: 2025-3-23 08:50 作者: ATRIA 時間: 2025-3-23 12:55 作者: 自制 時間: 2025-3-23 14:11
Lineage Tracing of Spermatogonial Stem Cells Within the Male Germline,leveraging transgenic technologies have prevailed as the preferred avenue for modern lineage tracing. This chapter will discuss current protocols for effective genetic lineage tracing and address applications of this technology, considerations when designing lineage tracing experiments, and the meth作者: 正論 時間: 2025-3-23 20:17 作者: spinal-stenosis 時間: 2025-3-23 23:17 作者: Delectable 時間: 2025-3-24 02:46
Makiko Yamada,Yanyun Luo,Marco Seandel an intricate and bold combination of facts and methods from most diverse fields, in order to perform functions and operations that fit into an overall technolo978-3-642-47551-1978-3-642-47547-4Series ISSN 0930-8989 Series E-ISSN 1867-4941 作者: cumulative 時間: 2025-3-24 09:20
Gunapala Shetty?ort has been devoted to develop methods and algorithms able to solve the problem even in its more di?cult and large instances. The lectures have been given by eminent scholars, who contributed978-3-642-11338-3978-3-642-11339-0Series ISSN 0075-8434 Series E-ISSN 1617-9692 作者: ELUDE 時間: 2025-3-24 11:23
Julien M. D. Legrand,Robin M. Hobbs?ort has been devoted to develop methods and algorithms able to solve the problem even in its more di?cult and large instances. The lectures have been given by eminent scholars, who contributed978-3-642-11338-3978-3-642-11339-0Series ISSN 0075-8434 Series E-ISSN 1617-9692 作者: 固執(zhí)點(diǎn)好 時間: 2025-3-24 16:00
Nathan C. Law?ort has been devoted to develop methods and algorithms able to solve the problem even in its more di?cult and large instances. The lectures have been given by eminent scholars, who contributed978-3-642-11338-3978-3-642-11339-0Series ISSN 0075-8434 Series E-ISSN 1617-9692 作者: Stagger 時間: 2025-3-24 22:29 作者: hardheaded 時間: 2025-3-24 23:33
Yasunari Seita,Young Sun Hwang,Kotaro Sasakitechnology and will provide new alternatives in information processing and transmission. The conception of new devices, in general, requires an intricate and bold combination of facts and methods from most diverse fields, in order to perform functions and operations that fit into an overall technolo作者: 軍火 時間: 2025-3-25 05:51
Vera van Melis,Lorena Roa-de la Cruz,Brian P. Hermannand (2) the interaction distance raised to some power greater than or equal to unity. Since power density is power per unit area, the efficiency of any nonlinear interaction can be enhanced by reducing the cross-sectional area of the interacting beams. For plane waves this can be achieved by focusin作者: Cardiac 時間: 2025-3-25 08:31 作者: Hallmark 時間: 2025-3-25 14:32 作者: OTHER 時間: 2025-3-25 17:08 作者: 巫婆 時間: 2025-3-25 20:03
Sarah K. Munyoki,Kyle E. Orwigzed to be of main relevance in engineering, economics, and other applied sciences, so that a great lot of e?ort has been devoted to develop methods and algorithms able to solve the problem even in its more di?cult and large instances. The lectures have been given by eminent scholars, who contributed作者: Silent-Ischemia 時間: 2025-3-26 00:43
Spermatogonial Transplantation,es the assay endpoint. Using the mouse as the model system, we here describe the basic techniques of spermatogonial transplantation and provide practical guidance to successfully carry out this technique and to interpret data generated.作者: Anthrp 時間: 2025-3-26 07:19
Perspectives: Approaches for Studying Livestock Spermatogonia,ant differences exist between mice and higher order mammals. In this chapter, we briefly discuss distinguishing aspects of testicular anatomy and the spermatogenic lineage in livestock and critical considerations for studying spermatogonial stem cell biology in these species.作者: CODA 時間: 2025-3-26 09:19 作者: 比喻好 時間: 2025-3-26 15:53
,Introduction: The Why’s and How’s for Studying Spermatogenesis and Spermatogonial Stem Cells,ying spermatogenesis is important, including a high-level overview of spermatogonia and SSCs, and make the case for a critical need for use of stringent definitions for SSCs and experimental platforms that allow for clear distinction of the multiple types of spermatogonia that exist in testes of mammals.作者: 條約 時間: 2025-3-26 16:56
Optimization of Testicular Fixation-Embedding Techniques for Improved Evaluation of Mammalian Spermsis of spermatogonial morphology and function, involving fixation of mammalian?testicular tissue in glutaraldehyde and embedding in plastic resin. These techniques have proven to be effective in light microscopy studies.作者: Modicum 時間: 2025-3-26 21:06
Primary Cultures of Spermatogonia and Testis Cells,plantation of the cultured mouse and rat SSCs. Using the in vitro SSC culture systems, elucidation of stem cell biology can be advanced that significantly advances our understanding of spermatogenesis and male fertility.作者: 負(fù)擔(dān) 時間: 2025-3-27 01:56 作者: condemn 時間: 2025-3-27 07:11
Defining Gene Function in Spermatogonial Stem Cells Through Conditional Knockout Approaches,oups, and treatment regimens for inducible knockout models and provide advice for verifying and improving conditional knockout efficiency. This resource can be beneficial to those aiming to develop conditional knockout models for the study of SSC development and postnatal function.作者: 使隔離 時間: 2025-3-27 11:01
Book 2023critical analysis of spermatogonial subsets including the rare spermatogonial stem cells (SSCs) pool.? Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step作者: 悲痛 時間: 2025-3-27 16:18
1064-3745 tion advice from the expertsThis volume explores the latest techniques used in the field of mammalian germ cell biology to study key experimental approaches and perspectives for critical analysis of spermatogonial subsets including the rare spermatogonial stem cells (SSCs) pool.? Written in the high作者: 公司 時間: 2025-3-27 20:35 作者: esthetician 時間: 2025-3-28 00:06 作者: 香料 時間: 2025-3-28 02:26
Single-Molecule Fluorescence In Situ Hybridization for Spatial Detection of mRNAs in Sections of Mamolecule resolution while preserving spatial context, and thus, is a useful tool for examining gene expression in biological systems. In particular, the growing reliance on single-cell RNA sequencing (scRNA-seq) to explore cellular heterogeneity has reinvigorated this approach as a validation tool t作者: Flirtatious 時間: 2025-3-28 08:08 作者: 魔鬼在游行 時間: 2025-3-28 13:31
,Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell—Part 1: Epigenomics Assays, cell fate and function. Here, protocols are provided for four different epigenomic methods including whole-genome bisulfite sequencing (WGBS) to assess DNA methylation patterns, chromatin immunoprecipitation-sequencing (ChIP-seq) to assess genomic patterns of either specific histone modifications o作者: bypass 時間: 2025-3-28 17:16 作者: MUT 時間: 2025-3-28 21:51
Primary Cultures of Spermatogonia and Testis Cells,o study the biology of SSCs as related to spermatogenesis, an in vitro, long-term expansion system of SSCs constitutes an ideal tool. In this chapter, we describe a robust culture system for mouse and rat SSCs in vitro. In the presence of GDNF, GFRα1, and bFGF, SSCs maintained on STO feeder layers w作者: Interregnum 時間: 2025-3-29 00:31
Reconstitution of Human Prospermatogonial Development from Human-Induced Pluripotent Stem Cells,ant materials. Reconstitution of human germ cell development from pluripotent stem cells in vitro would provide critical insight into the etiology of various reproductive conditions and disorders, including infertility..Recently, we reported the in vitro reconstitution of human prospermatogonial dev作者: Kidnap 時間: 2025-3-29 04:53 作者: 使無效 時間: 2025-3-29 07:22 作者: 防御 時間: 2025-3-29 13:04 作者: judiciousness 時間: 2025-3-29 18:35 作者: 滔滔不絕的人 時間: 2025-3-29 22:57 作者: Trypsin 時間: 2025-3-30 01:44 作者: 悲觀 時間: 2025-3-30 05:31
Defining Gene Function in Spermatogonial Stem Cells Through Conditional Knockout Approaches,Much of our current understanding regarding the molecular mechanisms underlying SSC activity is derived from studies using conditional knockout mouse models. Here, we provide a guide for the selection and use of mouse strains to develop conditional knockout models for the study of SSCs, as well as t作者: 輕信 時間: 2025-3-30 11:45 作者: insert 時間: 2025-3-30 13:18 作者: WAIL 時間: 2025-3-30 16:43
Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells,undation of spermatogenesis and can either self-renew, producing more SSCs, or differentiate to initiate spermatogenesis and produce sperm. The biological potential of SSCs to produce and maintain spermatogenesis makes them a promising tool for the treatment of male infertility. However, translating作者: 鉆孔 時間: 2025-3-30 22:59
,Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell—Part 1: Epigenomics Assays, plus computational methodology to integrate data from those four methodologies using Chromatin State Discovery and Characterization (ChromHMM) to obtain the most comprehensive overall assessment of epigenetic programming.作者: 鄙視讀作 時間: 2025-3-31 01:10 作者: 能量守恒 時間: 2025-3-31 07:32
Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells,togenesis in a transplant assay, there is no equivalent routine and accessible bioassay to test monkey and human SSCs or replicate their functions in vitro. This chapter describes progress characterizing, isolating, culturing, and transplanting SSCs in higher primates.作者: 上下倒置 時間: 2025-3-31 11:38
Bulk and Single-Cell RNA-Seq Analyses for Studies of Spermatogonia,workflows for the analysis of bulk RNA-seq from aggregate samples of hundreds to millions of cells and single-cell RNA-seq from individual cells. We also provide detailed protocols for using the R packages DESeq2 and Seurat, important parameters for successful implementation, and considerations for drawing conclusions from the results.作者: BATE 時間: 2025-3-31 16:26 作者: Albumin 時間: 2025-3-31 18:18
Connor Cason,Tessa Lorded in many direc- tions and its techniques have intruded upon many areas of both fundamental and practical interest. The field itself has been enriched with many new phe- nomena and concepts that have further extended its scope and strengthened its connection with other areas. As a consequence, it i作者: 侵略 時間: 2025-3-31 22:54
Vera van Melis,Lorena Roa-de la Cruz,Brian P. Hermannearch [1–2], to the generation of new frequencies [1], and most recently to all-optical signal processing [3]. In general, nonlinear optical interactions occur whenever the optical fields associated with one or more laser beams propagating in a material are large enough to produce polarization field作者: Collected 時間: 2025-4-1 02:35 作者: 郊外 時間: 2025-4-1 09:21
Makiko Yamada,Yanyun Luo,Marco Seandeled in many direc- tions and its techniques have intruded upon many areas of both fundamental and practical interest. The field itself has been enriched with many new phe- nomena and concepts that have further extended its scope and strengthened its connection with other areas. As a consequence, it i作者: Canyon 時間: 2025-4-1 11:38
Oleksandr Kirsanov,Christopher B. Geyerearch [1–2], to the generation of new frequencies [1], and most recently to all-optical signal processing [3]. In general, nonlinear optical interactions occur whenever the optical fields associated with one or more laser beams propagating in a material are large enough to produce polarization field作者: 挫敗 時間: 2025-4-1 14:24
Gunapala Shetty on Nonlinear Optimization held in Cetraro, Italy, from July 1 to 7, 2007. The Nonlinear Optimization problem of main concern here is the problem n of determining a vector of decision variables x ? R that minimizes (ma- n mizes) an objective function f(·): R ? R,when x is restricted to belong n to s作者: 詢問 時間: 2025-4-1 18:37
