標題: Titlebook: Signal Transduction Protocols; Robert C. Dickson,Michael D. Mendenhall Book 20042nd edition Humana Press 2004 [打印本頁] 作者: CULT 時間: 2025-3-21 17:03
書目名稱Signal Transduction Protocols影響因子(影響力)
書目名稱Signal Transduction Protocols影響因子(影響力)學科排名
書目名稱Signal Transduction Protocols網(wǎng)絡公開度
書目名稱Signal Transduction Protocols網(wǎng)絡公開度學科排名
書目名稱Signal Transduction Protocols被引頻次
書目名稱Signal Transduction Protocols被引頻次學科排名
書目名稱Signal Transduction Protocols年度引用
書目名稱Signal Transduction Protocols年度引用學科排名
書目名稱Signal Transduction Protocols讀者反饋
書目名稱Signal Transduction Protocols讀者反饋學科排名
作者: 做方舟 時間: 2025-3-22 00:00 作者: 傀儡 時間: 2025-3-22 00:57
Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation,quences are associated with the protein of interest in vivo. The ChIP method described here is intended for studying protein-DNA association in the budding yeast ., but it can be easily implemented in other cell types, including fly, mammalian, and plant cells.作者: watertight, 時間: 2025-3-22 04:43 作者: 招人嫉妒 時間: 2025-3-22 09:19 作者: Entrancing 時間: 2025-3-22 16:42 作者: hemoglobin 時間: 2025-3-22 17:09
Techniques in Protein Methylation,ine methyltransferases to illustrate different approaches that have been developed to assess protein methylation. Both in vivo and in vitro methylation techniques are described, and the use of small molecule inhibitors of protein methylation will be demonstrated.作者: 人充滿活力 時間: 2025-3-23 00:08
Making Protein Immunoprecipitates, Immunoprecipitation and related methods of affinity purification are routinely used to assess binding partner interactions and enzyme activity in addition to the size of a protein, rates of protein synthesis and turnover, and protein abundance, thus making it a mainstay of a wide variety of protoco作者: immunity 時間: 2025-3-23 03:50 作者: 導師 時間: 2025-3-23 06:18
Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets,tions, providing a good strategy to monitor molecular responses induced by the activation or inactivation of specific signaling pathways. In this chapter, optimized protocols for 2-DE using extracts from tissue culture are provided. Protocols for in-gel digestion of gel-resolved proteins, which allo作者: 功多汁水 時間: 2025-3-23 11:01 作者: SHRIK 時間: 2025-3-23 17:52
Determining Protein Half-Lives,ing the turnover rate of a protein is often the first step in assessing whether or not the function of a protein is regulated by proteolysis under specific physiological conditions. Over the years, procedures to determine the half-life of proteins in cultured eukaryotic cells have been well-establis作者: 慎重 時間: 2025-3-23 20:35 作者: 颶風 時間: 2025-3-23 22:23 作者: fodlder 時間: 2025-3-24 05:48 作者: forthy 時間: 2025-3-24 06:46
Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation,c regions in the context of intact cells. In this method, cells are first treated with formaldehyde to crosslink protein-protein and protein-DNA complexes .. Next, the crosslinked chromatin is sheared by sonication to generate small chromatin fragments, and the fragments associated with the protein 作者: 中世紀 時間: 2025-3-24 12:22 作者: eulogize 時間: 2025-3-24 18:17
Nucleocytoplasmic Glycosylation, O-GlcNAc, in metazoans. This modification is dynamic and responsive to numerous stimuli and conditions, suggesting an important role in many regulatory pathways. Moreover, the O-GlcNAc modification seems to compete with phosphorylation for sites of attachment, indicating a reciprocal relationship with phosph作者: Adj異類的 時間: 2025-3-24 20:40
Techniques in Protein Methylation,hanging the primary sequence of a peptide so that it can encode more information. This common posttranslational modification is implicated in the regulation of a variety of processes including protein trafficking, transcription and protein-protein interactions. In this chapter, we will use the argin作者: 紳士 時間: 2025-3-25 00:05 作者: 壕溝 時間: 2025-3-25 06:37 作者: 機械 時間: 2025-3-25 07:34 作者: 后天習得 時間: 2025-3-25 12:45 作者: 我說不重要 時間: 2025-3-25 15:52 作者: 浪費物質 時間: 2025-3-25 20:51 作者: 單挑 時間: 2025-3-26 00:52 作者: 變形詞 時間: 2025-3-26 05:17 作者: vasculitis 時間: 2025-3-26 12:14 作者: Monocle 時間: 2025-3-26 12:52
Assaying Phosphoinositide Phosphatases, and regulate the actions of phosphoinositide kinases. We describe herein two rapid and simple assays for characterizing phosphoinositide phosphatases that can be used to provide a basis for understanding the activity and specificity of these enzymes.作者: BOAST 時間: 2025-3-26 20:52
Assaying Phospholipase A2 Activity,tivity in cell lysates from other PLA.s. sPLA. activity is monitored using a fluorimetric assay that measures the continuous calcium-dependent formation of albumin-bound pyrene fatty acid from the . position of phosphatidylglycerol.作者: Corporeal 時間: 2025-3-26 23:03 作者: 秘方藥 時間: 2025-3-27 02:35
Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets,ter, optimized protocols for 2-DE using extracts from tissue culture are provided. Protocols for in-gel digestion of gel-resolved proteins, which allow protein identification by mass spectrometry are also discussed.作者: 可憎 時間: 2025-3-27 06:58
Determining Protein Half-Lives,cific physiological conditions. Over the years, procedures to determine the half-life of proteins in cultured eukaryotic cells have been well-established. This chapter describes in detail the two most frequently used methods, pulse-chase analysis and cycloheximide blocking, to determine a protein’s half-life in yeast and cultured mammalian cells.作者: 討人喜歡 時間: 2025-3-27 13:21 作者: 膽汁 時間: 2025-3-27 17:15
Methods in Molecular Biologyhttp://image.papertrans.cn/s/image/867101.jpg作者: badinage 時間: 2025-3-27 19:24
Signal Transduction Protocols978-1-59259-816-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 吹氣 時間: 2025-3-28 00:01 作者: 不能約 時間: 2025-3-28 05:53
Robert C. Dickson,Michael D. MendenhallIncludes supplementary material: 作者: DEMN 時間: 2025-3-28 08:47 作者: epidermis 時間: 2025-3-28 12:38
Elaine A. Elion,Yunmei Wangir answers, which have been analyzed using discourse analysis methods, to identify, classify, and connect the main themes presented by respondents. Although the topics raised in various questions were quite different, it was possible to classify the respondents by their general attitude regarding th作者: crockery 時間: 2025-3-28 18:23
Maofu Fu,Chenguang Wang,Xueping Zhang,Richard G. Pestellhe growth of the attractiveness of the city of Oradea. The research methodology consisted of data processing and analysis (from 2007 until November 2019) provided by the National Institute of Statistics from Romania regarding tourist movement in the city of Oradea. The selected quantitative indicato作者: Trypsin 時間: 2025-3-28 22:22
Yukihito Kabuyama,Kirsi K. Polvinen,Katheryn A. Resing,Natalie G. Ahncreate new defence strategies, capabilities and mechanisms. This chapter first identifies the most exploited vulnerabilities in the software, hardware and network layers of national electricity grids. It then explores the threats these systems are exposed to, based on their respective vulnerabilitie作者: blithe 時間: 2025-3-28 23:05
Daniel J. Noonan,Kenneth Henry,Michelle L. Twaroskihe west. The approach taken in the chapter includes a new strategy for working with changing county boundaries and a statistical method employing Cox proportional hazards models for repeated events. The results reveal a process of settlement diffusion in the Great Plains and demonstrate that variati作者: dissolution 時間: 2025-3-29 06:44 作者: Memorial 時間: 2025-3-29 09:26 作者: 濕潤 時間: 2025-3-29 13:21
Francesca Zappacosta,Michael J. Huddleston,Roland S. AnnanPRO-VE abstracts, while the hybrid approach integrates Word2Vec for word embedding generation and a Random Forest classifier for emotion prediction. The findings reveal a predominance of positive emotions, such as trust, anticipation, and joy, in the PRO-VE abstracts, consistent with the objective n作者: 初次登臺 時間: 2025-3-29 18:48 作者: 外觀 時間: 2025-3-29 22:49
Hogune Im,Jeffrey A. Grass,Kirby D. Johnson,Meghan E. Boyer,Jing Wu,Emery H. BresnickPRO-VE abstracts, while the hybrid approach integrates Word2Vec for word embedding generation and a Random Forest classifier for emotion prediction. The findings reveal a predominance of positive emotions, such as trust, anticipation, and joy, in the PRO-VE abstracts, consistent with the objective n作者: 問到了燒瓶 時間: 2025-3-30 00:32
Natasha Elizabeth Zachara,Win Den Cheung,Gerald Warren Hartve articles explicitly aimed at improving resource efficiency were inserted in a classification framework. The research revealed the prominence of energy efficiency objectives, machine learning algorithms, and a limited number of explored manufacturing applications and fields. However, it uncovered 作者: Blood-Vessels 時間: 2025-3-30 07:58
Jaeho Lee,Donghang Cheng,Mark T. Bedfordies to stay informed about significant events in their elderly loved ones’ daily lives and interact with them at any time. Furthermore, PRO-@GE facilitates homecare provision companies in adopting novel business models, enabling the remote management of multiple individuals and ensuring prompt respo作者: meretricious 時間: 2025-3-30 12:06 作者: disciplined 時間: 2025-3-30 15:51 作者: 緯度 時間: 2025-3-30 17:38 作者: Obscure 時間: 2025-3-30 22:18 作者: 大炮 時間: 2025-3-31 02:16 作者: freight 時間: 2025-3-31 08:23
Chitra Subramanian,Yao Xu,Carl Hirschie Johnson,Albrecht G. von Arnimtraffic, while keeping them firmly . to the urbanity of the soundscape. As becomes clear in the chapter, traffic sounds are emblematic of urbanity, but urbanity may take on different meanings depending on the aestheticisation of the work and the . of the listener.作者: Detonate 時間: 2025-3-31 10:58 作者: BOON 時間: 2025-3-31 13:59
Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Btate of a protein pointing to those phosphopeptides that require further investigation. The phosphopeptide-containing fractions are then analyzed in the second dimension by nano-ES with precursor-ion scan for the marker ion m/z 79. As the final step, direct sequencing of the phosphopeptides is perfo作者: Genetics 時間: 2025-3-31 17:52 作者: Mhc-Molecule 時間: 2025-3-31 23:16 作者: BUOY 時間: 2025-4-1 04:04
Book 20042nd editionan be specifically tagged, and an explosion in the availability of highly specific antibodies from comm- cial sources, especially antibodies raised against signaling proteins of human origin. The number of available approaches is, fortunately for those working in signaling research, far too great to作者: PATRI 時間: 2025-4-1 09:56
Laurent Kurasa; Fostering collaborative and interoperable digital models for digital twins: Methods; Fostering collaborative and interoperable digital models for digital twi978-3-031-71741-3978-3-031-71739-0Series ISSN 1868-4238 Series E-ISSN 1868-422X 作者: 熄滅 時間: 2025-4-1 11:23 作者: opalescence 時間: 2025-4-1 16:11
1064-3745 al sources, especially antibodies raised against signaling proteins of human origin. The number of available approaches is, fortunately for those working in signaling research, far too great to978-1-61737-448-7978-1-59259-816-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
