標(biāo)題: Titlebook: Shiga Toxin-Producing E. coli; Methods and Protocol Stephanie Schüller,Martina Bielaszewska Book 2021 Springer Science+Business Media, LLC, [打印本頁] 作者: 怕跛行他有限 時間: 2025-3-21 18:00
書目名稱Shiga Toxin-Producing E. coli影響因子(影響力)
書目名稱Shiga Toxin-Producing E. coli影響因子(影響力)學(xué)科排名
書目名稱Shiga Toxin-Producing E. coli網(wǎng)絡(luò)公開度
書目名稱Shiga Toxin-Producing E. coli網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Shiga Toxin-Producing E. coli被引頻次
書目名稱Shiga Toxin-Producing E. coli被引頻次學(xué)科排名
書目名稱Shiga Toxin-Producing E. coli年度引用
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書目名稱Shiga Toxin-Producing E. coli讀者反饋
書目名稱Shiga Toxin-Producing E. coli讀者反饋學(xué)科排名
作者: CODA 時間: 2025-3-21 21:30
Shiga Toxin-Producing E. coli978-1-0716-1339-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 表臉 時間: 2025-3-22 01:30 作者: genesis 時間: 2025-3-22 05:20
Identification of Shiga Toxin-Producing , Outbreaks Using Whole Genome Sequencing,iate them from sporadic cases. Here, we describe an optimized protocol to efficiently determine the genome sequences of STEC using short read Illumina technology and provide information on helpful tools for the subsequent bioinformatic analysis.作者: Benign 時間: 2025-3-22 11:59 作者: THE 時間: 2025-3-22 14:39
https://doi.org/10.1007/978-1-0716-1339-9STEC; Foodborne pathogen; Virulence factors; Human pathogenesis; Clinical diagnosis作者: FIG 時間: 2025-3-22 17:11 作者: 憤世嫉俗者 時間: 2025-3-23 01:13
1064-3745 ation advice from the experts.This volume provides a comprehensive outline of the current methods used to detect, characterize, and investigate Shiga toxin-producing .E. coli.? (STEC) and Shiga toxins.?Written in the highly successful .Methods in Molecular Biology .series format, chapters include in作者: 痛得哭了 時間: 2025-3-23 03:37
Integrated Approach for the Diagnosis of Shiga Toxin-Producing , Infections in Humans,tions include both direct isolation of the STEC from stool samples and the identification of indirect evidences based on molecular, phenotypic, and serological applications. Here, the procedures in use at the Italian Reference Laboratory for . infections are described.作者: 凌辱 時間: 2025-3-23 05:50 作者: 干涉 時間: 2025-3-23 12:44
,Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing ,–Here we describe human stem cell-derived colonoid monolayers as a reliable and reproducible model to study Shiga toxin-producing . (STEC) infection and STEC-caused pathologies of the whole colonic epithelium comprising a mixture of colonocytes, goblet, enteroendocrine, and other rare cells present in human colonic epithelial tissue.作者: uveitis 時間: 2025-3-23 15:26 作者: 暗語 時間: 2025-3-23 22:00 作者: Ergots 時間: 2025-3-23 23:56 作者: Intentional 時間: 2025-3-24 02:21 作者: 似少年 時間: 2025-3-24 07:07 作者: 貨物 時間: 2025-3-24 12:48 作者: 設(shè)施 時間: 2025-3-24 17:03 作者: 制定法律 時間: 2025-3-24 19:15 作者: 帶來 時間: 2025-3-24 23:56
Book 2021iding known pitfalls...?..Authoritative and cutting-edge,?.Shiga Toxin-Producing E. coli: Methods and Protocols .aims to be a valuable resource for clinicians, epidemiologists, and researchers interested in STEC pathogenesis..作者: DAMP 時間: 2025-3-25 06:51 作者: 聾子 時間: 2025-3-25 08:45
Integrated Approach for the Diagnosis of Shiga Toxin-Producing , Infections in Humans,diagnosis of STEC infection is of primary importance to drive the most appropriate patient’s management procedures. The methods to diagnose STEC infections include both direct isolation of the STEC from stool samples and the identification of indirect evidences based on molecular, phenotypic, and se作者: photopsia 時間: 2025-3-25 14:08
Shiga Toxin-Producing , in Animals: Detection, Characterization, and Virulence Assessment,ic potential for humans. Domestic swine can carry and shed STEC, but only STEC strains producing the Shiga toxin (Stx) 2e variant and causing edema disease in piglets are considered pathogens of veterinary medical interest. In this chapter, we present general diagnostic workflows for sampling livest作者: 上下連貫 時間: 2025-3-25 18:44
Identification of Shiga Toxin-Producing , Outbreaks Using Whole Genome Sequencing,iate them from sporadic cases. Here, we describe an optimized protocol to efficiently determine the genome sequences of STEC using short read Illumina technology and provide information on helpful tools for the subsequent bioinformatic analysis.作者: 漂白 時間: 2025-3-25 22:20
Predicting Host Association for Shiga Toxin-Producing , Serogroups by Machine Learning,w considerable diversity in gene content between isolates. It is a reasonable assumption that gene content reflects evolution of strains in particular host environments and therefore can be used to predict the host most likely to be the source of an isolate. An extrapolation of this argument is that作者: hair-bulb 時間: 2025-3-26 00:16
Isolation and Characterization of Shiga Toxin Bacteriophages,e methods used for the detection, enumeration, and isolation of Stx bacteriophages. Stx phages are temperate phages located in the genome of STEC. Their induction from the host strain cultures is achieved by different inducing agents, mitomycin C being one of the most commonly used. Detection of inf作者: Hyperalgesia 時間: 2025-3-26 06:56 作者: 箴言 時間: 2025-3-26 09:21
Functional Analysis of Shiga Toxin-Producing , Biofilm Components in Plant Leaves,orticultural crops. This becomes a public health concern for plants that are eaten raw or minimally processed, such as leafy salad and fruits. STEC actively interact with plants as hosts, and so to determine the mechanistic basis to the interaction, it is necessary to assess STEC gene function in pl作者: 無效 時間: 2025-3-26 15:46
Virulence Factor Cargo and Host Cell Interactions of Shiga Toxin-Producing , Outer Membrane Vesiclehese pathogens. STEC O157 OMVs carry a cocktail of virulence factors including Shiga toxin 2a (Stx2a), cytolethal distending toxin V (CdtV), EHEC hemolysin, flagellin, and lipopolysaccharide. OMVs are taken up by human intestinal epithelial and microvascular endothelial cells, the major targets duri作者: FAZE 時間: 2025-3-26 17:54 作者: fastness 時間: 2025-3-26 22:19
Thin-Layer Chromatography in Structure and Recognition Studies of Shiga Toxin Glycosphingolipid Rec a fatty acid (mostly C16–C24) and a sugar moiety harboring to a great extent one to five monosaccharides. GSLs of the globo-series are well-recognized receptors of Shiga toxins (Stxs) released by Stx-producing . (STEC). Receptors for the Stx subtypes Stx1a and Stx2a are globotriaosylceramide (Gb3Ce作者: 大酒杯 時間: 2025-3-27 04:26
Identification of Nanobodies Blocking Intimate Adherence of Shiga Toxin-Producing , to Epithelial CTEC) in the intestine of the patient and also in the bovine reservoir, thereby minimizing the risk of STEC contamination in the food chain. Of particular interest are recombinant single-domain Ab fragments called nanobodies (Nbs) derived from the variable domain of camelid heavy chain-only antibodie作者: 天真 時間: 2025-3-27 06:24 作者: 詳細(xì)目錄 時間: 2025-3-27 13:04
,Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing ,–gy and pathologies as they can be propagated indefinitely and preserve the genetic signature of the donor and the gut segment specificity in culture. Here we describe human stem cell-derived colonoid monolayers as a reliable and reproducible model to study Shiga toxin-producing . (STEC) infection an作者: 中世紀(jì) 時間: 2025-3-27 17:03 作者: 極微小 時間: 2025-3-27 18:04 作者: DIKE 時間: 2025-3-27 22:31
Interaction of Bovine Lymphocytes with Products of Shiga Toxin-Producing ,d proliferation and pro-inflammatory cytokine synthesis. Here we describe how to isolate lymphocyte subsets from bovine peripheral blood as well as methods that we have used to study the effects of STEC products on lymphocyte proliferation and cytokine production. We also describe an assay that allo作者: mendacity 時間: 2025-3-28 05:38 作者: frozen-shoulder 時間: 2025-3-28 06:17
Infant Rabbit Model for Studying Shiga Toxin-Producing ,toxin-producing . (STEC). The optimal model may differ depending on what aspects of pathogen biology, disease progression, or host response are under study. Here, we provide detailed protocols for the infant rabbit model of STEC, which largely reproduces the intestinal disease seen following natural作者: 蝕刻 時間: 2025-3-28 11:45
Lysogenized with a Shiga Toxin-Producing Phage: A Murine Model for Shiga Toxin-Producing , Infectiepithelium, characterized by effacement of microvilli and the formation of actin “pedestals” beneath intimately attached bacteria. In addition, STEC are lysogenized with a phage that, upon induction, can produce potent Shiga toxins (Stx), potentially leading to both hemorrhagic colitis and hemolytic作者: 茁壯成長 時間: 2025-3-28 17:27 作者: languid 時間: 2025-3-28 19:11
Isolation and Characterization of Shiga Toxin Bacteriophages,ntify and enumerate Stx phages. Real-time quantitative PCR (qPCR) is the most accurate method for absolute quantification, although it cannot determine the infectivity of Stx phages. qPCR can also be useful for the detection of free Stx phage virions in different sample types..Stx phages induced fro作者: 植物茂盛 時間: 2025-3-29 01:24 作者: overhaul 時間: 2025-3-29 06:56
Thin-Layer Chromatography in Structure and Recognition Studies of Shiga Toxin Glycosphingolipid Recerlay detection allows for sensitive identification of Stx-binding GSLs in complex mixtures directly on the TLC plate. For this purpose, specific anti-GSL antibodies or Stxs themselves in conjunction with anti-Stx antibodies can be used. The described protocols of antibody-mediated detection of TLC-作者: bonnet 時間: 2025-3-29 10:18
Identification of Nanobodies Blocking Intimate Adherence of Shiga Toxin-Producing , to Epithelial Cening methods used to isolate inhibitory Nbs blocking intimin–Tir protein–protein interaction, actin-pedestal formation, and intimate adhesion of STEC to epithelial cells in vitro. First, we describe how VHH gene repertoires can be produced as Nbs secreted by . using the α-hemolysin (HlyA) protein s作者: 多樣 時間: 2025-3-29 14:21 作者: 雪白 時間: 2025-3-29 18:13
Lysogenized with a Shiga Toxin-Producing Phage: A Murine Model for Shiga Toxin-Producing , Infectids for the use of CR(Φ.) infection as a highly predictable murine model for infection and disease by STEC. Importantly, we detail CR(Φ.) inoculation by feeding, determination of pathogen colonization, production of phage and toxin, and assessment of intestinal and renal pathology. These methods prov作者: Seizure 時間: 2025-3-29 23:35
Stefan Bletz,Alexander Mellmann,Barbara Middendorf-Baucharty has often simulated nature by analyzing complex organic substances taken from living organisms and then producing by synthesis absolutely pure compounds, which mostly consisted of only one single active subst978-3-642-26872-4978-3-642-19382-8Series ISSN 2192-3671 Series E-ISSN 2192-368X 作者: 違反 時間: 2025-3-30 03:58 作者: ANNUL 時間: 2025-3-30 04:41
Kenneth G. Campellone,Alyssa M. Coultery has often simulated nature by analyzing complex organic substances taken from living organisms and then producing by synthesis absolutely pure compounds, which mostly consisted of only one single active subst978-3-642-26872-4978-3-642-19382-8Series ISSN 2192-3671 Series E-ISSN 2192-368X 作者: 聲明 時間: 2025-3-30 12:05 作者: drusen 時間: 2025-3-30 14:27 作者: Admonish 時間: 2025-3-30 18:58
Karol Dokladny,Julie G. In,James Kaper,Olga Kovbasnjuky has often simulated nature by analyzing complex organic substances taken from living organisms and then producing by synthesis absolutely pure compounds, which mostly consisted of only one single active subst978-3-642-26872-4978-3-642-19382-8Series ISSN 2192-3671 Series E-ISSN 2192-368X 作者: Little 時間: 2025-3-30 23:31 作者: implore 時間: 2025-3-31 03:22 作者: 船員 時間: 2025-3-31 06:43
s or plants were applied to arrowheads..Over the last 110 years the pharmaceutical industry has often simulated nature by analyzing complex organic substances taken from living organisms and then producing by synthesis absolutely pure compounds, which mostly consisted of only one single active subst作者: Ingrained 時間: 2025-3-31 09:30 作者: ARIA 時間: 2025-3-31 14:00 作者: 不持續(xù)就爆 時間: 2025-3-31 18:35
Lorena Rodríguez-Rubio,Maite Muniesas or plants were applied to arrowheads..Over the last 110 years the pharmaceutical industry has often simulated nature by analyzing complex organic substances taken from living organisms and then producing by synthesis absolutely pure compounds, which mostly consisted of only one single active subst作者: inferno 時間: 2025-3-31 23:07
Nicola J. Holden,Kathryn M. Wright,Jacqueline Marshall,Ashleigh Holmess or plants were applied to arrowheads..Over the last 110 years the pharmaceutical industry has often simulated nature by analyzing complex organic substances taken from living organisms and then producing by synthesis absolutely pure compounds, which mostly consisted of only one single active subst作者: Sigmoidoscopy 時間: 2025-4-1 04:11
Martina Bielaszewska,Lilo Greune,Andreas Bauwens,Petra Dersch,Alexander Mellmann,Christian Rüters or plants were applied to arrowheads..Over the last 110 years the pharmaceutical industry has often simulated nature by analyzing complex organic substances taken from living organisms and then producing by synthesis absolutely pure compounds, which mostly consisted of only one single active subst作者: 小溪 時間: 2025-4-1 08:53
Johanna Detzner,Gottfried Pohlentz,Johannes Müthings or plants were applied to arrowheads..Over the last 110 years the pharmaceutical industry has often simulated nature by analyzing complex organic substances taken from living organisms and then producing by synthesis absolutely pure compounds, which mostly consisted of only one single active subst
