標(biāo)題: Titlebook: Selective Autophagy; Methods and Protocol Ioannis P. Nezis Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licen [打印本頁] 作者: 重要 時(shí)間: 2025-3-21 17:46
書目名稱Selective Autophagy影響因子(影響力)
書目名稱Selective Autophagy影響因子(影響力)學(xué)科排名
書目名稱Selective Autophagy網(wǎng)絡(luò)公開度
書目名稱Selective Autophagy網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Selective Autophagy被引頻次
書目名稱Selective Autophagy被引頻次學(xué)科排名
書目名稱Selective Autophagy年度引用
書目名稱Selective Autophagy年度引用學(xué)科排名
書目名稱Selective Autophagy讀者反饋
書目名稱Selective Autophagy讀者反饋學(xué)科排名
作者: Condescending 時(shí)間: 2025-3-21 21:50
Synthetic Dimerization Approaches for In Vivo Studies in ,e describe two chemically induced dimerization systems in the model organism . Using the autophagy pathway as an example, we show how these approaches can be used to dissect molecular events in cells.作者: 允許 時(shí)間: 2025-3-22 00:56
Assays to Study Mitophagy in Plants,ial marker lines followed by image analysis, chemical inhibitor treatment, and plant phenotype studies. These methods can be used in combination to identify the putative mitophagy regulators and understand their functions in mitochondrial-related activities in plants.作者: 積極詞匯 時(shí)間: 2025-3-22 05:06
Fluorescence Microscopy and Immunoblotting for Mitophagy in Budding Yeast,pregulated under prolonged respiration or nutrient starvation, anchored on the surface of mitochondria, and activated to recruit the autophagy machinery for the formation of autophagosomes surrounding mitochondria. In this chapter, we provide protocols to assess Atg32-mediated mitophagy using fluorescence microscopy and immunoblotting.作者: 新義 時(shí)間: 2025-3-22 09:13 作者: CRANK 時(shí)間: 2025-3-22 16:25
Studying Selective Autophagy of Protein Aggregates Using Particles Induced by Multimerization (PIMs use of pH-sensitive fluorescent proteins, such as GFP or mKeima, the PIM assay allows for direct visualization of aggregate clearance in cells. Here, we describe a protocol for the use of the PIM assay to study aggrephagy in live and fixed cells.作者: Control-Group 時(shí)間: 2025-3-22 19:28
High-Throughput Screening for LC3/GABARAP Binders Utilizing the Fluorescence Polarization Assay,dition, a case study on peptide interaction with human Atg8 proteins (LC3/GABARAPs) is described. Finally, data analysis and quality control of FP assays?are discussed for the proper calculation of . values for the measured compounds.作者: 以煙熏消毒 時(shí)間: 2025-3-22 21:37 作者: 按時(shí)間順序 時(shí)間: 2025-3-23 05:04 作者: gnarled 時(shí)間: 2025-3-23 06:19
1064-3745 ation advice from the experts.This volume presents the latest techniques used by researchers to study selective autophagy in a broad range of organisms such as yeast, plants, worms, fruit flies, and mammals. The chapters in this book cover several types of selective autophagy such as mitophagy, aggr作者: hieroglyphic 時(shí)間: 2025-3-23 13:47 作者: SPECT 時(shí)間: 2025-3-23 16:24 作者: myocardium 時(shí)間: 2025-3-23 19:46
Methods for Monitoring Mitophagy Using mt-Keima,rom this bimodal excitation, a ratiometric signal may be calculated to quantify mitophagy in live cells. This chapter describes procedures for measuring mitophagy by flow cytometry and live cell confocal microscopy with mt-Keima.作者: 束縛 時(shí)間: 2025-3-23 23:18
Methods for Monitoring NCOA4-Mediated Ferritinophagy,g iron homeostasis, heme synthesis, mitochondrial respiratory function, and ferroptosis, an iron-dependent form of cell death. Targeting ferritinophagy has emerged as a potential anticancer therapeutic strategy. In this context, we provide a flowchart of the procedures and accompanying protocols for monitoring ferritinophagic flux.作者: Jargon 時(shí)間: 2025-3-24 02:34 作者: resilience 時(shí)間: 2025-3-24 09:06 作者: Androgen 時(shí)間: 2025-3-24 14:23
Methods to Monitor Nucleophagy in Yeast, for maintaining nucleus homeostasis and regulating nucleus functions. In the budding yeast ., nucleophagy occurs in two different manners: one involves autophagosome formation for the sequestration and vacuolar transport of nucleus-derived vesicles (NDVs), and the other proceeds with the invaginati作者: Palpate 時(shí)間: 2025-3-24 16:07
Synthetic Dimerization Approaches for In Vivo Studies in ,n, where, and how proteins interact, making it possible to study their functions, dynamics, and cellular consequences at a molecular level. These methods are versatile, reversible, and adaptable, allowing the dissection of complex cellular processes and the engineering of cellular functions. Here, w作者: 分開 時(shí)間: 2025-3-24 19:20 作者: Adj異類的 時(shí)間: 2025-3-25 02:55
Assessing Neuronal Mitophagy During Development in the Nematode ,tive autophagic removal of mitochondria, stands out as a prominent mechanism for efficient mitochondrial turnover, which is crucial for proper neuronal development and function. Dysfunctional mitochondria and disrupted mitophagy pathways have been linked to a diverse array of neurological disorders.作者: 酷熱 時(shí)間: 2025-3-25 07:11 作者: escalate 時(shí)間: 2025-3-25 09:40 作者: 領(lǐng)巾 時(shí)間: 2025-3-25 11:46 作者: 潛移默化 時(shí)間: 2025-3-25 17:54 作者: 過多 時(shí)間: 2025-3-25 22:08 作者: ARIA 時(shí)間: 2025-3-26 01:37 作者: FLUSH 時(shí)間: 2025-3-26 07:42
Methods for Monitoring Mitophagy Using mt-Keima,a mitochondrial matrix targeting sequence and has bimodal excitation with peaks at 440?nM in neutral environments and 586?nM in acidic environments. From this bimodal excitation, a ratiometric signal may be calculated to quantify mitophagy in live cells. This chapter describes procedures for measuri作者: 不要不誠實(shí) 時(shí)間: 2025-3-26 12:09 作者: FAR 時(shí)間: 2025-3-26 16:22 作者: 猜忌 時(shí)間: 2025-3-26 20:31
Method to Purify Phase-Separated p62 Bodies Using Fluorescence-Activated Particle Sorting,important roles in intracellular quality control and stress responses. However, little is known about their constituents. In this chapter, we describe a method for purifying p62 bodies using fluorescence-activated particle sorting. This method contributes to the identification of novel components of作者: 平庸的人或物 時(shí)間: 2025-3-27 00:07
In Silico Assessment of LIR Motif-Containing Proteins Binding to ATG8-Family Proteins,mily proteins. Here, we present a detailed methodology for the in silico evaluation of potential binding between LIR motif-containing proteins and ATG8-family proteins. We visualize AlphaFold-predicted protein complexes using PyMOL to assess potential interactions, providing an effective computation作者: 遺留之物 時(shí)間: 2025-3-27 04:18
High-Throughput Screening for LC3/GABARAP Binders Utilizing the Fluorescence Polarization Assay,ecules is important for a detailed understanding of selective autophagy mechanisms and for the design of potential Atg8 inhibitors that affect the autophagy processes in cells. The fluorescence polarization (FP) assay is a rapid, cost-effective, and robust method that provides affinity and selectivi作者: 規(guī)范就好 時(shí)間: 2025-3-27 09:14
Thermodynamic Characterization of LC3/GABARAP:Ligand Interactions by Isothermal Titration Calorimetides information on the stoichiometry, affinity, and thermodynamic driving forces of interactions. This chapter exemplifies the use of ITC to investigate interactions between human autophagy modifiers (LC3/GABARAP proteins) and their interaction partners, the LIR motif-containing sequences. The purp作者: Grandstand 時(shí)間: 2025-3-27 10:01
Determination of hATG8 Binding Selectivity of AIM (Autophagy-Interacting Motif) Peptides Using Natio their biological roles, and in the case of disease-associated AIM mutations, potential pathophysiological mechanisms. Given the sometimes small differences in affinity for an individual AIM amongst the six hATG8 proteins, establishing AIM preferences can be experimentally challenging. We describe 作者: 方便 時(shí)間: 2025-3-27 16:02 作者: condemn 時(shí)間: 2025-3-27 20:11 作者: 減去 時(shí)間: 2025-3-27 23:40 作者: Original 時(shí)間: 2025-3-28 02:17
Methods in Molecular Biologyhttp://image.papertrans.cn/s/image/864309.jpg作者: incredulity 時(shí)間: 2025-3-28 06:49
https://doi.org/10.1007/978-1-0716-4067-8reticulophagy; ribophagy; Drosophila; zebrafish; pexophagy; non-mammalian model; C; elegans作者: 獨(dú)裁政府 時(shí)間: 2025-3-28 14:13 作者: 透明 時(shí)間: 2025-3-28 17:11 作者: optic-nerve 時(shí)間: 2025-3-28 19:10
In Silico Assessment of LIR Motif-Containing Proteins Binding to ATG8-Family Proteins,mily proteins. Here, we present a detailed methodology for the in silico evaluation of potential binding between LIR motif-containing proteins and ATG8-family proteins. We visualize AlphaFold-predicted protein complexes using PyMOL to assess potential interactions, providing an effective computational tool for this purpose.作者: 擁護(hù) 時(shí)間: 2025-3-29 00:59
s most often located at the origin of the internal carotid artery. Such carotid artery disease increases a person’s risk for cerebrovascular disease and stroke. It is responsible for 15% of all ischemic stroke. With changes in lifestyle and an aging population, it is likely that the prevalence of ca作者: DECRY 時(shí)間: 2025-3-29 04:44 作者: 逢迎春日 時(shí)間: 2025-3-29 11:02 作者: 滲透 時(shí)間: 2025-3-29 15:11 作者: 疏遠(yuǎn)天際 時(shí)間: 2025-3-29 19:25 作者: gangrene 時(shí)間: 2025-3-29 23:18 作者: 大笑 時(shí)間: 2025-3-30 00:31
Giel Korsten,Lukas C. Kapiteinreation of functionally similar code to overcome platform di.Start your video game development journey by learning how to build a 2D game engine from scratch. Using Java (with NetBeans as your IDE and using Java’s graphics framework) or by following along in C# (with Visual Studio as your IDE and us作者: Geyser 時(shí)間: 2025-3-30 07:50
Marisa Di Monaco,Marian Peteri,Jin Rui Lianga’s graphics framework) or by following along in C# (with Visual Studio as your IDE and using the MonoGame framework), you’ll cover the design and implementation of a 2D game engine in detail. Each class will be reviewed with demonstration code. You’ll gain experience using the engine by building a 作者: FACET 時(shí)間: 2025-3-30 09:35 作者: 辮子帶來幫助 時(shí)間: 2025-3-30 15:35
Francesco G. Barone,Sylvie Urbé,Michael J. Claguelves from systems that treat the described media payload as an opaque data file, individual systems must employ automated content processing. While specific processing methods have been optimized to handle particular media types, there are common principles that apply to some degree across all media作者: genuine 時(shí)間: 2025-3-30 19:35 作者: 正常 時(shí)間: 2025-3-30 21:26 作者: 關(guān)節(jié)炎 時(shí)間: 2025-3-31 01:50 作者: sebaceous-gland 時(shí)間: 2025-3-31 08:56 作者: expire 時(shí)間: 2025-3-31 11:43
Mamas Louca,Bridget Penman,Alexander Cameron,Ioannis P. Nezis with an infectious disease was still highly infectious after passage through a filter capable of retaining bacterial cells. From such humble beginnings the study of these ‘filter-passing agents‘, or viruses, has developed into a separate science which rivals, if it does not excel, in importance the作者: contradict 時(shí)間: 2025-3-31 15:41 作者: 滔滔不絕地講 時(shí)間: 2025-3-31 20:03 作者: 吞噬 時(shí)間: 2025-4-1 01:40
Andrew Brennan,Neil Oldham,Robert Layfield,Daniel Scottor beginners.Includes a wealth of experiments and open sourc.This book offers a systematic and comprehensive introduction to the visual simultaneous localization and mapping (vSLAM) technology, which is a fundamental and essential component for many applications in robotics, wearable devices, and au作者: monopoly 時(shí)間: 2025-4-1 02:05
Christina Karantanou,Sofia-Iris Bibliin video and image processing systems, and to conversion between the RGB color representation and the HIS, HSV and YUV/YC.b.C.r. color representations.978-1-4471-2502-0978-1-4471-2503-7Series ISSN 1863-7310 Series E-ISSN 2197-1781 作者: 懶洋洋 時(shí)間: 2025-4-1 06:37
Thuy T. P. Nguyen,Joseph D. Manciasin video and image processing systems, and to conversion between the RGB color representation and the HIS, HSV and YUV/YC.b.C.r. color representations.978-1-4471-2502-0978-1-4471-2503-7Series ISSN 1863-7310 Series E-ISSN 2197-1781
