標(biāo)題: Titlebook: SMC Complexes; Methods and Protocol Anjana Badrinarayanan Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Chro [打印本頁] 作者: NO610 時間: 2025-3-21 17:50
書目名稱SMC Complexes影響因子(影響力)
書目名稱SMC Complexes影響因子(影響力)學(xué)科排名
書目名稱SMC Complexes網(wǎng)絡(luò)公開度
書目名稱SMC Complexes網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱SMC Complexes被引頻次
書目名稱SMC Complexes被引頻次學(xué)科排名
書目名稱SMC Complexes年度引用
書目名稱SMC Complexes年度引用學(xué)科排名
書目名稱SMC Complexes讀者反饋
書目名稱SMC Complexes讀者反饋學(xué)科排名
作者: 低三下四之人 時間: 2025-3-21 21:40
1064-3745 expertsThis book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic .S.tructural .M.aintenance of .C.hromosome (SMC) proteins. The protocols explore numerous subjects such as depletion systems to assess SMC function, 作者: Exclude 時間: 2025-3-22 02:50
Knocking in Multifunctional Gene Tags into SMC Complex Subunits Using Gene Editingified and localized (multifunctional tags). In this chapter, we detail how to engineer vertebrate cells using CRISPR/Cas9 to provide researchers powerful tools to obtain greater precision than ever to understand how the complex interacts and behaves in cells.作者: 新鮮 時間: 2025-3-22 06:52
Chromosome Conformation Capture with Deep Sequencing to Study the Roles of the Structural Maintenanceir mechanism and impact on genome organization in a cellular context. Here, I describe a Hi-C protocol, a variant of C techniques, to construct genome-wide DNA contact maps for bacteria. This protocol is optimized for the gram-negative bacterium ., but it can be readily adapted for any bacterial species of interest.作者: Blood-Vessels 時間: 2025-3-22 11:22 作者: lethal 時間: 2025-3-22 16:34 作者: Pillory 時間: 2025-3-22 20:51 作者: 正常 時間: 2025-3-23 01:16
Multicomponent Yeast Two-Hybrid System: Applications to Study Protein–Protein Interactions in SMC Cots modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6.作者: 蛛絲 時間: 2025-3-23 04:26
Using Cell Cycle-Restricted Alleles to Study the Chromatin Dynamics and Functions of the Structural functions, chromatin binding pattern and functional interactions. Here, we describe the generation of the cell cycle-restricted mutants in diploid and haploid cells and the validation of their functionality with several approaches.作者: 象形文字 時間: 2025-3-23 08:34 作者: 存在主義 時間: 2025-3-23 11:41
In Vivo and In Vitro Assay for Monitoring the Topological Loading of Bacterial Condensins on DNAnsins to DNA. In addition, isolation of bacterial condensin and DNA complexes prevents any undesired interaction between them due to cross-linking reagents. These methodologies provide reproducible and reliable results for the loading of topologically bound proteins such as bacterial condensins.作者: BRUNT 時間: 2025-3-23 17:30
Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin ImmunoprecipitationhIP). Combined with the recent technological advancement in next generation sequencing, the ChIP assay can map the exact binding sites of a protein of interest across the entire genome. Here we describe a-step-by step protocol for ChIP followed by library preparation for ChIP-seq from yeast cells.作者: sparse 時間: 2025-3-23 19:00
Dissecting DNA Compaction by the Bacterial Condensin MukBnsins are well studied, the mechanism of the overall process executed by condensins, DNA compaction, remains unclear. Here, we describe some of the methods used routinely in our laboratory to understand the mechanism of DNA compaction by . condensin MukB.作者: 神圣將軍 時間: 2025-3-24 01:21 作者: 在駕駛 時間: 2025-3-24 02:45 作者: 冰雹 時間: 2025-3-24 08:34 作者: 刪除 時間: 2025-3-24 14:14 作者: Commission 時間: 2025-3-24 18:36 作者: 哄騙 時間: 2025-3-24 19:39
Identifying Functional Domains in Subunits of Structural Maintenance of Chromosomes (SMC) Complexes s called cohesin, condensin, and SMC5/6, which share the same basic architecture. The core of SMC complexes contains a heterodimer of SMC proteins, a kleisin subunit, and a set of regulatory proteins that contain HEAT and Armadillo (ARM) repeat protein–protein interaction motifs. A major challenge i作者: 不能約 時間: 2025-3-25 00:10
Multicomponent Yeast Two-Hybrid System: Applications to Study Protein–Protein Interactions in SMC Co (SMC) complexes. SMC complexes are composed of SMC proteins, kleisin, and kleisin-interacting subunits. These subunits interact in specific ways to constitute and regulate the closed structure of the complexes. Specifically, kleisin molecules bridge the SMC dimers and the kleisin-interacting subuni作者: 粘 時間: 2025-3-25 06:21 作者: 中古 時間: 2025-3-25 10:29 作者: 詞匯表 時間: 2025-3-25 14:54 作者: 陪審團(tuán) 時間: 2025-3-25 16:45 作者: Flu表流動 時間: 2025-3-25 20:26
Preparation of Cell Cycle-Synchronized , Cells for Hi-Cealed previously unsuspected levels of chromosome structure, with different types of chromosome structure facilitating function at different stages of the cell cycle. Elucidating how SMC complexes regulate these distinct types of organization is currently a key question in molecular biology..The ran作者: Paleontology 時間: 2025-3-26 02:00 作者: COLIC 時間: 2025-3-26 07:36
In Vivo and In Vitro Assay for Monitoring the Topological Loading of Bacterial Condensins on DNAdate the molecular mechanisms underlying these roles, it is crucial to determine how and where condensins are loaded to chromosomal DNA. Here, we describe in vivo and in vitro assays for monitoring the topological loading of two bacterial condensins, Smc-ScpAB and MukBEF. A key step in these assays 作者: ELUC 時間: 2025-3-26 08:41
A Protocol for Assaying the ATPase Activity of Recombinant Cohesin Holocomplexesn genome function and structure such as topoisomerases, DNA and RNA polymerases, and DNA repair enzymes contain nucleotide binding domains (NBD) which bind and eventually cleave ATP. The released energy is harnessed in various ways by these enzymes in order to fulfill their essential functions. Howe作者: 可行 時間: 2025-3-26 14:21 作者: integrated 時間: 2025-3-26 19:16 作者: LAPSE 時間: 2025-3-27 00:39 作者: HEW 時間: 2025-3-27 02:29
https://doi.org/10.1007/978-1-4939-9520-2Chromosome organization; Structural Maintenance of Chromosomes; SMC proteins; Bacterial models; Eukaryot作者: 極小 時間: 2025-3-27 05:22
978-1-4939-9522-6Springer Science+Business Media, LLC, part of Springer Nature 2019作者: 陳腐的人 時間: 2025-3-27 11:16
SMC Complexes978-1-4939-9520-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Mast-Cell 時間: 2025-3-27 14:20
Himaja Gaddipati,Marina V. Pryzhkova,Philip W. Jordan作者: 濃縮 時間: 2025-3-27 19:25 作者: thalamus 時間: 2025-3-28 01:43 作者: 抗生素 時間: 2025-3-28 04:59 作者: construct 時間: 2025-3-28 06:39
Vasso Makrantoni,Daniel Robertson,Adele L. Marston作者: bonnet 時間: 2025-3-28 11:22 作者: 夾克怕包裹 時間: 2025-3-28 16:32
Identifying Functional Domains in Subunits of Structural Maintenance of Chromosomes (SMC) Complexes idates to transposon-based random insertion mutagenesis, followed by selection of dominant negative mutants. In this chapter we list the advantages of this approach in the context of SMC complexes. We provide a detailed protocol for performing the screen in . and use data from our recently reported 作者: 娘娘腔 時間: 2025-3-28 20:47
Analysis of Cohesin Association to Newly Replicated DNA Through Nascent Strand Binding Assay (NSBA) PCR, allowing for the parallel analysis of diverse genomic regions. While originally employed to characterize the association of cohesin to nascent sister chromatids, NSBA can be applied to study other factors dynamically associating to nascent DNA.作者: 一夫一妻制 時間: 2025-3-29 01:19
A Protocol for Assaying the ATPase Activity of Recombinant Cohesin Holocomplexestocol which we have successfully employed before for assaying the ATPase activity of recombinant budding yeast cohesin holocomplexes. We believe that with small and appropriate modifications the methods described here should be applicable to other ATPase complexes.作者: 浪蕩子 時間: 2025-3-29 06:33
1064-3745 ?.Authoritative and practical, .SMC Complexes: Methods and Protocols. will greatly aid researchers looking into the mechanism of action of SMC complexes on DNA..Chapters 10 and 19 areavailable Open Access under a CC BY 4.0 license via link.springer.com..978-1-4939-9522-6978-1-4939-9520-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 轎車 時間: 2025-3-29 09:55
,Gārgī Vācaknavī of India ?????? ???????? ,. Eighth Century BCE,e. Gārgī distinguishes herself for challenging Yāj?avalkya, being rebuked and challenging him a second time. Gārgī demonstrates her mastery over the concept at dispute (.) by being able to revise her approach to the question. Gārgī philosophically demonstrates the very idea she is investigating. Her作者: fatuity 時間: 2025-3-29 14:40 作者: 揉雜 時間: 2025-3-29 17:09
Erbliche Nierenerkrankungenmal dominante polyzystische Nierenerkrankung, (umgangssprachlich Zystennierenerkrankung) und das Alport-Syndrom. Oftmals ist die Klinik klassisch, und eine genetische Untersuchung zur Diagnosestellung ist nicht zwingend erforderlich.作者: 使成整體 時間: 2025-3-29 22:06
Water and Energyul for Canadians, electricity production and distribution have deleterious effects on the environment and aquatic ecosystems. The recent trends in electricity production, especially the use of water in energy production and the significant impacts of electricity generation on Canada’s environment are examined in this chapter.作者: Synapse 時間: 2025-3-30 01:34
Masudul Alam Choudhuryind denkbar, und erst im Schlu?bild werden jene Akzente gesetzt, die dem Drama Sinn verleihen. Vom Ende her ist das Geschaute zu deuten, denn die Differenz zwischen Individualtrag?die und gesellschaftlicher Utopie scheint nur auf den ersten Blick gering, weil beide Aspekte sich in der Handlung gegen作者: 新鮮 時間: 2025-3-30 08:02 作者: SAGE 時間: 2025-3-30 10:26
Exploring Climatic Dynamics in Madhya Pradesh, India Utilizing Long-Term Gridded Data (1951–2021): Aons. The findings reveal significant fluctuations in seasonal precipitation, with a discernible upward pattern in the premonsoon season (Z?=?0.29) with a rate of 0.02?mm annually and downward patterns in the monsoon (Z?=?-0.93), winter seasons (Z?=?-0.73) and post-monsoon (Z?=?-0.76). These results 作者: 貪婪性 時間: 2025-3-30 14:12
den diagnostischen Prozess“ der ?European Association of Psychological Assessment“, den Anforderungen des BGH an Glaubhaftigkeitsgutachten.."Der Mercedes der Gutachtenliteratur" (Prof. Jürgen Hoyer) - geschrie978-3-642-35354-3作者: GLUT 時間: 2025-3-30 18:53 作者: Indicative 時間: 2025-3-30 21:04
Book 2017uecksilber verh?lt. Zink als Element kennt man seit dem 17. Jahrhundert, Cadmium seit 1817, wogegen Quecksilber schon in der Antike bekannt war. Die erstmalige Darstellung von Atomen des Coperniciums gelang 1996..作者: ARCH 時間: 2025-3-31 03:00
Hans Mork Janus m?glichst systematisch angehen. Dazu stehen ihnen zahlreiche Instrumente wie Checklisten und digitale Standortfinder zur Verfügung. Der Wert der spezifischen Standortfaktoren für die jeweiligen Unternehmen ist zuweilen einem dynamischen Wandel unterworfen. Eine umfassende Analyse und Dokumentation
