標題: Titlebook: Ribozymes and siRNA protocols; Mouldy Sioud Book 2004Latest edition Humana Press 2004 [打印本頁] 作者: 法令 時間: 2025-3-21 19:01
書目名稱Ribozymes and siRNA protocols影響因子(影響力)
書目名稱Ribozymes and siRNA protocols影響因子(影響力)學(xué)科排名
書目名稱Ribozymes and siRNA protocols網(wǎng)絡(luò)公開度
書目名稱Ribozymes and siRNA protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Ribozymes and siRNA protocols被引頻次
書目名稱Ribozymes and siRNA protocols被引頻次學(xué)科排名
書目名稱Ribozymes and siRNA protocols年度引用
書目名稱Ribozymes and siRNA protocols年度引用學(xué)科排名
書目名稱Ribozymes and siRNA protocols讀者反饋
書目名稱Ribozymes and siRNA protocols讀者反饋學(xué)科排名
作者: Bernstein-test 時間: 2025-3-22 00:19
Mouldy SioudIncludes supplementary material: 作者: Schlemms-Canal 時間: 2025-3-22 03:18
Ribozymes and siRNA protocols978-1-59259-746-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: DEI 時間: 2025-3-22 05:39 作者: 兩棲動物 時間: 2025-3-22 11:36 作者: SENT 時間: 2025-3-22 14:10 作者: moratorium 時間: 2025-3-22 19:30
Adam Roth,Ronald R. Breaker Absicherung der erreichten Marktposition notwendig. Denn vorhandene Kunden gehen kontinuierlich verloren, indem diese zu Wettbewerbern wechseln, mit anderen Unternehmen fusionieren oder sich vom Markt zurückziehen (Dalrymple 1988, S. 98). Zum anderen lassen sich dadurch Marketingziele, wie Wachstum作者: 直言不諱 時間: 2025-3-23 00:41 作者: Dissonance 時間: 2025-3-23 01:56
Emma T. Bowden,Anna T. Riegelbeziehungen hat in den letzten Jahren eine stark zunehmende Bedeutung erlangt. Hierfür ist eine Reihe von Faktoren verantwortlich. Zu ihnen geh?rt vor allem die S?ttigung vieler M?rkte mit der Notwendigkeit, anstelle der Konzentration auf Neukundengewinnung das Ertragspotenzial bestehender Kunden au作者: Glaci冰 時間: 2025-3-23 08:36
Masayuki Sano,Kazunari Tairansbesondere die jüngere Marketing-Literatur ist von zahlreichen Beitr?gen gepr?gt, die sich mit Konstrukten und Ph?nomenen von Kundenbeziehungen, wie Kundenn?he, -orientierung, -zufriedenheit und -bindung besch?ftigen. Mit dem vorliegenden Beitrag wird eine Systematisierung dieser kundenbezogenen Th作者: RAG 時間: 2025-3-23 10:53
Roger Abounader,Robert Montgomery,Harry Dietz,John Laterrabeziehungen hat in den letzten Jahren eine stark zunehmende Bedeutung erlangt. Hierfür ist eine Reihe von Faktoren verantwortlich. Zu ihnen geh?rt vor allem die S?ttigung vieler M?rkte mit der Notwendigkeit, anstelle der Konzentration auf Neukundengewinnung das Ertragspotenzial bestehender Kunden au作者: 停止償付 時間: 2025-3-23 17:13
Jason J. Fritz,Marina Gorbatyuk,Alfred S. Lewin,William W. Hauswirthnsbesondere die jüngere Marketing-Literatur ist von zahlreichen Beitr?gen gepr?gt, die sich mit Konstrukten und Ph?nomenen von Kundenbeziehungen, wie Kundenn?he, -orientierung, -zufriedenheit und -bindung besch?ftigen. Mit dem vorliegenden Beitrag wird eine Systematisierung dieser kundenbezogenen Th作者: 擺動 時間: 2025-3-23 20:13
Hiroaki Kawasaki,Masaki Warashina,Tomoko Kuwabara,Kazunari Tairabeziehungen hat in den letzten Jahren eine stark zunehmende Bedeutung erlangt. Hierfür ist eine Reihe von Faktoren verantwortlich. Zu ihnen geh?rt vor allem die S?ttigung vieler M?rkte mit der Notwendigkeit, anstelle der Konzentration auf Neukundengewinnung das Ertragspotenzial bestehender Kunden au作者: 壓碎 時間: 2025-3-24 01:12 作者: Outmoded 時間: 2025-3-24 02:29 作者: Congruous 時間: 2025-3-24 10:06
Murray J. Cairns,Lun-Quan Sunsbesondere die jüngere Marketing-Literatur ist von zahlreichen Beitr?gen gepr?gt, die sich mit Konstrukten und Ph?nomenen von Kundenbeziehungen, wie Kundenn?he, -Orientierung, -Zufriedenheit und -bindung besch?ftigen. Mit dem vorliegenden Beitrag wird eine Systematisierung dieser kundenbezogenen The作者: declamation 時間: 2025-3-24 13:53
Combination of Chemical and Enzymatic RNA Synthesis,ilding blocks in combination with standard nucleotide 5′ triphosphates (NTPs). Short oligonucleotides that terminate in guanosine effectively compete with guanosine 5′ triphosphate (GTP) as starter building blocks, and they are incorporated at the 5′-end of transcripts. Applications include producti作者: 蒼白 時間: 2025-3-24 16:13 作者: Acetabulum 時間: 2025-3-24 19:52
Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection, aliqout for each time-point) are analyzed by gel electrophoresis followed by detection (X-ray film + densitometer or phosphoimager). This is a cumbersome approach, and involves frequent preparation of fresh labeled substrate, and rather limited time resolution. Real-time monitoring with non-decayin作者: bacteria 時間: 2025-3-25 00:45
Quantification of Ribozyme Target RNA Using Real-Time PCR,re, we describe the use of real-time polymerase chain reaction (PCR) for quantification of ribozyme target transcripts. In contrast to classical quantitative PCR, real-time PCR does not require extensive manipulation or generation of relatively complex reagents, thus reducing the risk of contaminati作者: gimmick 時間: 2025-3-25 04:20 作者: 腫塊 時間: 2025-3-25 10:44
Analysis of Global Conformational Transitions in Ribozymes,zymes contain one or more helical junctions, that act as key architectural elements in the structures. A combination of comparative gel electrophoresis and fluorescence resonance energy transfer (FRET) can be used to analyze the global conformation in solution, and to study the ion-induced folding p作者: cushion 時間: 2025-3-25 15:40 作者: 極小 時間: 2025-3-25 19:03 作者: inflate 時間: 2025-3-25 22:57
Selection In Vitro of Allosteric Ribozymes,these distinct functional classes may be combined to generate multipartite ribozymes in which the catalytic and ligand-binding elements act coordinately. In this chapter, we describe a procedure known as allosteric selection, in which an RNA catalyst in conjunction with a random sequence domain is s作者: 臥虎藏龍 時間: 2025-3-26 01:06 作者: ULCER 時間: 2025-3-26 05:15 作者: Virtues 時間: 2025-3-26 09:15
Ribozyme Expression Systems,h level of production of ribozymes in vivo. The engineered tRNA.-driven ribozymes, based on computer-predicted secondary structure, were relatively stable and were transported to the cytoplasm, where they could be colocalized with their target RNA. The activity of the exported ribozymes was signific作者: FUSE 時間: 2025-3-26 15:24 作者: vanquish 時間: 2025-3-26 18:21 作者: Irrigate 時間: 2025-3-26 22:00 作者: 疲憊的老馬 時間: 2025-3-27 02:15 作者: CLEAR 時間: 2025-3-27 05:33
Maxizyme Technology,ene-therapeutic agents. However, because of the limitation for cleavable sequences within the target mRNA, in some cases conventional ribozymes have failed to exhibit precise cleavage specificity. A maxizyme is the dimer of minimized ribozymes (minizymes), which can specifically cleave two distinct 作者: 燈泡 時間: 2025-3-27 10:39
,Target-Site Selection for the 10–23 DNAzyme, abundant in mRNA, many of these potentially cleavable junctions are protected from DNAzyme activity by secondary structure. To optimise the process of target-site selection in long RNA substrates, a multiplex assay was developed for simultaneous comparative analysis of 50 or more different DNAzymes作者: Cardiac 時間: 2025-3-27 16:14
Regulation of Ribozyme Cleavage Activity by Oligonucleotides,ing. Since both regulations are sequence-specific, ribozymes containing different sequences can be positively and independently controlled by a specific oligonucleotide. Furthermore, these allosteric hairpin loops have the ability to control the activities of other functional RNAs.作者: 鋼筆記下懲罰 時間: 2025-3-27 21:47
Tetracycline-Regulated Expression of Hammerhead Ribozymes In Vivo, .-regulatory elements and antibiotics that can modulate the binding of the transactivators at low and nontoxic doses. Here, we summarize protocols to generate cell lines expressing tetracycline-regulated ribozyme constructs.作者: 食草 時間: 2025-3-28 01:17 作者: separate 時間: 2025-3-28 04:50 作者: 社團 時間: 2025-3-28 08:31
Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes,mple cleavage and ligation reactions mediated by minimal hammerhead and hairpin ribozymes under standard experimental conditions. Information is also provided to help researchers recognize and interpret more complex reaction kinetics that can be observed for ribozyme-sequence variants under a variety of reaction conditions.作者: 江湖騙子 時間: 2025-3-28 14:27
Analysis of Global Conformational Transitions in Ribozymes,s and fluorescence resonance energy transfer (FRET) can be used to analyze the global conformation in solution, and to study the ion-induced folding processes. The FRET approach can also be extended into the time domain to analyze conformational equilibria, and increasingly to the single-molecule level.作者: instate 時間: 2025-3-28 18:30 作者: oblique 時間: 2025-3-28 18:58
Identification of Efficient Cleavage Sites in Long-Target RNAs,. After two rounds, the active preselected library pool is incubated at 37°C with target RNA in the presence of magnesium, and cleavage products are directly identified on sequencing gels. The protocol identifies highly active hRz, which typically have ..s of 20–80 n., and ../.. values of 10..作者: Forehead-Lift 時間: 2025-3-29 02:53
Ribozyme Expression Systems,antly higher than that of ribozymes that remained in the nucleus. This chapter examines the methods for construction of the appropriate vector that can produce tRNA-driven ribozymes with high-level intracellular activity and for analysis of the constructs in cells.作者: 成份 時間: 2025-3-29 03:08 作者: 啤酒 時間: 2025-3-29 07:35 作者: instulate 時間: 2025-3-29 13:38 作者: ADAGE 時間: 2025-3-29 19:25 作者: BAIL 時間: 2025-3-29 22:40 作者: resilience 時間: 2025-3-30 01:55
Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection,ase or a second dye). Applications include detailed kinetic studies and optimizing reactions with a wide range of conditions and highly specific nucleic acid sequence detections, challenging other hybridization-based methods. Special applications are the introduction of catalytic nucleic acids for r作者: prostate-gland 時間: 2025-3-30 07:00 作者: BOLT 時間: 2025-3-30 09:34 作者: 笨拙的我 時間: 2025-3-30 15:32 作者: 金哥占卜者 時間: 2025-3-30 17:22 作者: 善變 時間: 2025-3-30 21:43
Book 2004Latest editionchniques cover the analysis of ribozyme structures and conformational transitions using nucleotide analog interference mapping and fluorescence resonance energy transfer, the use of ribozymes in clinical and gene therapy, and the use of ribozymes and DNAzymes in rodent models of human disease. Each 作者: 忘川河 時間: 2025-3-31 00:53
Soumitra Basu,Catherine Pazsint,Gopeswar Chowdhury作者: 夜晚 時間: 2025-3-31 05:34
Hsiu-Hua Chen,Daniela Castanotto,Jeanne LeBon,John J. Rossi,Arthur D. Riggs作者: 有危險 時間: 2025-3-31 12:20 作者: MOTIF 時間: 2025-3-31 13:56 作者: AGONY 時間: 2025-3-31 19:53
Adam Roth,Ronald R. Breakerrnehmen der Akquisition neuer Kunden umfangreiche Ressourcen widmen. Wie eine jüngere US-amerikanische Studie für verschiedene M?rkte zeigt, nutzen Verk?ufer im Durchschnitt 14% ihrer Zeit allein dafür, potenzielle K?ufer zu identifizieren (Fenemore Group 1998, S. 96).
