標(biāo)題: Titlebook: Rhinoviruses; Methods and Protocol David A. Jans,Reena Ghildyal Book 2015 Springer Science+Business Media New York 2015 high-end imaging te [打印本頁(yè)] 作者: 挑染 時(shí)間: 2025-3-21 20:00
書(shū)目名稱Rhinoviruses影響因子(影響力)
作者: fibroblast 時(shí)間: 2025-3-21 22:07 作者: Spinous-Process 時(shí)間: 2025-3-22 02:01 作者: Carminative 時(shí)間: 2025-3-22 06:35 作者: 寄生蟲(chóng) 時(shí)間: 2025-3-22 10:32
A Protocol to Express and Isolate HRV16 3C Protease for Use in Protease Assays, transformation of bacterial cells with a commercially available cDNA plasmid which can be adapted to use for 3C proteases from any other HRV serotypes. The extracted, active 3C protease can then be used for in vitro protease assays.作者: 神刊 時(shí)間: 2025-3-22 14:13
Reverse Genetic Engineering of the Human Rhinovirus Serotype 16 Genome to Introduce an Antibody-Detite-directed mutagenesis PCR to introduce the sequence for the HA antigen onto either the C or N termini of 2A protease while keeping the relevant internal cleavage sites intact. The new viral product is then cloned into a wild-type HRV16 plasmid and transfected into Ohio Hela cells to produce recombinant virus.作者: 主講人 時(shí)間: 2025-3-22 18:04
Mouse Models of Rhinovirus Infection and Airways Disease,sponses, for representative viruses from two of these groups: (1) infection of unmodified Balb/c mice with minor group rhinovirus serotype 1B (RV-1B) and (2) infection of transgenic Balb/c mice with major group rhinovirus serotype 16 (RV-16).作者: 驚惶 時(shí)間: 2025-3-22 23:28
Book 2015search in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chroni作者: GENRE 時(shí)間: 2025-3-23 03:49 作者: 圖畫(huà)文字 時(shí)間: 2025-3-23 06:23 作者: 蟄伏 時(shí)間: 2025-3-23 10:03
Application of FCS in Studies of Rhinovirus Receptor Binding and Uncoating, conditions, the dynamic processes that take place during the early steps of virus infection. We cover two important issues of rhinovirus research, the kinetics of directional RNA release, and virus-receptor interactions exemplified by using human rhinovirus type A2 (HRV-A2) as a model.作者: 排出 時(shí)間: 2025-3-23 17:43
Reverse Genetics System for Studying Human Rhinovirus Infections,ng the artificial cDNA copy of a full-length HRV genome, identifying the infectious cDNA clone isolates, and selecting the most vigorous cDNA clone isolate to serve as the standard parental clone for future molecular genetic study of the virus.作者: 縮減了 時(shí)間: 2025-3-23 19:23 作者: 有其法作用 時(shí)間: 2025-3-23 23:51 作者: defenses 時(shí)間: 2025-3-24 02:56
Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples, respiratory pathogens in most, if not all, clinical microbiology laboratories. Molecular assays are significantly more sensitive than traditional culture-based and serological methods. This advantage has led to the recognition that HRV infections are common causes for not only upper airway symptoms作者: NAV 時(shí)間: 2025-3-24 07:20
Molecular Genotyping of Human Rhinovirus by Using PCR and Sanger Sequencing,at HRV is also the major viral cause of asthma exacerbations. Genotypic assignment and identification of HRV types are of significant value in the investigation of type-associated differences in disease outcomes, transmission, and epidemiology. Here, we describe a genotyping process involving two se作者: artless 時(shí)間: 2025-3-24 11:06
Growth of Human Rhinovirus in H1-HeLa Cell Suspension Culture and Purification of Virions,ficient quantities of virus for experiments that require highly concentrated and purified virus. This chapter describes the protocols for producing a large amount of HeLa cells in suspension culture, using these cells to grow a large quantity of virus of HeLa-adapted HRV-A and -B serotypes, and maki作者: 遺傳學(xué) 時(shí)間: 2025-3-24 16:58
Propagation of Rhinovirus-C Strains in Human Airway Epithelial Cells Differentiated at Air-Liquid Ill culture. The complete genome sequences of several RV-C strains are now available but there is little information about their biological characteristics. HRV-C were first grown in organ culture, and more recently, we developed a system for culturing RV-C strains in differentiated epithelial cells 作者: Ventricle 時(shí)間: 2025-3-24 20:44 作者: 憤怒歷史 時(shí)間: 2025-3-24 23:48 作者: AMITY 時(shí)間: 2025-3-25 05:40 作者: Ingrained 時(shí)間: 2025-3-25 09:11
Proteases of Human Rhinovirus: Role in Infection,impact. Although large-scale population vaccination has proved successful in limiting or even eradicating many viruses, the more than 100 distinct serotypes mean that conventional vaccination is not a feasible strategy to combat HRV. An alternative strategy is to target conserved viral proteins such作者: largesse 時(shí)間: 2025-3-25 14:18
A Protocol to Express and Isolate HRV16 3C Protease for Use in Protease Assays,ific differences in their action. It is therefore necessary when performing in vitro protease assays to ensure that the recombinant proteases are specific to the serotype of the HRV under study. We describe a simple method for isolating HRV16 3C protease from a bacterial expression system, including作者: 嘮叨 時(shí)間: 2025-3-25 16:04 作者: 決定性 時(shí)間: 2025-3-25 22:23
Reverse Genetic Engineering of the Human Rhinovirus Serotype 16 Genome to Introduce an Antibody-Det detection difficult. Reverse genetic engineering of virus genomes to alter the wild-type genome is a powerful technique to introduce a detectable tag onto a viral protein. Here we outline a method to incorporate an influenza hemagglutinin epitope tag onto the 2A protease of HRV16. The method uses s作者: crescendo 時(shí)間: 2025-3-26 00:26 作者: LEVER 時(shí)間: 2025-3-26 05:32
Growth of Human Rhinovirus in H1-HeLa Cell Suspension Culture and Purification of Virions,large amount of HeLa cells in suspension culture, using these cells to grow a large quantity of virus of HeLa-adapted HRV-A and -B serotypes, and making highly concentrated virus stock and highly purified virions. These purified HRV virions are free of cellular components and suitable for experiments that are sensitive to cellular contaminations.作者: crescendo 時(shí)間: 2025-3-26 12:16 作者: employor 時(shí)間: 2025-3-26 14:44 作者: Hallmark 時(shí)間: 2025-3-26 17:55 作者: 個(gè)阿姨勾引你 時(shí)間: 2025-3-26 21:27 作者: farewell 時(shí)間: 2025-3-27 04:42 作者: 進(jìn)取心 時(shí)間: 2025-3-27 08:06
Nathan W. Bartlett,Aran Singanayagam,Sebastian L. Johnston作者: 西瓜 時(shí)間: 2025-3-27 11:49 作者: NOCT 時(shí)間: 2025-3-27 14:02 作者: sparse 時(shí)間: 2025-3-27 19:54 作者: observatory 時(shí)間: 2025-3-27 23:21 作者: 的是兄弟 時(shí)間: 2025-3-28 06:01
Lora M. Jensen,Erin J. Walker,Reena Ghildyalerung des Mietrechts in Kraft..Im gewerblichen Wohnungs- und Gewerbebau finden wir im Wesentlichen drei typische Unternehmermodelle: den Bautr?ger, den Generalunternehmer und den Generalübernehmer. Dabei sind die Grenzen keineswegs starr, Mischformen sind daher denkbar und oft Ergebnis der Verhandlu作者: 講個(gè)故事逗他 時(shí)間: 2025-3-28 09:05
Wai-Ming Lee,Wensheng Wang,Yury A. Bochkov,Iris Leeche Signalfunktion .(.), der mit Hilfe der Abtastimpulse als Modulationstr?gerfunktion .(.) (Bild ..1c) periodisch Proben entnommen werden. Diese Proben bilden die abgetastete (pulsmodulierte) Signalfunktion .(.) als zeitdiskrete Pulsfolge (Bild ..1d). Die Probenentnahme kann durch Multiplikation de作者: galley 時(shí)間: 2025-3-28 13:16 作者: 熄滅 時(shí)間: 2025-3-28 16:01 作者: 噴出 時(shí)間: 2025-3-28 19:22 作者: 清澈 時(shí)間: 2025-3-28 23:37
Book 2015ble protocols, and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Rhinoviruses: Methods and Protocols .seeks to serve both professionals and novices with the latest technical advances in HRV research to ultimately enable the development of therapeutic app作者: 整體 時(shí)間: 2025-3-29 06:02
Shamaila Ashraf,Rebecca Brockman-Schneider,James E. Gerneigender Tendenz zu K?uferm?rkten wurde der Absatzbereich zunehmend Engpa?bereich betrieblicher Planung. Die Antwort bestand in der Erarbeitung neuer, situationsgerechter Konzepte. Dieser Umorientierung, die zuerst in den USA einsetzte, wurde mit Einführung des Marketing entsprochen. Damit wurde sig作者: 糾纏,纏繞 時(shí)間: 2025-3-29 08:59
Wai-Ming Lee,Yin Chen,Wensheng Wang,Anne Mossereigender Tendenz zu K?uferm?rkten wurde der Absatzbereich zunehmend Engpa?bereich betrieblicher Planung. Die Antwort bestand in der Erarbeitung neuer, situationsgerechter Konzepte. Dieser Umorientierung, die zuerst in den USA einsetzte, wurde mit Einführung des Marketing entsprochen. Damit wurde sig作者: Heart-Rate 時(shí)間: 2025-3-29 15:13 作者: Lamina 時(shí)間: 2025-3-29 18:35 作者: SEVER 時(shí)間: 2025-3-29 19:51 作者: 摘要記錄 時(shí)間: 2025-3-30 00:10
Erin J. Walker,Lora M. Jensen,Reena Ghildyal, lie? in den vergangenen Jahren ein neues Signalverarbeitungsverfahren entstehen, das unter dem Begriff Impulstechnik innerhalb kurzer Zeit sowohl in die industrielle als auch in die kommerzielle Elektronik vorgedrungen ist. Bei der Entwicklung technischer Systeme st??t man bereits im 19. Jahrhunde作者: WITH 時(shí)間: 2025-3-30 05:28
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/830122.jpg作者: 健忘癥 時(shí)間: 2025-3-30 10:42 作者: correspondent 時(shí)間: 2025-3-30 12:41
978-1-4939-5533-6Springer Science+Business Media New York 2015作者: defeatist 時(shí)間: 2025-3-30 18:23 作者: 教唆 時(shí)間: 2025-3-31 00:08
David A. Jans,Reena GhildyalIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia作者: 公司 時(shí)間: 2025-3-31 04:04
Mixing Characteristics of a Gaseous Jet in a Supersonic Crossflow,rocess between fuel and supersonic air. This process has attracted increased attention since the early 1960s. The mixing characteristics of a gaseous jet in a supersonic crossflow are detailed in this chapter.作者: 女上癮 時(shí)間: 2025-3-31 08:47 作者: CURT 時(shí)間: 2025-3-31 12:23
ectly affecting cellular survival is achieved by continuous functional adjustments meeting challenges arising in the internal and external environments. Such adjustments depend on the action of feedback mechanisms, whose properties in the open- or closed-loop modality of operation are now well under
