標(biāo)題: Titlebook: Reporter Genes; A Practical Guide Donald S. Anson Book 2007 Humana Press 2007 Gene activity.Mammalian systems.Marker genes.Reporter gene ex [打印本頁(yè)] 作者: 不足木 時(shí)間: 2025-3-21 16:24
書(shū)目名稱(chēng)Reporter Genes影響因子(影響力)
作者: jet-lag 時(shí)間: 2025-3-21 22:16 作者: left-ventricle 時(shí)間: 2025-3-22 01:04
Three-Dimensional Analysis of Molecular Signals with Episcopic Imaging Techniques,t and are readily converted to volume data sets..The two methods differ in how tissue architecture is visualized and hence how specific molecular signals are detected. EFIC uses endogenous, broad-range, tissue autofluorescence to reveal specimen structure. Addition of dyes to the wax embedding mediu作者: 調(diào)色板 時(shí)間: 2025-3-22 07:19 作者: CT-angiography 時(shí)間: 2025-3-22 11:28
Subcellular Imaging of Cancer Cells in Live Mice,nuclei increased their length 1.6 times. Cancer cells in capillaries over 8 μm in diameter were shown to migrate at up to 48.3 μm/h. With the use of dual-color fluorescent cells and the Olympus OV100, a highly sensitive whole-mouse imaging system with both macrooptics and microoptics, it is possible作者: 開(kāi)頭 時(shí)間: 2025-3-22 13:10
Noninvasive Imaging of Molecular Events with Bioluminescent Reporter Genes in Living Subjects,ly luciferase (or beetle luciferase) and renilla luciferase (isolated from sea pansy). Although both these luciferases can produce light following interaction with the substrates, structurally and biochemically they are very different. Here we describe the methods and applications of firefly and ren作者: 男生如果明白 時(shí)間: 2025-3-22 20:27
Detection of Reporter Gene Expression in Murine Airways, lung spaces. For GFP fluorescence, it is important to inflate the lungs with fixative prior to freezing and subsequent sectioning. For processing of nasal tissues for β-galactosidase expression, we expand on a protocol used in previously reported gene transfer studies.作者: GRAZE 時(shí)間: 2025-3-23 00:31 作者: Dri727 時(shí)間: 2025-3-23 02:33 作者: Regurgitation 時(shí)間: 2025-3-23 05:54
,Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues,taining with horseradish peroxidase and use as a tool for genotyping. Using these protocols, localization of β-galactosidase can be visualized throughout development and in adult tissues. β-Galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage 作者: 小步走路 時(shí)間: 2025-3-23 11:09 作者: LATER 時(shí)間: 2025-3-23 16:35 作者: 潰爛 時(shí)間: 2025-3-23 20:53 作者: DAFT 時(shí)間: 2025-3-24 00:26 作者: progestogen 時(shí)間: 2025-3-24 04:57 作者: 用樹(shù)皮 時(shí)間: 2025-3-24 08:53
Autofluorescent Proteins for Flow Cytometry,ability to analyze heterologous fluorescent protein expression conveniently and noninvasively by individually interrogating cells has facilitated increasingly more sophisticated experimental designs to address important biological questions. Improved multilaser flow cytometers have allowed the fluor作者: 死亡 時(shí)間: 2025-3-24 12:53 作者: flourish 時(shí)間: 2025-3-24 14:56 作者: 捕鯨魚(yú)叉 時(shí)間: 2025-3-24 21:20 作者: FACET 時(shí)間: 2025-3-25 00:09
Green Fluorescent Protein as a Tracer in Chimeric Tissues,the origin of cells in transplantation experiments. When not linked as fusion proteins to other polypeptides, they distribute rapidly in the cytoplasm of a given cell, thus allowing real-time observations on living material. For histological analysis, previous bath fixation of whole organs or tissue作者: Explosive 時(shí)間: 2025-3-25 05:11 作者: ATP861 時(shí)間: 2025-3-25 08:12 作者: Extemporize 時(shí)間: 2025-3-25 15:28 作者: 猜忌 時(shí)間: 2025-3-25 18:16
Green Fluorescent Protein as a Tracer in Chimeric Tissues,whole sample as a consequence of form aldehyde (FA) fixation. We have therefore developed an alternative method to fix GFP in frozen tissue sections by FA vapor. This method prevents leakage and redistribution of GFP and allows any cytochemical method to be applied to unfixed adjacent serial sections.作者: perjury 時(shí)間: 2025-3-25 20:36
weil es nicht damit getan war, die Zugtiere durch eine W?rmekraftmaschine zu ersetzen. Das Rad diente beim Pferdefuhrwerk nicht zum Antrieb. Das war beim Antrieb durch eine W?rmekraftmaschine anders, die Antriebskraft mu?te von den R?dern übertragen werden. Beim Pferdefuhrwerk wurde die Zugkraft vo作者: Conscientious 時(shí)間: 2025-3-26 01:56 作者: Figate 時(shí)間: 2025-3-26 04:51 作者: keloid 時(shí)間: 2025-3-26 09:00 作者: Daily-Value 時(shí)間: 2025-3-26 14:42
,Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues,taining with horseradish peroxidase and use as a tool for genotyping. Using these protocols, localization of β-galactosidase can be visualized throughout development and in adult tissues. β-Galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage studies.作者: –LOUS 時(shí)間: 2025-3-26 18:59
Detection of GFP During Nervous System Development in ,,development on a cellular and molecular level. In the following chapter we describe how to prepare . embryos or larvae for live imaging or immunohistochemical staining and provide some guidelines for optimal GFP detection.作者: 字形刻痕 時(shí)間: 2025-3-26 22:55 作者: COMMA 時(shí)間: 2025-3-27 01:12 作者: 嚴(yán)厲譴責(zé) 時(shí)間: 2025-3-27 06:15
Julian M. Alston,Abigail M. Okrentimportant aspect of VR-based systems is the stimulation of the human senses – usually sight, sound, and touch – in such a way that a user feels a sense of presence in the virtual environment. Sometimes it is important to combine real and virtual objects in the same real or virtual environment. This 作者: harpsichord 時(shí)間: 2025-3-27 09:29
Wolfgang Grundmann,Klaus Schüttelhat they could be given a faster ‘priority’ regulatory review within the agency. Priority review could be awarded to any new drug submitted to the agency for marketing approval, irrespective of the seriousness of the condition it was intended to treat, provided that it was deemed to promise signific作者: 殺子女者 時(shí)間: 2025-3-27 15:09
Increasing Engagement in a Cyber-Awareness Training Game. A cyber-aware mindset must be instilled in improved training across the workforce. We are developing a training game designed to improve cyber awareness with the goal of inculcating “cyber mindset” to reduce vulnerabilities to and increase vigilance toward cyber threats. The game stresses critical作者: Range-Of-Motion 時(shí)間: 2025-3-27 21:08 作者: 過(guò)分自信 時(shí)間: 2025-3-27 23:22
An Unexpected Journey: From Experimentalist to the Human Developmental Biology Resource,nvolved in its inception in 1999 and ultimately became the Resource’s co-Director for nearly 15?years. How did my scientific journey lead me to this position? I started my career as a research scientist in 1980, following the traditional pattern of Ph.D. then post-doctoral positions, initially study作者: heterodox 時(shí)間: 2025-3-28 03:53
Role of Prolactin in Stress-Induced Biological Modifications in Animals,sychological nature. Swingle et al. (1951) first described the occurrence of pseudopregnancy in female rats subjected to different types of stressor stimuli. Later, it was found that stress promotes milk secretion, suggesting the possible involvement of PRL (Nicoll et al., 1960). In 1965, Grosvenor 作者: 謙虛的人 時(shí)間: 2025-3-28 07:07 作者: 行乞 時(shí)間: 2025-3-28 11:43
Robust Image Watermarking on Triangle Grid of Feature Pointstector. Much attention is paid to the problem of choosing the most appropriate feature points detector. Conducted experiments demonstrate robustness of the proposed method to a range of geometric distortions.作者: 衰弱的心 時(shí)間: 2025-3-28 17:57
Molecular Structure of , Progenitor Toxinsbecause it is highly toxic only when administered parenterally; ingestion of derivative toxin would not cause botulism. “Botulism” is the term used to designate a neuroparalytic disease such as that caused by oral ingestion of sausages containing the toxin.作者: 群島 時(shí)間: 2025-3-28 22:41 作者: Palpate 時(shí)間: 2025-3-28 23:19 作者: ANNUL 時(shí)間: 2025-3-29 06:32
Chiam Joon Tong Ph.D., M.I.E.S. P. Eng. (Singapore)Aufbewahrungsfristen aus gesetzlichen Bestimmungen. Das Selbstbestimmungsrecht des Patienten verlangt, diesem Einsicht in die ihn betreffenen Krankenunterlagen zu gew?hren. Ausnahmen sind denkbar, wenn die Gefahr der Selbstsch?digung durch den Patienten droht.作者: 情感 時(shí)間: 2025-3-29 07:40
Early Warning of Water-Triggered Landslides,g of landslides triggered by extreme weather on regional scale, which has proven to be effective. The criteria for issuing an early warning are based on the degree of saturation of soil and the supply of water to it through rainfall and snow melting. On other hand, monitoring of single slopes on loc作者: EXCEL 時(shí)間: 2025-3-29 14:41 作者: ERUPT 時(shí)間: 2025-3-29 16:49 作者: 和藹 時(shí)間: 2025-3-29 23:43
erschienen sind. Der Verlag stellt mit diesem Archiv Quellen für die historische wie auch die disziplingeschichtliche Forschung zur Verfügung, die jeweils im historischen Kontext betrachtet werden müssen. Dieser Titel erschien in der Zeit vor 1945 und wird daher in seiner zeittypischen politisch-ide作者: 積極詞匯 時(shí)間: 2025-3-30 02:31
