標(biāo)題: Titlebook: Recombinant Proteins from Plants; Methods and Protocol Jacqueline MacDonald,Igor Kolotilin,Rima Menassa Book 2016Latest edition Springer Sc [打印本頁] 作者: commotion 時間: 2025-3-21 16:41
書目名稱Recombinant Proteins from Plants影響因子(影響力)
書目名稱Recombinant Proteins from Plants影響因子(影響力)學(xué)科排名
書目名稱Recombinant Proteins from Plants網(wǎng)絡(luò)公開度
書目名稱Recombinant Proteins from Plants網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Recombinant Proteins from Plants被引頻次
書目名稱Recombinant Proteins from Plants被引頻次學(xué)科排名
書目名稱Recombinant Proteins from Plants年度引用
書目名稱Recombinant Proteins from Plants年度引用學(xué)科排名
書目名稱Recombinant Proteins from Plants讀者反饋
書目名稱Recombinant Proteins from Plants讀者反饋學(xué)科排名
作者: Brain-Imaging 時間: 2025-3-21 20:57 作者: 慢慢流出 時間: 2025-3-22 02:57
Production of Recombinant Cholera Toxin B Subunit in , Using GENEWARE, Tobacco Mosaic Virus Vectored proteins. CTBp is purified to >95 % homogeneity by two-step chromatography using immobilized metal affinity and ceramic hydroxyapatite resins. This procedure yields on average 400 mg of low-endotoxin CTBp from 1 kg of fresh leaf material.作者: 缺陷 時間: 2025-3-22 07:52
Chloroplast-Based Expression of Recombinant Proteins by Gateway? Cloning Technology the past decade. Several improvements of the technology have turned plant plastids into robust and dependable expression platforms for multiple high value compounds. In this chapter, we describe our current methodology based on Gateway. recombinant cloning, which we have adapted for plastid transfo作者: collateral 時間: 2025-3-22 12:38
Multigene Engineering in Rice Using High-Capacity , BIBAC Vectorsnts via .-mediated transformation. Here, we describe an optimized protocol for transformation of japonica rice (. L.) using this system. Calli derived from mature embryos are transformed using . strain LBA4404 that carries the BIBAC vector and the super-virulent helper plasmid pCH32. Transformed cal作者: 大喘氣 時間: 2025-3-22 15:59
Virus-Derived Vectors for the Expression of Multiple Proteins in Plants The pEAQ vectors are a series of small binary vectors designed for controlled expression of multiple proteins in plants. To achieve high levels of expression, an expression system based on translational enhancement by the untranslated regions of RNA-2 from cowpea mosaic virus (CPMV), named CPMV-., 作者: frivolous 時間: 2025-3-22 19:40
Transient Protein Expression by Agroinfiltration in Lettuceld and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to havi作者: Ligament 時間: 2025-3-22 21:50
Production of Recombinant Proteins in the Chloroplast of the Green Alga ,rmation of . followed by protein detection. Genes of interest integrate stably by homologous recombination into the chloroplast genome following introduction by particle bombardment. Genes are inherited and expressed in lines recovered after selection in the presence of an antibiotic. Recombinant pr作者: 口味 時間: 2025-3-23 01:57
Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semi modified crops. We provide a protocol for genetic transformation of two important crops, durum wheat and alfalfa, using a bacterial and a plant-derived selectable marker gene encoding mutated, gabaculine-insensitive glutamate 1-semialdehyde aminotransferase (GSA) enzymes. These methods can potentia作者: 狗舍 時間: 2025-3-23 06:56 作者: 胡言亂語 時間: 2025-3-23 09:43 作者: 發(fā)芽 時間: 2025-3-23 16:43
Production of Recombinant Cholera Toxin B Subunit in , Using GENEWARE, Tobacco Mosaic Virus Vector Infectious transcripts of the vector RNA are generated . and inoculated on . seedlings. After 11 days, CTBp is extracted in a simple tris buffer at room temperature. No protease inhibitor is required. The leaf homogenate is treated with mild heat and a pH shift to selectively precipitate host-deriv作者: Morphine 時間: 2025-3-23 22:03
Total Soluble Protein Extraction for Improved Proteomic Analysis of Transgenic Rice Plant Rootsextractions using multistep protocols have been shown to be effective to achieve better proteome profiles than simple, single-step extractions. These protocols are generally efficient for above ground tissues such as leaves. However, each step leads to loss of some amount of proteins. Additionally, 作者: Talkative 時間: 2025-3-23 23:53
Temporary Immersion Bioreactors for the Contained Production of Recombinant Proteins in Transplastomeld cultivation. There is a need for a cost-effective, scalable process to grow large quantities of transplastomic plant biomass for biosynthesis of biopharmaceuticals and other high-value heterologous proteins. Temporary immersion culture is a means of achieving this under fully contained condition作者: lethal 時間: 2025-3-24 02:26
Plant Cell-Based Recombinant Antibody Manufacturing with a 200 L Orbitally Shaken Disposable Bioreacthe scaled-up cultivation of human IgG–secreting BY-2 cells in a 200 L orbitally shaken disposable bioreactor, resulting in cell growth and recombinant protein yields that are proportionately comparable with those obtained from cultivations in 500 mL shake flasks. Furthermore, we present an efficien作者: 巨大沒有 時間: 2025-3-24 07:59
Liquid-Liquid Phase Separation of Oil Bodies from Seedshospholipid membrane and an outer shell of specialized proteins known as oleosins. Oil bodies possess a number of attributes that were exploited by SemBioSys Genetics to isolate highly enriched fractions of oil bodies through liquid-liquid phase separation for a number of commercial applications. Th作者: 偽證 時間: 2025-3-24 10:58
Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture Extract separation (ATPS) is an attractive system to capture hydrophobin fusion proteins from plant extracts. The process can simultaneously purify and concentrate target protein with minimal background. ATPS avoids the use of chromatographic column steps, can be carried out in a short time frame, and is a作者: V切開 時間: 2025-3-24 15:17 作者: 范圍廣 時間: 2025-3-24 22:24 作者: Trochlea 時間: 2025-3-25 03:01
Multigene Engineering in Rice Using High-Capacity , BIBAC Vectors from mature embryos are transformed using . strain LBA4404 that carries the BIBAC vector and the super-virulent helper plasmid pCH32. Transformed calli are then regenerated using optimized media and tested for transgene integration by PCR, GUS assay, and Southern blot analyses.作者: 討人喜歡 時間: 2025-3-25 05:54
Virus-Derived Vectors for the Expression of Multiple Proteins in Plantspression, an expression system based on translational enhancement by the untranslated regions of RNA-2 from cowpea mosaic virus (CPMV), named CPMV-., is used. The expression vector pEAQ-. combines the user-friendly pEAQ plasmid with CPMV-. to provide a system for high-level expression of proteins in plants.作者: 緊張過度 時間: 2025-3-25 08:13 作者: Demonstrate 時間: 2025-3-25 14:25 作者: 意外 時間: 2025-3-25 16:05 作者: neuron 時間: 2025-3-25 22:03 作者: 愛社交 時間: 2025-3-26 04:13
Transient Protein Expression by Agroinfiltration in Lettuceor pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.作者: HEW 時間: 2025-3-26 08:01
Total Soluble Protein Extraction for Improved Proteomic Analysis of Transgenic Rice Plant Roots that include root tissue. For protein/proteome analysis of transgenic plant roots or of seedlings, which include root tissue, such pronounced protein degradation is especially undesirable. A facile protein extraction protocol is presented, which ensures that despite the inclusion of root tissues there is minimal loss in total protein components.作者: Ischemic-Stroke 時間: 2025-3-26 09:45
Temporary Immersion Bioreactors for the Contained Production of Recombinant Proteins in Transplastom propagation of plastid or nuclear transformants, though is especially suitable for transplastomic biomass, as organogenesis leads to greater expression and accumulation of transplastomic proteins due to increases in chloroplast number and size.作者: prosperity 時間: 2025-3-26 14:38 作者: Mechanics 時間: 2025-3-26 18:17
Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture Extractwever, this can be avoided by incorporating continuous systems, which are often preferred by the processing industry. This method chapter illustrates the capture of GFP-HFBI hydrophobin fusion protein from BY-2 plant cell suspension extract using a semi-continuous ATPS method.作者: Cursory 時間: 2025-3-26 21:15 作者: liaison 時間: 2025-3-27 01:24
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/824105.jpg作者: Arthropathy 時間: 2025-3-27 06:27
https://doi.org/10.1007/978-1-4939-3289-4cloning; molecular farming; plant expression systems; plants; recombinant proteins; transgenic plants作者: 過于平凡 時間: 2025-3-27 10:15 作者: Obstacle 時間: 2025-3-27 14:15 作者: 使饑餓 時間: 2025-3-27 20:47 作者: 撫慰 時間: 2025-3-27 23:10
Johanna Gottschamel,Andreas L?sslndung der erwachsenenp?dagogischen Diskussion der Professionstheorie unter praxisbezogener Sicht ansatzweise aufzuzeigen. Ein solcher Versuch ist angesichts der gegenw?rtigen erziehungswissenschaftlichen Diskussion der Professionstheorie, die obschon sie ausnahmslos sozialwissenschaftlichen Frageste作者: 跑過 時間: 2025-3-28 05:14 作者: Contracture 時間: 2025-3-28 09:14 作者: 不怕任性 時間: 2025-3-28 13:34
Qiang Chen,Matthew Dent,Jonathan Hurtado,Jake Stahnke,Alyssa McNulty,Kahlin Leuzinger,Huafang Laind eine gemeinsame Bedeutungs-Wurzel mit ‘Gefahr’.. Sp?testens seit den Diskussionen um die Gefahren und Risiken der wissenschaftlich-technischen Entwicklung, den Resultaten des Experimentierens, steht der faktische Zusammenhang auch im Mittelpunkt einer Auseinandersetzung um die Bedeutung und Rolle作者: Prostatism 時間: 2025-3-28 17:48 作者: 菊花 時間: 2025-3-28 21:04
in den Sinn kommen, wenn man an Strafvollzugsanstalten denkt, sind wahrscheinlich diejenigen der Einschlie?ung und der Absonderung. Doch was ‘dort’ auch noch geschieht und insbesondere, welche “Mikrophysik der Macht” (Foucault 1976) im Anschlag ist, erf?hrt man natürlich nur, wenn man sich dem Trei作者: 小丑 時間: 2025-3-29 02:01 作者: figurine 時間: 2025-3-29 04:09
Manish L. Raorane,Joan O. Narciso,Ajay Kohliach concerned with some aspect of the relationship between human expertise, statistical modeling, and knowledge-based systems, in particular expert systems. The first section provides a comparison of human expert judgment with statistical models, particularly linear regression models, concluding tha作者: 沒有貧窮 時間: 2025-3-29 07:43
Sherwin Barretto,Franck Michoux,Peter J. Nixonmodels and the expert judgment of the managers who would use them. This objective had been motivated by the apparent lack of success at the everyday managerial level that twenty-five years or so of operations research and management science model building had achieved. The OR and MS tradition had be作者: Mirage 時間: 2025-3-29 11:36
Nicole Raven,Stefan Schillberg,Stefan Raschemodels and the expert judgment of the managers who would use them. This objective had been motivated by the apparent lack of success at the everyday managerial level that twenty-five years or so of operations research and management science model building had achieved. The OR and MS tradition had be作者: 壁畫 時間: 2025-3-29 19:06 作者: CHAR 時間: 2025-3-29 22:39
Liquid-Liquid Phase Separation of Oil Bodies from Seeds to this technology was oil body isolation consisting of simple manipulation including homogenization of seeds to free the oil bodies, followed by the removal of insoluble fractions, and phase separation to recover the oil bodies. During oil body enrichment (an increase in oil body content concomita作者: MAL 時間: 2025-3-30 01:55 作者: 擴音器 時間: 2025-3-30 04:45
Kurniawan Rudi Trijatmiko,Felichi Mae Arines,Norman Oliva,Inez Hortense Slamet-Loedin,Ajay Kohli作者: condone 時間: 2025-3-30 08:58 作者: enhance 時間: 2025-3-30 14:13 作者: conduct 時間: 2025-3-30 17:06
Johanna Gottschamel,Andreas L?sslnspruchnahme wissenssoziologischer Theoriebildung reflektiert. Sodann wird auf wissenssoziologische Bezugspunkte der Inhaltsdidaktik eingegangen. Anschlie?end werden die sich daraus ergebenden Folgerungen für die Fortbildungsdidaktik anhand einer empirischen Analyse verdeutlicht, wobei auch auf meth作者: cornucopia 時間: 2025-3-30 22:04 作者: Alcove 時間: 2025-3-31 03:23 作者: 竊喜 時間: 2025-3-31 07:04
Qiang Chen,Matthew Dent,Jonathan Hurtado,Jake Stahnke,Alyssa McNulty,Kahlin Leuzinger,Huafang Laion Gutachten und Begutachtungen Begründungsarbeit für andere leisten, und da? Gutachter als unabh?ngige gedacht und behandelt werden k?nnen. Die Inhalte ihrer Begründungen sind jedoch abh?ngig von einem komplexen disziplin?ren Beziehungsgeflecht, in dem ihr Wissen in der Wahl bestimmter Annahmen und
